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Articles by Fariba Sharififar
Total Records ( 7 ) for Fariba Sharififar
  Ali Vaziri Gohar , Abbas Mohammadi and Fariba Sharififar
  P53 tumor suppressor protein is a key component of genome guard. The p53 inactivation occurs frequently in many human cancers by mutation or over-expression of its specific inhibitor named MDM2 oncoprotein (Murine Double Minute-2), thus MDM2 inhibitors might offer the preventive and therapeutic strategies. In contrast, ATM (Ataxia Telangiectasia Mutated) tumor suppressor protein activates p53 protein. The MDM2 inhibitors such as Nutlin-3, HLI98 and MDM2 antisense oligonucleotides have been identified, but clinical validation is still pending. The Zataria multiflora Boiss. has antioxidant and antibacterial effects. To determine the correlation between the Zataria multiflora Boiss. and anti-carcinogenesis effects, we investigated the role of the essential oil of this plant on MDM2 and ATM genes expression using semi-quantitative RT-PCR in liver samples of 8 normal male rats. The results clearly showed that ATM and MDM2 gene expression increased and decreased, respectively, although the difference in ATM mRNA between target and control groups did not reach levels of significance. Our data implicated the essential oil of Zataria multiflora Boiss. as a down-regulator of MDM2 gene expression and may offer new tools for therapeutic intervention in the p53 pathway for cancer prevention and treatment.
  Bijan Naghibi , Vahid Sheibani , Mostafa bagherinia , Gholamreza dehghan-nudeh and Fariba Sharififar
  Ghavoot is an Iranian preparation composed of a combination of different plant seeds which has been prepared in a specific manipulation. This drug has different traditional uses which has never been studied scientifically. In the present study, the anti anxiety effect of Ghavoot has been studied in mice using Elevated Zero Maze (EZM) method. The animals received the Ghavoot at doses of 12, 18 and 24 g kg-1 orally for 14 days. The anxiolytic effect of test groups was compared to diazepam on the basis of animal behaviors on 15th day of experiment. The time spent in the open arms, number of open arm entries, number of animal stretching, line crossing and deep heading of animal on open edges were measured for 5 min. All of the experiment were done at the time of 9-13 a.m. The results showed that Ghavoot significantly (p<0.05) elevated the time spent on the open arms and open arm entries at dose of 24 g kg-1 in comparison to control group, without significant changes in the other paramaters. The results here provide scientific evidence that Ghavoot has potential anti anxiety effect in mice.
  Ali Assadipour , Fariba Sharififar , Maryam Robati , Vida Samzadeh and Khadijeh Esmaeilpour
  Essential oils are a kind of secondary metabolites with a variety of valuable biological activities. In the present study, the essential oils of the flowering and fruiting tops of Ducrosia assadii Alava. (Apiaceae), were isolated separately by steam distillation and studied by Gas Chromatography (GC) and Gas Chromatography Mass Spectrometry (GC/MS). Antioxidant effect of the essential oils was studied using diphenylpicrylhydrazil (DPPH) scavenging assay. The results indicated some quantitative but not qualitative differences between the analyzed oils. The major components of the oil were found to be n-decanal 46.68 and 42.21%, (n-decanol ) 13.79, 13.27% and (dodecanal) 10.01 and 8.8%, respectively in flowers and fruits. In both studied oils, oxygenated aliphatic compounds comprise the highest percentage of the oil (76.88, 77.71% in flower and fruit oil, respectively) while the sesquiterpenes were at low percentage in both oils. Both these oils exhibited relative antioxidant effect in comparison to butylated hydroxyanizole (BHA).The composition of the essential oil of D. assadii was completely different from the other studied species of the plant such as D. anethifolia and D. esmaeli which could be accounted for their biologic activities.
  Gholamreza Dehghan-Noodeh , Fariba Sharififar , Mohammad Hassan Moshafi , Effat Behravan , Ali Dehghan-Noodeh and Reza Rezaei-Gharaeei
  Cancer and some other mutation-related diseases are still remained among the most difficult ones of clinical aspects. Regards to the mention issue attention is drawn to dietary anti-mutations as cancer preventative agents. The presence of anti-mutagenic agents in medicinal plants such as Zataria multiflora, Achillea wilhelmsii and Camellia sinensis can be considered due to their antioxidants properties. So, in the present work, different fractions of these plants were assessed for their anti-mutagenic effects using Ames method. Aerial parts of the tested plants after collection, drying and milling were extracted using petroleum ether, chloroform and 80% methanol consecutively by percolation method. The dried extracts under vacuum were examined for their anti-mutagenic effects. The minimum inhibition concentration (MIC) of the extracts was evaluated by ager dilution method. Various concentrations under their measured MIC were used for anti-mutagenic test. Each extract along with bacterial strain and mutagen agent were incubated at 37°C for 48 h. The number of revertant colonies was counted and compared with control plates. Our results showed that all fractions of Z. multiflora exhibited anti-mutagenicity effect in the presence of TA98, while only its methanolic fraction was active against TA100. Methanolic and chloroform fractions of C. Sinensis showed strong anti mutagenicity against TA98 only, while the methanolic fraction of A. wilhelmsii showed anti-mutagenicity effect in the presence of both TA98 and TA100. Considering the presence of flavonoids in methanolic fraction and high antimutagenicity effect of this fraction, flavonoids are probably responsible for anti-mutagenic effect of the plants.
  Mehdi Ansari-Dogaheh , Fariba Sharififar , Ali Mohammad Arabzadeh , Mojtaba Shakibaie and Mahnaz Heidarbeigi
  Rosmarinic Acid (RA) is an interesting phenolic acid with a variety of biological activities such as antioxidant, anti inflammatory and anti viral effects. In the present study, we aimed to study the inhibitory effect of methanolic extract of Zhumeria majdae against Herpes simplex type 1 (HSV-1). This extract has been standardized on the basis of RA content. Aerial parts of Zhumeria majdae was extracted with methanol 80% by maceration method. Calibration curve of RA was prepared and content of plant extract was measured on the basis of this curve by spectrophotometry method. Neutral red method was used for determining of maximum non toxic concentration (MNTC) of the plant extract. A serial dilution of plant extract up to MNTC were examined in vitro on vero cells for their anti HSV-1effect using a plaque reduction assay. Acyclovir was used as positive control. For studying of time-dependent antiviral effect of Z. majdae, plant extract was added to HSV-1 infected vero cells at different stages of infection. The percentage of RA was determined as 1.3% in Z. majdae. This plant could inhibit plaque formation at a period of 3 h after cell infection at all used concentrations (5, 10, 20 and 50 μg mL-1). This extract revealed both a time and concentration inhibition against HSV-1. Considering the antiviral effect of Z. majdae against HSV-1, can conclude that this plant is a good candidate for further studies and rosmarinic acid would be an important factor for this activity.
  Fariba Sharififar , Mansour Mirtajadini , Mohammad Jaber Azampour and Ehsan Zamani
  One of the most common strategies in the treatment of cognitive disorders is enhancing the acetylcholine level in the brain through the inhibition of acetylcholinesterase. Despite the effectiveness of current modern drugs, more attention has been paid for finding new anticholinesterase agents from medicinal plants. Zatraia multiflora Boiss. is an endemic plant to Iran which has different uses in traditional medicine as anti-inflammatory, antimicrobial, anti spasmodic. We intended to evaluate the in vitro anticholinesterase and free radical scavenging activity of the essential oil and methanolic extract of Z. multiflora. The essential oil and methanolic extract of the plant were evaluated for anticholinesterase activity using modified Ellman method. The free radical scavenging effect of the samples were studied by using of the diphenylpicrylhydrazyl (DPPH). IC50 and the percent of inhibition of acetylcholinesterase was calculated from regression equation. The results showed that both the essential oil and methanolic extract of the plant exhibited high anticholinesterase activity (95.3±3.4 and 87.9±2.2% inhibition, respectively) which was similar to eserine (96.2±1.7% inhibition). The IC50 value of essential oil was determined as 0.97±0.12 μg mL-1 in comparison to eserine (0.13±0.02 μg mL-1). The results of antioxidant assay showed that both the essential oil and methanolic extract potentially inhibit DPPH free radical (94.8±2.4 and 93.2±1.7% inhibition, respectively). The essential oil and methanolic extract of Z. multiflora have beneficial effect in health promotion and this plant would be good candidate for further studies.
  Ali Mohammad Arabzadeh , Mehdi Ansari-Dogaheh , Fariba Sharififar , Mojtaba Shakibaie and Mahnaz Heidarbeigi
  In Rosmarinic Acid (RA) is a phenolic acid which has many biological activities such as antioxidant, anti inflammatory and anti viral effects. In the present study, we have studied the anti Herpes simplex type 1 (HSV-1) effect of methanolic extract of Zataria multiflora which has been standardized on the basis of RA content. Methanolic extract of Zatria multiflora was prepared by maceration method. RA content of plant extract was measured by spectrophotometry method using the calibration curve of RA. Maximum non Toxic Concentration (MNTC) of the plant was determined by neutral red method. MNTC and lower serial dilutions of extract were examined in vitro on vero cells for their effect against HSV-1 using a plaque reduction assay. Acyclovir was used as positive control. Time-dependent antiviral effect of Z. multiflora was studied by adding the extract to HSV-1 infected vero cells at different stages of infection. The percentage of RA was determined as 2.2% in Z. multiflora. This plant was effective in all used concentrations and significantly reduced plaque formation up to 100% at concentrations of 800 and 1000 μg mL-1. Clearly Z. multiflora revealed both a time and concentration inhibition. It seems that the presence of rosmarinic acid would be a determining factor for anti HSV activity of Z. multiflora.
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