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Articles by Farooq Latif
Total Records ( 3 ) for Farooq Latif
  Nighat Aslam , Farooq Latif , Sibtain Ahmed and Amer Jamil
  Trichoderma harzianum (E-58) was grown on Vogel`s medium containing glucose and xylan as carbon source. Glucose repressed the β-glucosidase production while xylan produced the enzyme in substantial amounts. Total RNA was isolated from mycelia of Trichoderma harzianum grown in medium containing xylan as carbon source. RT-PCR was performed on the isolated RNA. cDNA strand was synthesized by using oligo (dT)18 primer. Specific primers were used to amplify bgl gene from cDNA. The bgl gene was ligated at SmaI site of pUC18 and transformed into E. coli.
  Kishwar Hayat , Haq Nawaz , Farooq Latif and M. Asghar
  The purpose of present study was to determine the effect of dissolved O2 on cellulase and xylanase production by Chetomium thermophile in growth medium of wheat straw. The concentration of dissolved oxygen was optimized through stirring. Kinetic parameters including substrate utilization (g substrate L/h) and enzyme production (IU) was studied from the maximum slope in plots of substrate utilized (g/L) and enzyme produced (IU/L) vs time of fermentation. Specific rate of product formation (QP) and substrate utilization (Qs) was also determined.
  Maliha Rashid , Samina Khalil , Najma Ayub , Sadia Alam and Farooq Latif
  After screening a large number of phosphate solubilizing microorganisms (PSM) isolated from rice rhizosphere on the basis of solubilization index (SI) and pH drop ten best bacterial strains (10RB, 54RB, 57RB, 20RB, 21RB, 23RB, 22RB, 13RB, 20RB, 40RB) and three best fungal strains {(Aspergillus flavus(1RF), Penicillium canescens(4RF), A. niger (8RF)}were further analyzed for their acid production ability using HPLC technique. SI of 13 best isolates ranged from 2.16-6.23. SI and change of pH of these isolates showed abrupt changes during seven days of incubation in Pikovskayas agar and broth medium respectively. This drop of pH clearly indicated the production of organic acids. HPLC results confirmed that few PSM isolates produced gluconic, fumaric, succinic, acetic and some unknown organic acids in smaller concentrations while oxalic and citric acids in larger concentrations in their broth medium. Among bacterial strains 10RB produced largest amount of organic acids (2.4747 g-l) while among fungal isolates Aspergillus flavus produced greatest amount of acids (1.835 g-l) than the other two fungal strain. The concentration of glucose when analyzed during HPLC was found less in all the cultural samples as compared to the control (13 g-l) that was without any PSM culture. This shows that all the PSM strains utilized the carbon of glucose for the production of organic acids. The abilities of these efficient strains for phosphorus (P) solubilization and immobilization were also quantitatively investigated in Pikovskayas broth medium. The results showed that all the strains solubilized (0.04-0.1%) and immobilized (0.1-0.4%) P but fungi immobilized more P than bacteria. Correlation among all the parameters showed a positive(r = 0.2, P<0.1) correlation between the organic acid excretion and P solubilization and a negative correlation was found between pH and P solubilization (r =-0.4, P<0.1) and immobilization (r=-0.5, P<0.05).
 
 
 
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