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Articles by Fazal Hadi
Total Records ( 2 ) for Fazal Hadi
  Gowher Ali , Fazal Hadi , Zahir Ali , Muhammad Tariq and Muhammad Ali Khan
  In the present study, regeneration conditions for two-cultivars-of-tobacco (Nicotiana tabacum L.) were optimized. At different concentrations the effects of 1-naphthalene acetic acid (auxin)-and-6-benzylaminopurine (cytokinin)-on-callus-induction-and-subsequent-plant regeneration in K-399 and SPTG-172 using Murashige and Skoog (MS) media were studied. The seeds were germinated on simple MS media, while MS media with auxine and cytokinin was used for callus induction and regeneration of shoots. In tobacco, callus was successfully induced from leaf explants using a high ratio of auxin: Cytokinin. The over all callus induction frequency in SPTG-172 was greater than K-399 indicating a difference in the genetic background of two cultivars. Both cultivars also showed best response to different hormonal concentrations. The resultant calli were stimulated to form shoots by increasing the cytokinin concentration and decreasing auxin content of culture media. There were clear differences between the two cultivars in their ability to produce regenerated plants. The maximum number of calli were regenerated in SPTG-172 whereas the mean number of shoots produced per callus at all concentrations was higher in K-399 indicating that’s shoot regeneration is markedly affected by the genotype. The roots developed well on hormones free MS media in both cultivars irrespectively of the varietal difference. The well-rooted plants were then transferred to green house for acclimatization-to-the in vitro environment.
  Fazal Akbar , Fazal Hadi , Zakir Ullah and Muhammad Amir Zia
  A green house study was conducted at the University of Malakand, NWFP, Pakistan to evaluate the effect of marble industry effluent on soil pH, germination, post germinative growth and productivity of maize. The experiment was conducted in triplicate form for each treatment and tape water was used as control (T0). Effluents were diluted with tap water at concentration of 20% (T1), 40% (T2), 60% (T3), 80% (T4) and also used 100% (T5) concentration in 4 kg soil potG1 and plants were grown for 90 days. Results showed that there was a linear increase in pH of soil with increase in effluent concentration while germination, root length and stem girth was enhanced and found maximum at 40% concentration of effluent applied. The shoot length and root dry biomass was depressed as compared to control. It is concluded from the present study that marble industry effluent can be used as a fertilizer in low concentration especially for highly acidic soil but there is still need to carry out series of greenhouse and field trials to ascertain the fertilizer potentials of this effluent for maize crop.
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