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Articles by Fahrul Huyop
Total Records ( 6 ) for Fahrul Huyop
  Siti Nurmadihah Ismail , Aishah Mohd Taha , Ng Hong Jing , Roswanira Ab. Wahab , Aidil Abdul Hamid , Rolando V. Pakingking Jr. and Fahrul Huyop
  A bacterial strain tentatively identified as Pseudomonas sp. R1 was isolated from a paddy (rice) field that could degrade monochloroacetic acid (MCA) for concentrations ranging from 5 to 40 mM. Quantitative agreement between the amount of MCA introduced and chloride released was also found. MCA dehalogenase activity in this strain was found to be inducible. Cell-free extracts displayed dehalogenating activity with specific halogenated organic compound with no activity on dichloropropionic acid or monochloropropionic acid. The estimated Km values for MCA was 0.14 mM. The optimal pH range for MCA dehalogenase activity (between pH 6.5 and 8.0), whereas the thermal stability profile stable up to 50 °C. The results of our current study demonstrated the potential use of Pseudomonas sp. R1 as suitable biological agent for biodegradation of MCA in contaminated agricultural area.
  Tan Yea Yusn and Fahrul Huyop
  3-Chloropropionic acid (3CP) in its carboxylate ionic form is a synthetic compound found in herbicide. The biodegradability of 3CP is not well documented but a microbe that has the ability to utilise 3CP as sole carbon and energy source has been isolated. The dehalogenase gene (deh) cloned from Rhodococcus sp. HJ1 could be used as a selection marker gene for vector in E. coli. Halogenated compound, especially 3CP inhibit the growth of some microorganisms. In current investigation, a 4 kb EcoR1 fragment of genomic DNA from Rhodococcus sp. HJ1 was cloned into pUC18 plasmid and transformed into an E. coli JM109 conferred 3CP resistance on them. Therefore, E. coli transformed with vector marked with deh could be easily selected on plates containing 3CP. The E. coli JM109 transformed with pTY096 (deh+) weakly expressed the deh gene as shown from its slow growth with cells doubling time of 22 h with minimal amount of chloride ion released in the growth medium.
  Farhad Taghipour , Narges Janalizadeh , Maryam Eshrati , Taraneh Hassanzadeh and Fahrul Huyop
  An applicable in vitro callus initiation and shoot regeneration of commercial sugar beet breeding lines was investigated. Procedures are described for producing different types of callus and competent callus formation able to produce shoots. Leaf, cotyledon and hypocotyl explants taken from in vitro grown seedlings were subjected to Murashige and Skoog (MS) medium containing different combination of plant growth regulators. The leaf explants of genotype 436 showed the highest competent callus production able to form shoot when 0.1 mg L-1 α-Naphthaleneacetic (NAA) acid was used in combination with 1 mg L-1 thidiazuron (TDZ). Hypocotyl explants of IC genotype showed the highest callus formation when 0.1 mg L-1 NAA was combined with 0.3 mg L-1 6-Benzylaminopurine (BAP). Cotyledon explants of IC genotype showed the high callus production when 0.3 mg L-1 TDZ was combined with 0.1 mg L-1 NAA. The result evaluation demonstrated that cotyledon explants of genotype 436 produced more callus than IC genotype which able to produce shoot and the best callus induction medium was MS medium in combination with 0.1 mg L-1 NAA and 1 mg L-1 TDZ. In conclusion, TDZ was more effective cytokinin than BAP and NAA was more efficient auxin than both Indol-3-Acetic Acid (IAA) and 2, 4-Dichlorophenoxyacetic acid (2, 4-D).
  Kavitah Ganasan and Fahrul Huyop
  The objective of the current study was to establish an efficient and reproducible in vitro plant regeneration protocol using cotyledonary explant for Citrullus lanatus cv. Round Dragon. To achieve optimal conditions for adventitious shoot induction, cotyledon explants of 5-day-old seedlings, 7-day-old seedlings and 9-day-old seedlings were tested for regeneration potential on Murashige and Skoog (MS) media supplemented with 2.3 mg L-1 BAP. Results showed that high frequency of in vitro adventitious shoot regeneration was induced from the proximal region of 5-day-old seedlings (93%) with 19.80±0.99 shoots per responding explant after 6 weeks. Adventitious shoots induced from 5-day-old seedlings after 6 weeks were transferred to MS shoot regeneration medium without plant growth regulator for shoot elongation for 4 weeks. The influence of various concentrations of IBA, IAA and NAA on root initiation was examined on half-strength and full-strength of MS rooting medium. The best response for root initiation was obtained from the microshoots grown in full-strength MS rooting medium compared to the half-strength MS rooting medium. Furthermore, IBA was more efficient in promoting root induction than IAA and NAA, resulting in a higher rate of root initiation (100%) at the concentration of 0.1 mg L-1 IBA. Therefore, elongated shoots were rooted in MS medium supplemented with 0.1 mg L-1 IBA for 3 weeks. Rooted plantlets were acclimatized successfully under ex vitro conditions.
  Mohammed Faraj Edbeib , Roswanira Abdul Wahab and Fahrul Huyop
  Halogenated Organic Compounds (HOC) are major pollutants in the environment due to their wide usage as biocides in the agricultural industry. Hence, it is important that safe methods to eliminating such compounds are developed. Consequently, the goal of this study is to isolate and identify bacterial species capable of utilizing 2,2 Dichloropropionic acid (2,2-DCP). Using enrichment culture, a bacterium strain designated as M×2 was isolated from contaminated seawater located at desaru beach, Malaysia. The isolate grew best at 20 mM of 2,2-DCP minimal media with doubling time of 13.9 h. More than 0.59 mmol/L chloride ions were detected in the growth medium. Morphological and biochemical characteristics of M×2 were closely matched to those of Achromobacter sp. and the 16S rDNA sequence (GenBank accession number KP336491) 98% identical to that of several species of the genus Achromobacter. This is one of the first studies detailing the ability of Achromobacter sp. to utilize 2,2-DCP as the sole source of carbon and energy.
  Ng Hong Jing , Roswanira Ab. Wahab , Aishah Mohd Taha , Noor Aini Abdul Rashid and Fahrul Huyop
  The main aim of the present study is to further characterize a new dehalogenase enzyme found in the crude extracts from Rhodococcus sp. The ability of the enzyme to catalyze the dehalogenation of various halogen-substituted organic acids was investigated and the highest activity was found with 3-chloropropionic acid as a sole carbon source in the growth medium. The enzyme followed Michaelis-Menten kinetics and the Km for 3-chloropropionic acid was 0.2 mM. Maximum activity was found at pH 7.6 at 30°C. The enzyme activity in the cell-free extract was unaffected by diaminoethane tetraacetic acid (EDTA), dithiothreitol (DTT) or by Mn and Zn ions but was reduced by HgCl2 (70%) and Pb(NO3)2 (80%). The enzyme removed the chlorine atom present on a number of 3- and 4-carbon alkanoic acids if the halogen was on the β-position.
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