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| Articles
by
Florin GHIURU |
Total Records (
3 ) for
Florin GHIURU |
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Ileana MICLEA
,
Marius ZAHAN
,
Vasile MICLEA
,
Andrea HETTIG
,
Iulian ROMAN
and
Florin GHIURU
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As defence against oxidative stress, living systems employ antioxidants that they either produce or take up from the environment. Among these α-tocopherol has been shown to improve swine oocyte maturation and the development of bovine and swine embryos. The goal of this study was to establish the influence of several α-tocopherol concentrations on swine embryo development in vitro, in order to improve culture media. Pig oocytes were cultured for 45 hours at 37°C in 5% CO2 atmosphere; in M199 containing several α-tocopherol (5, 10, 20, 40 and 80 μM) concentrations. Then, they were fertilized in TALP medium using spermatozoa capacitated by centrifugation and incubated for 16-18 hours. Afterwards, the presumed zygotes were cultured in NCSU-23 droplets supplemented with α-tocopherol (5, 10, 20, 40 and 80 μM) in the same conditions. Their development was assessed at 48 and 120 hours. The number of embryos that had developed to the 2 cells, 4-8 cells and morula stages was compared to the control, and the differences analyzed using the analysis of variance and interpreted using the LSD and Duncan tests. At 48 hours after fertilization it was apparent that α-tocopherol supplementation increased embryo development to the 4-8 cell (10, 20, 40, 80 μM) and morula (5, 40, 80 μM) stages. At 120 hours even more embryos had reached the morula stage. |
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Ileana MICLEA
,
Marius ZAHAN
,
Vasile MICLEA
,
Andrea HETTIG
,
Iulian ROMAN
,
Florin GHIURU
and
Elena ILISIU
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As defence against oxidative stress, living systems employ antioxidants that they either produce or take up from the environment. Among these lutein has been shown to be a powerful antioxidant that also functions as a signal molecule. The goal of this study was to establish the influence of several lutein concentrations on swine embryo development in vitro, in order to improve culture media. Pig oocytes were cultured for 45 hours at 37°C in 5% CO2 atmosphere; in M199 containing several lutein (2.5, 4, 5, 8, and 10 μM) concentrations. Then, they were fertilized in TALP medium using spermatozoa capacitated by centrifugation and incubated for 16-18 hours. Afterwards, the presumed zygotes were cultured in NCSU-23 droplets supplemented with lutein (2.5, 4, 5, 8, and 10 μM) in the same conditions. Their development was assessed at 48 and 120 hours. The number of embryos that had developed to the 2 cells, 4-8 cells and morula stages was compared to the control, and the differences analyzed using the analysis of variance and interpreted using the LSD and Duncan tests. Lutein (2.5 μM) supplementation has a beneficial effect on embryo development to the morula stage. This is apparent at 48 hours as well as 120 hours. |
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Marius ZAHAN
,
Vasile MICLEA
,
Florin GHIURU
,
Iulian ROMAN
,
Alexandru RUSU
,
Ileana MICLEA
and
Manuel MIHAILESCU
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Despite the new researches in boar semen cryopreservation, application of frozenthawed
(FT) semen in commercial artificial insemination (AI) programs is still limited. The
main sources of semen used in AI are semen preserved on room temperature (RT) and
sometimes fresh (F) semen. However, the use of male germplasm from genebank on some
rare breeds is connected with the ability to use frozen semen. The aim of this study was to
evaluate the effect of semen preservation method and especially the influence of freezing on
Red Mangalitsa and Bazna boar semen cryopreservation.
Six sexually mature boars from Red Mangalitsa and Bazna rare breeds of the Faculty
of Animal Sciences and Biotechnology, USAMV Cluj-Napoca, were used in this study.
Semen was collected using the gloved-hand method, evaluated for conventional semen
characteristics and extended in Beltsville Thawing Solution (BTS). Preservation was made by
RT and by frozen using a modified method described by Westendorf et al. (1975), in lactose,
egg yolk, glycerol and Orvus ES Paste extender (LEYGO). The F, RT and FT sperm was
evaluated by Eosin-nigrosin viability staining, acrosomal integrity shown by normal apical
ridge (NAR) and plasma membrane integrity by hypo-osmotic swelling test (HOST) in order
to establish sperm quality. Because it is known that the life of sperm is short in utero after
thawing, the FT sperm quality was also determined by thermoresistance test (motility at 10,
30, 60, 120 and 240 minutes). The results were statistically analyzed using ANOVA. The first
parameter affected by freezing was viability, but the state of the acrosome has shown
significant differences between the preservation methods on both breeds. In comparison with
F and RT semen the value of NAR was halved. In addition, membrane integrity spermatozoa
(HOST) was significantly lower after the FT procedure compared to F and RT.
Thermoresistance test has shown a good motility until 120 minutes, with the best value at 30
minutes, but decreased dramatically at 240 minutes. However, this results correlated with the
reducing insemination-to-ovulation interval could improve the fertility after AI with Red
Mangalitsa and Bazna breeds’ semen cryopreservation |
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