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Articles by Francisco de Assis Machado Reis
Total Records ( 1 ) for Francisco de Assis Machado Reis
  Arildo Jose Braz de Oliveira , Luzia Koike , Francisco de Assis Machado Reis , Silvia Yuko Eguchi , Eliana Harue Endo , Celso Vataru Nakamura and Benedito Prado Dias Filho
  Screening tests of hydroethanolic crude extracts of six species of Aspidosperma (Apocynaceae) against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa were performed. Aspidosperma ramiflorum Muell. Arg. showed good activity against Bacillus subtilis with MIC and MBC of 15.7 and 125μg/mL, moderate activity against Staphylococcus aureus with MIC and MBC of 250 and 500μg/mL, and weak activity against Escherichia coli with MIC and MBC of 1000μg/mL. Aspidosperma pyricolum Muell. Arg. (MIC/MBC 125/250μg/mL) and Aspidosperma olivaceum Muell. Arg. (MIC/MBC 250/>1000μg/mL) displayed moderate antibacterial activity against Bacillus subtilis. Separation of the crude extract of Aspidosperma ramiflorum was performed according to the usual acid-base process, which produces alkaloid mixtures and closely related metabolites. The basic fraction was active against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli, with MICs of 31.2, 62.5, and 250μg/mL, respectively. The basic fractions were more active than the acid fractions, probably because they contained some active alkaloids and/or closely related metabolites absent from the other fractions, or they contained a higher concentration of these active compounds.
 
 
 
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