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Articles by Fei Wang
Total Records ( 6 ) for Fei Wang
  Quanyu Yu , Kangkang Zhang , Yawei Pan and Fei Wang
  The study analyzes the factors that cause failure of air-conditioning system and builds the corresponding fault tree. The failure spectrum of air-conditioning system is constructed through qualitative analysis of the fault tree. And theoretical researches of the quantitative analysis results are also carried out. The study provides the theoretical foundation of automobile air-conditioning system’s design and maintenance.
  Min Lai , Hui-juan Zhang , Fei Wang , Ya-lan Shao , Mei-wen Yang , Fen-fang Hong and Shu-long Yang
  Senescence is the comprehensive manifestation of human body deterioration induced by various environmental factors. Ginsenosides are the main anti-aging ingredients of ginseng. Ginsenoside Rg1 protects the nervous system from aging by promoting human intelligence, inhibiting the release of LDH and MDA and decreasing intracellular calcium overload. Loss of memory and learning ability resulting from aging can be resisted by ginsenoside Rg1, Rb1 and Rg2. Ginsenoside are effective in inhibiting neuronal cell apoptosis and promoting proliferation of these cells. These molecules also inhibit the aging process regulated by the Rb and p53-dependent pathways and exert their function against Alzheimer's disease. In addition, experimental results from animals demonstrated that ginseng saponins protected the brain cortex and regulated TrkB mRNA expression in the hippocampus. This paper reviewed all recent developments in the study of the anti-aging effects of ginseng and its ginsenosides on neural systems to provide a theoretical basis for the clinical application of these molecules in the prophylaxis and treatment of diseases related to the neural system aging.
  Xiao-Hong Wu , Ye Liu , Lin Zhang , Fei Li , Fei Wang , Li Cao , Xiao-Jun Yang and Jun-Hu Yao
  This experiment was conducted to investigate the effects of natural Vitamin E (Nat E Ac) level and duration of supplementation on growth performance, carcass traits, meat quality and α-tocopherol content as well as oxidation stability of plasma and breast meat (refrigerated at 4°C) of broilers. Cobb broilers (n = 315, 21 days old, female) were randomly assigned to 7 treatments (1 control and 6 experiment groups) with 5 replicates and 9 broilers per replicate. Control group was fed with basal diet (Nat E Ac:30 IU kg-1). For the experimental groups, a 2x3 factorial design was used with 2 Nat E Ac levels (Nat E Ac:60 and 120 mg kg-1) and 3 durations (7, 14 and 21 days prior to slaughter at 42 day). The broilers were in the finisher phase (22-42 days) during the treatment. Results showed that broilers fed with higher Nat E Ac levels or fed for a longer duration had significantly (p<0.05) increased percentage of breast and thigh meat, pH24h in breast, glutathione peroxidase in liver and α-tocopherol in plasma, liver and breast meat. Moreover, the broilers also exhibited notably (p<0.05) decreased drip loss and pH in breast meat as well as decreasing content of Malondialdehyde (MDA) in the plasma and breast meat refrigerated at 4°C for 0, 2, 4, 6 and 8 days. There was a significant (p<0.05) interaction between Nat E Ac level and feeding duration that affected the level of MDA in breast meat refrigerated at 4°C for 0, 2, 6 and 8 days. Nat E Ac level, feeding duration and the interaction of these two factors did not markedly (p>0.05) influence growth performance, breast meat color, percentage of eviscerated carcass and abdominal fat, total superoxide dismutase and glutathione peroxidase activities and antioxidation capacity in plasma. Higher Nat E Ac level or prolonged duration of feeding increased the percentages of breast and thigh meat, enhanced the water-holding capacity, the pH24h as well as α-tocopherol retention of breast meat and oxidative stability during refrigerated storage of breast meat thus enhancing the oxidative stability during refrigerated storage and improving breast meat quality.
  Shen He , Chong Feng , Chuan Long , Hongmei Pan , Ning Wang , Ningning Shi , Fei Wang , Yanrong Zhou , Hongxing Chen , Mingxing Chu , Dengke Pan and Xuewei Li
  The development of α-1,3-galactosyltransferase gene knockout pigs along with overexpression of human Complement Regulatory Proteins (hCRPs) has been an important step in overcoming hyperacute rejection when pig organs are transplanted into nonhuman primates. Most previous studies have used commercial pigs as organ donors. However, little has been reported about the use of inbred miniature pigs as genetic donors expressing hCRPs. As expression of hCRP on the surface of pig cells, especially human membrane cofactor protein (hCD46) could effectively protect them against human complement-mediated rejection, researchers constructed an engineered hCD46 minigene expression vector and generated transgenic Wuzhishan miniature Pigs (WZSP) expressing hCD46. Expression of hCD46 with a distinct tissue-specific pattern similar to the human one was observed using immunohistochemistry. Various tissues revealed strong immunostaining in most epithelial tissues and vascular endothelium. Flow cytometry analysis of hCD46 porcine AECs showed expression levels similar to human AECs. Complement-mediated cytolysis of transgenic porcine Aortic Endothelial Cells (AECs) with human serum showed significant protection. Distinct differences between ABO blood groups were also observed with much less cytolysis of porcine AECs treated with B blood group serum than with other serum types. In conclusion, transgenic WZSPs with sufficient expression of hCD46 have been established successfully and will facilitate further xenotransplantation research.
  Fei Wang , Xiao-Yuan Wu , Rong-Min Yu , Zhen-Guo Zhao and Can-Zhong Lu
  1-(2-Pyridyl)benzotriazole (L1) and 1-(4-pyridyl)benzotriazole (L2) with transition metal cations Co(II), Ni(II) and Cu(II) give four coordination complexes, [{Cu(L1)2(H2O)2} · 2NO3] (1), [{Co(L1)2(H2O)2} · 2NO3] (2), [Ni(L2)2(NO3)2(H2O)2] (3), and [Cu2(L2)2(CH3COO)4] (4). In 1-4, different supramolecular frameworks are formed through hydrogen bonding and/or π-π interactions.
  Kristen M. Stewart-Maynard , Margareta Cruceanu , Fei Wang , My-Nuong Vo , Robert J. Gorelick , Mark C. Williams , Ioulia Rouzina and Karin Musier-Forsyth
  Human immunodeficiency virus type 1 (HIV-1) nucleocapsid protein (NC) is a nucleic acid chaperone that facilitates the remodeling of nucleic acids during various steps of the viral life cycle. Two main features of NC`s chaperone activity are its abilities to aggregate and to destabilize nucleic acids. These functions are associated with NC`s highly basic character and with its zinc finger domains, respectively. While the chaperone activity of HIV-1 NC has been extensively studied, less is known about the chaperone activities of other retroviral NCs. In this work, complementary experimental approaches were used to characterize and compare the chaperone activities of NC proteins from four different retroviruses: HIV-1, Moloney murine leukemia virus (MLV), Rous sarcoma virus (RSV), and human T-cell lymphotropic virus type 1 (HTLV-1). The different NCs exhibited significant differences in their overall chaperone activities, as demonstrated by gel shift annealing assays, decreasing in the order HIV-1 ~ RSV > MLV >> HTLV-1. In addition, whereas HIV-1, RSV, and MLV NCs are effective aggregating agents, HTLV-1 NC, which exhibits poor overall chaperone activity, is unable to aggregate nucleic acids. Measurements of equilibrium binding to single- and double-stranded oligonucleotides suggested that all four NC proteins have moderate duplex destabilization capabilities. Single-molecule DNA-stretching studies revealed striking differences in the kinetics of nucleic acid dissociation between the NC proteins, showing excellent correlation between nucleic acid dissociation kinetics and overall chaperone activity.
 
 
 
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