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Articles by Fatimah Md. Yusoff
Total Records ( 4 ) for Fatimah Md. Yusoff
  M. Aminur Rahman , Fatimah Md. Yusoff , A. Arshad , S.M.N. Amin and Mariana Nor Shamsudin
  An attempt was undertaken to describe the size frequency distribution, length weight relationships and fecundity estimates of short-spined sea urchin, Salmacis sphaeroides from Peninsular Malaysia. In total 355 specimens of S. sphaeroides were collected from the inter-tidal shoal of Tanjung Kupang, Johor during the breeding season in March-August 2011. For each individual, the Total Length (TL) was measured using digital slide calipers and individual Body Weight (BW) was also taken through a digital balance. Mean length of S. sphaeroides was estimated as 72.85 mm and the mean weight was 143.01 g. Among the 355 specimens measured, 158 were males and 197 were females, indicating a sex ratio of 1 male to 1 female (1:1.25). The logarithmic form of length-weight relationship of S. sphaeroides was LogW = 2.4396xLogTL-2.3958. The exponential form of equation obtained for the length-weight relation was W = 0.004xTL2.4396. The value of regression co-efficient (R2) estimated for the species was 0.77. The mean fecundity was estimated at 7,676,000 (±247773) eggs. The regression coefficient between the absolute Fecundity (F) and Total Length (TL) was 0.76 and between the fecundity and drained body weight (DW) was 0.89; revealed linear regression model with a positive and significant relationship. This study represents the first time results on the total length-body weight relationships and fecundity estimates of this sea urchin from Peninsular Malaysia. The findings would immensely be helpful towards the understanding of growth patterns and fecundity, which will ultimately facilitate to develop the breeding, larval rearing and aquaculture of sea urchins.
  M. Aminur Rahman , A. Arshad , Fatimah Md. Yusoff and S.M.N. Amin
  Potential for interspecific hybridization between genetically diverged species of tropical sea urchins, Echinometra sp. A (Ea) and Echinometra mathaei (Em) was examined through cross fertilization and hybrid rearing experiments. Mean performance traits of fertilization, larval survival, metamorphosis and recovery of juveniles Em (ova)xEa (sperm) and Ea (ova)xEm (sperm) hybrids were not significantly different from each other but were significantly lower than either of their conspecific control, EaxEa and EmxEm. Despite these, hybrids in both directions were developed normally to sexually mature adults. The growth parameters (final weight, weight gain, gonad weight, gonad index and SGR) of 2-year-old adult hybrids were significantly higher than the superior parent (EaxEa) and inferior parent (EmxEm). The gonad production showed an increment of 45.49% in F1 hybrids over mid-parents, while it showed an increase of 33.74%, 62.60% and 46.76% in F1 hybrid of EmxEa and 31.42, 59.79 and 44.22% in F1 hybrid of EaxEm over the superior, inferior and mid-parents, respectively. Survival was highest in EmxEm followed by EaxEa, EmxEa and EaxEm in that order. Therefore, body growth, gonad production and survival indicate hybrids in either direction were viable in laboratory conditions. The superiority of these growth traits of the hybrid groups over their parental values indicates positive heterosis (hybrid vigor). This study is the first successful demonstration of hybrid vigor between two diverged species of sea urchins. Hence hybrids in both directions appear to have considerable potential for use in aquaculture.
  N. Maya Erna , Sanjoy Banerjee , Mohamed Shariff and Fatimah Md. Yusoff
  The immobilization of nitrifying bacteria in alginate has been used to evaluate the performance of ammonia reduction. In this research, bacteria were screened and observed for their ability to reduce ammonia. Consortium M1, isolated from the mangrove area (Morib, Selangor) showed the most effective reduction of ammonia from an initial concentration of 2.17±0.10 to 0.06±0.01 mg L-1 in 14 days. The consortium was then identified to consist of Pseudomonas aeruginosa (99%), Pseudomonas stutzeri (98%) and Nocardioides albus (98%) using the 16S rDNA gene sequences via Polymerase Chain Reaction (PCR) technique and identified by phylogenetic analysis based on their partial 16S rDNA sequences comparison in NCBI GenBank. The consortium M1 was then immobilized into alginate beads each containing 1.79x103 CFU mL-1 bacteria cells before being tested for its efficacy in reducing ammonia under laboratory conditions using 10, 20, 30, 40, 50, 100, 150 and 200 beads. The fastest reduction rate of Total Ammonia Nitrogen (TAN) was observed in flasks containing 150 beads which on day 6, drastically reduced TAN from 2.50±0.10 to 0.090±0.059 mg L-1 followed by treatment with 200 beads (0.104±0.07 mg L-1). However, at the end of experiment at day 14, the lowest TAN level (0.083±0.063 mg L-1) was observed in flasks with 200 beads which was not significantly different (p>0.05) from flasks with 150 beads (0.106±0.034 mg L-1). The present study reveals that the immobilization of bacterial consortium consisting of ammonia oxidizing bacteria could be used as an alternative for reduction of high TAN concentration in shrimp or fish hatchery system.
  M. Srikanth Reddy , Fatimah Md. Yusoff , B. Naresh and Prathibha Devi
  Polycyclic Aromatic Hydrocarbons (PAHs) are persistent hydrophobic organic pollutants ubiquitously found in the environment. PAHs consist of cytotoxic, mutagenic and carcinogenic properties and causes serious hazard to human health and environment. Hence, in this investigation eight phenanthrene (250 mg L-1) degrading bacterial strains were isolated by enrichment method from petroleum contaminated sludge samples. Among these isolates, one efficient degrading strain was identified and characterized by using morphological, biochemical, chemotaxonomic (FAMEs analysis) and molecular (16S rDNA sequencing) methods. Based on these studies the strain was identified as gram positive, motile, spore forming, pink pigmented cocci with oxidase negative reaction and utilized sugars such as arabinose, xylose and rhamnose as carbon source. It is susceptible to kanamycin, nalidixic acid and novobiocin type of antibiotics. The dominant fatty acids found in this strain are 15:0 anteiso (44.16%), 15:0 iso (26.36%). Phylogenetic analysis of this strain showed 99% sequence similarity with Kocuria rosea and the strain name as PDM-7 was adopted. The degradation study illustrates that this strain utilizing about 82% of phenanthrene in six days. Therefore, this strain can be efficiently used for the bioremediation of PAHs contaminated marine environments and soils.
 
 
 
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