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Articles by Fang Fang
Total Records ( 7 ) for Fang Fang
  Fang Fang , Sarah Flynn , Yin Li , Marcus J. Claesson , Jan- Peter van Pijkeren , J. Kevin Collins , Douwe van Sinderen and Paul W. O’Toole
  The genome of Lactobacillus salivarius UCC118 comprises a 1.83-Mb chromosome, a 242-kb megaplasmid (pMP118), and two smaller plasmids of 20 kb (pSF118-20) and 44 kb (pSF118-44). Annotation and bioinformatic analyses suggest that both of the smaller plasmids replicate by a theta replication mechanism. Furthermore, it appears that they are transmissible, although neither possesses a complete set of conjugation genes. Plasmid pSF118-20 encodes a toxin-antitoxin system composed of pemI and pemK homologs, and this plasmid could be cured when PemI was produced in trans. The minimal replicon of pSF118-20 was determined by deletion analysis. Shuttle vector derivatives of pSF118-20 were generated that included the replication region (pLS203) and the replication region plus mobilization genes (pLS208). The plasmid pLS203 was stably maintained without selection in Lactobacillus plantarum, Lactobacillus fermentum, and the pSF118-20-cured derivative strain of L. salivarius UCC118 (strain LS201). Cloning in pLS203 of genes encoding luciferase and green fluorescent protein, and expression from a constitutive L. salivarius promoter, demonstrated the utility of this vector for the expression of heterologous genes in Lactobacillus. This study thus expands the knowledge base and vector repertoire of probiotic lactobacilli.
  Zhao Zhonghui , Zheng Xiaowei and Fang Fang
  Ganoderma lucidum, a popular medicinal mushroom, has been widely used as a herbal medicine for promoting health and longevity in China and other oriental countries. Ganoderma lucidum polysaccharides (GL-PS) are its main active components and have a lot of pharmaceutical activities. The present study was designed to determine the effects of GL-PS on exercise-induced fatigue in male Kunming mice. The mice were divided into four groups (three GL-PS administered groups and the control group). The control group were administered with distilled water and GL-PS administered groups were administered with GL-PS (50, 100 and 200 mg kg-1 b.wt.). After 28 days, anti-fatigue effects was evaluated using a forced swimming test, along with the determination of Blood Lactic Acid (BLA), Serum Urea Nitrogen (SUN), liver glycogen, Superoxide Dismutase (SOD) and glutathione peroxidase (GSH-Px) contents. The results suggested that GL-PS could extend the swimming time to exhaustion of the mice, as well as increase the liver glycogen, SOD and GSH-Px contents, while decrease the blood lactic acid and serum urea nitrogen contents. Therefore, GL-PS had anti-fatigue effects.
  Zhiwei Liang , Xudong Ma , Fang Fang and Songhao Zhu
  In order to overcome the difficulty of a mobile robot to perform localization only with its onboard sensors, this study presents a probabilistic algorithm Monte Carlo Localization (MCL) to solve the problem of mobile robot localization in a hybrid robot and camera network in real time. On one hand, the robot does perform localization depending on its laser sensor using Monte Carlo method. On the other hand, environment cameras can detect the robot in their field of view during robot localization. According to a built environment camera model, MCL method extended to update robot’s belief whichever information (positive or negative) attained from environmental camera sensors. Meanwhile, all the parameters of each environmental camera are unknown in advance and need be calibrated independently by robot. Once calibrated, the positive and negative detection models can be built up according to the parameters of environmental cameras. A further experiment, obtained with the real robot in an indoor office environment, illustrates it has drastic improvement in global localization speed and accuracy using our algorithm.
  Fang Fang , Ma Xudong and Dai Xianzhong
  A novel extended particle algorithm is proposed aiming at solving the unmodeled motion problem, which is inextricable merely using conventional MCL. KLD sampling is utilized to measure the approximation error by Kullback-Leibler distance between the true distribution and its sampled representation by generating a dynamically sized set of samples according to the exact posterior being estimated. At the same time, the over-convergence and uniformity validations are introduced to verify correspondence between sample distribution and sensor information for timely re-sampling which highly saves computational resource and enhances localization efficiency. A further experiment, obtained with a real robot in an indoor environment, illustrates the favorable performance of this approach.
  Yan Fei-Wei , Fang Fang , Jin Hong-Mei and Jin Ye
  Tartary Buckwheat Extracts (TBE) has been reported to be a potential antioxidant that may affect health and exercise performance. The objective of this study was to examine the effects of TBE supplementation on oxidative stress induced by acute exhaustive exercise in rats. Male Sprague-Dawley rats were randomly divided into four groups, each consisting of ten rats. The first group designated as control group was administered with distilled water by gavage every day for 28 days. The other three groups designated as TBE supplementation groups were administered with TBE of 60, 120 and 240 mg kg-1 body weight (b.wt.), respectively, by gavage every day for 28 days. After acute exhaustive exercise, the antioxidant enzyme activities and malondialdehyde (MDA) levels were determined. The data showed that TBE at the dose 60, 120 and 240 mg kg-1 b.wt. increased Super Oxide Dismutase (SOD) activities in liver tissue of rats significantly by 28.13, 34.65 and 45.95%, respectively, increased catalase (CAT) activities significantly by 29.49, 41.63 and 59.30%, respectively and decreased MDA levels significantly by 31.21, 51.12 and 45.68%, respectively. TBE at the dose 120 and 240 mg kg-1 b.wt. increased glutathione peroxidase (GPx) activities significantly by 21.33 and 28.22%, respectively. TBE at the dose 240 mg kg-1 b.wt. increased Glutathione Reductase (GR) activities significantly by 31.58%. These results suggested that TBE supplementation has a protective effect on oxidative stress induced by acute exhaustive exercise.
  Yimin Ren , Hong Wei Xu , Fleur Davey , Margaret Taylor , Jim Aiton , Peter Coote , Fang Fang , Jun Yao , Doris Chen , John Xi Chen , Shi Du Yan and Frank J. Gunn-Moore
  Alzheimer patients have increased levels of both the 42 amyloid-β-peptide (Aβ) and the amyloid binding alcohol dehydrogenase (ABAD), which is an intracellular binding site for Aβ. The overexpression of Aβ and ABAD in transgenic mice has shown that the binding of Aβ to ABAD results in amplified neuronal stress and impairment of learning and memory. From a proteomic analysis of the brains from these animals, we have identified for the first time that the protein endophilin I increases in Alzheimer diseased brain. The increase in endophilin I levels in neurons is linked to an increase in the activation of the stress kinase c-Jun N-terminal kinase with the subsequent death of the neurons. We also demonstrate in living animals that the expression level of endophilin I is an indicator for the interaction of ABAD and Aβ as its expression levels return to normal if this interaction is perturbed. Therefore this identifies endophilin I as a new indicator of the progression of Alzheimer disease.
  Meng Ding , Bin Yao , Dongxu Zhao , Fang Fang , Dezhen Shen and Zhenzhong Zhang
  As-doped sub-micron ZnO rods were realized by a simple thermal diffusion process using a GaAs wafer as an arsenic resource. The surface of the sub-micron ZnO rods became rough and the morphology of As-doped sub-micron ZnO rods changed markedly with increasing diffusion temperature. From the results of energy-dispersive X-ray spectroscopy, X-ray diffraction and photoluminescence, arsenic elements were confirmed to be introduced into the sub-micron ZnO rods. The acceptor ionization energy was deduced to be about 110 meV based on the temperature-dependent PL spectra.
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