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Articles by Feng Yan
Total Records ( 4 ) for Feng Yan
  Jianhong Hu , Feng Yan , Zhuoying Zhang and Jinyang Lin
  This study was designed to evaluate the antioxidant and anti-fatigue activities of Ganoderma lucidum Polysaccharides (Gl-PS). In vitro antioxidant activities of Gl-PS were investigated through DPPH and superoxide radical scavenging activities. In vivo anti-fatigue activity of Gl-PS was investigated through forced swimming test of mice. Results showed that Gl-PS had strong scavenging activity to DPPH and superoxide radical. In vivo experimental studies showed that Gl-PS had anti-fatigue activity which could evidently extend exhaustive swimming time of mice and decrease the blood lactate and serum urea nitrogen contents. The results provided an important basis for developing the Gl-PS as a novel antioxidant and anti-fatigue compound.
  Hongqiao Li , Abuzar Ghafoor , Hassan Karim , Shixing Guo , Zhuang Li , Yongcheng Wu , Yuanyuan Sun and Feng Yan
  Background and Objective: For cultivation and high yield of oilseed rape (Brassica napus L.) in China, traditional seedling transplanting is replaced by seed-sowing but, better nitrogen management is crucial and not established yet. This study aimed to adapt N management to the seed-sowing method for the winter oilseed rape and to minimize the N fertilizer-derived pollution potential in the upper reaches of Yangtze River Basin. Materials and Methods: Three field experiments were conducted to check effect of different doses of N fertilizers, split doses of N and different types of N fertilizers for seed-sowing winter oilseed rape with high plant density in upper reaches of Yangtze River Basin in Sichuan province of China. Results: In first experiment, among four doses (0, 90, 180 and 270 kg N ha1) on average 3.54 t ha1 was in 180 kg N ha1 and 3.61 t ha1 in 270 kg N ha1 while cultivars dy6 and cn3 produced 3.23 and 3.29 t ha1 which is significantly higher than zs11. There was no significant difference in N-use efficiency among three cultivars tested and second experiment showed no significant difference in seed yield with split N application. The third experiment compared the effects of different fertilizer types (urea, coated urea, 1:1 mixture of urea and coated urea and compound nitrogen fertilizer) on seed yield and get no significant difference in seed yield. Conclusion: This experiment proved that seed sowing method with higher nitrogen had high yield in the upper reaches of Yangtze River Basin in China, but higher N application may cause environment pollution. So, seed sowing method with nitrogen 180 kg N ha1 was proved to be more effective.
  Mei Zhu , Fengsong Wang , Feng Yan , Phil Y. Yao , Jian Du , Xinjiao Gao , Xiwei Wang , Quan Wu , Tarsha Ward , Jingjing Li , Steve Kioko , Renming Hu , Wei Xie , Xia Ding and Xuebiao Yao
  Chromosome segregation in mitosis is orchestrated by dynamic interaction between spindle microtubules and the kinetochore. Septin (SEPT) belongs to a conserved family of polymerizing GTPases localized to the metaphase spindle during mitosis. Previous study showed that SEPT2 depletion results in chromosome mis-segregation correlated with a loss of centromere-associated protein E (CENP-E) from the kinetochores of congressing chromosomes (1). However, it has remained elusive as to whether CENP-E physically interacts with SEPT and how this interaction orchestrates chromosome segregation in mitosis. Here we show that SEPT7 is required for a stable kinetochore localization of CENP-E in HeLa and MDCK cells. SEPT7 stabilizes the kinetochore association of CENP-E by directly interacting with its C-terminal domain. The region of SEPT7 binding to CENP-E was mapped to its C-terminal domain by glutathione S-transferase pull-down and yeast two-hybrid assays. Immunofluorescence study shows that SEPT7 filaments distribute along the mitotic spindle and terminate at the kinetochore marked by CENP-E. Remarkably, suppression of synthesis of SEPT7 by small interfering RNA abrogated the localization of CENP-E to the kinetochore and caused aberrant chromosome segregation. These mitotic defects and kinetochore localization of CENP-E can be successfully rescued by introducing exogenous GFP-SEPT7 into the SEPT7-depleted cells. These SEPT7-suppressed cells display reduced tension at kinetochores of bi-orientated chromosomes and activated mitotic spindle checkpoint marked by Mad2 and BubR1 labelings on these misaligned chromosomes. These findings reveal a key role for the SEPT7-CENP-E interaction in the distribution of CENP-E to the kinetochore and achieving chromosome alignment. We propose that SEPT7 forms a link between kinetochore distribution of CENP-E and the mitotic spindle checkpoint.
  Yong Yang , Fang Wu , Tarsha Ward , Feng Yan , Quan Wu , Zhaoyang Wang , Tanisha McGlothen , Wei Peng , Tianpa You , Mingkuan Sun , Taixing Cui , Renming Hu , Zhen Dou , Jingde Zhu , Wei Xie , Zihe Rao , Xia Ding and Xuebiao Yao
  Chromosome movements in mitosis are orchestrated by dynamic interactions between spindle microtubules and the kinetochore, a multiprotein complex assembled onto centromeric DNA of the chromosome. Here we show that phosphorylation of human HsMis13 by Aurora B kinase is required for functional kinetochore assembly in HeLa cells. Aurora B interacts with HsMis13 in vitro and in vivo. HsMis13 is a cognate substrate of Aurora B, and the phosphorylation sites were mapped to Ser-100 and Ser-109. Suppression of Aurora B kinase by either small interfering RNA or chemical inhibitors abrogates the localization of HsMis13 but not HsMis12 to the kinetochore. In addition, non-phosphorylatable but not wild type and phospho-mimicking HsMis13 failed to localize to the kinetochore, demonstrating the requirement of phosphorylation by Aurora B for the assembly of HsMis13 to kinetochore. In fact, localization of HsMis13 to the kinetochore is spatiotemporally regulated by Aurora B kinase, which is essential for recruiting outer kinetochore components such as Ndc80 components and CENP-E for functional kinetochore assembly. Importantly, phospho-mimicking mutant HsMis13 restores the assembly of CENP-E to the kinetochore, and tension developed across the sister kinetochores in Aurora B-inhibited cells. Thus, we reason that HsMis13 phosphorylation by Aurora B is required for organizing a stable bi-oriented microtubule kinetochore attachment that is essential for faithful chromosome segregation in mitosis.
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