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Articles by Fahim Ullah
Total Records ( 2 ) for Fahim Ullah
  Lubna Hassan and Fahim Ullah
  Background and Objective: Staphylococci strains causing bovine mastitis, has produced serious therapeutic problems. However, the underlying mechanisms are poorly understood and it is necessary to develop new effective method for accurate diagnosis. Resazurin micro-plate method was used for the diagnosis of bovine mastitis caused by staphylococci. The objective of this study was to discuss about the method of resazurin micro-plate for the diagnosing of bovine mastitis which caused by staphylococci. Methodology: While for testing the infected mastitis milk samples were inoculated in the selection of the Staphylococcus-mBP medium with 96-well micro-plate and pure cultures of bacteria and after 2 h of the incubation the resazurin indicator was added to that solution up to 0.4 g L–1 and then continued the cultivation for 10 h, depending on whether the medium color changes of bacterial growth. For statistical analysis chi-square test was performed using SPSS Software version 22 (IBM SPSS Statistics for Windows, Armonk, NY, USA: IBM Corp.). Results: The results from the bacterial pure culture plate showed that the resazurin micro-plate indicated the growth of S. aureus i.e., S. epidermidis, S. chromogens, S. haemolyticus, S. simulans , S. xylosus and E. coli did not appear on the culture plates. Further from the investigated results showed that the color changing in the medium was due to the staphylococcal concentration and time incubation. However, the results of the infection milk samples showed that the resazurin micro-plate had a detection sensitivity of 1×103 CFU mL–1 and in 12 h the diagnosis could be obtained. Conclusion: Furthermore from the results of the mastitis milk samples, the positive culture plate showed that the resazurin micro-diagnosis of S. aureus-positive and negative milk sample at the rate of 98.3% (n = 60) and 95.0% (n = 40) respectively. Similarly, from all the investigated results it is concluded that the resazurin micro-plate can replace the conventional method for rapid diagnosis of Staphylococcus milk samples.
  Fahim Ullah , Mansoor Khan Khattak , Min Kang , Ninghui Li , Jun Yang and Xingsheng Wang
  Background and Objectives: In this study, the numerical simulation of the steady-state thermal performance of flat plate solar collector with double glass cover, physical model, validation and its grid independence test were performed with the finite volume method. Moreover, through comparison with the conventional plate heat collector, the effects of ambient temperature, the inlet water temperature, solar radiated intensity and lower glass cover position on thermal performance of the heat collector were investigated. Materials and Methods: For the simulation of FPSC with double glass covers different physical model, geometric and boundary condition were used and also regression analysis was applied on the data. Results: From the investigated results, it was cleared that, when the ambient temperature is below 18°C, the collector with double glass covers shows advantages, while when the ambient temperature is -10°C, the instantaneous efficiency of the flat plate solar collector with double glass covers was noted 0.26 higher than that of the conventional plate heat collector. Similarly, increase in the inlet water temperature and solar radiate intensity both results in an increase in the temperature inside the collector, which enhances the insulation effect of the double glass covers. Conclusion: When the inlet water temperature was 50°C, the instantaneous efficiency difference between the flat-plate solar collector with the double glass covers and the conventional one increases to 0.27. Furthermore, concluded from the results of the experiment that, when the lower glass cover is located 18 mm over the absorber plate and the solar radiated intensity is to be 1000 W m–2, the instantaneous efficiency of the flat-plate solar collector with the double glass covers can reach up to 0.678.
 
 
 
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