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Articles by Faez Firdaus Jesse Abdullah
Total Records ( 2 ) for Faez Firdaus Jesse Abdullah
  Faez Firdaus Jesse Abdullah , Abdinasir Yusuf Osman , Lawan Adamu , Zunita Zakaria , Rasedee Abdullah , Mohd Zamri-Saad and Abdul Aziz Saharee
  Acute Phase Protein (APP) investigations of serum or plasma following natural or experimental infection frequently reveal substantial alterations in the APPs, several of which are of veterinary importance in the assessment of herd health. The present study of the experimental nature was conducted to evaluate the acute phase protein responses; haptoglobin, Serum Amyloid A (SAA) and serum albumin in relation to infection with Pasteurella multocida type B and its immunogens; lipopolysaccharide (LPS) and Outer Membrane Protein (OMP) in calves. Eight clinically healthy, non-pregnant and non-lactating Brangus cross calves weighing 150±50 kg were used in this study. The calves (n = 8) were divided into 4 groups of 2 calves in each group. The control group was inoculated with sterile Phosphate Buffered Saline (PBS) whereas group 2 were inoculated with wild-type P. multocida type B:2 and group 3 and 4 were inoculated with LPS and OMP respectively. Blood samples were collected via jugular vein-puncture at 3 h intervals for APPs analysis. APPs were quantified by commercially available ELISA methods. Moribund animals were euthanized while the surviving animals were killed after 48 h. The results revealed that there were statistically significant differences (p<0.05) between APPs concentrations throughout the experimental period in challenged groups compared to control groups. Over 7-fold increase was observed in Hp concentrations with mean maximum levels of 1.316±0.558 ng mL-1, 1.521±0.687 ng mL-1 after experimentally induced with OMP and LPS respectively. SAA increased less than 3-fold with mean levels of 2.187±0.880 ng mL-1, 2.421±0.432 ng mL-1, 2.657±0.099 ng mL-1 in almost all challenged groups; Pasteurella Multocida, OMP and LPS respectively. In contrast, for the negative APP, the albumin levels of groups LPS and OMP did not show significant difference (p>0.05) with mean levels of 32.677±1.556 and 36.185±2.239 U L-1, respectively. While P. multocida group (22.193±2.727 U L-1) showed statistically significant difference (p<0.05) than the negative control group (34.233±6.900 U L-1). In conclusion, the findings of this study indicated that APPs; SAA and haptoglobin are sensitive biomarkers to explore host response in relation to Haemorrhagic Septicaemia infections in clinical settings.
  Faez Firdaus Jesse Abdullah , Lawan Adamu , Abdinasir Yusuf Osman , Mohd Zamri Saad , Zunita Zakaria , Rasedee Abdullah and Abdul Aziz Saharee
  Haemorrhagic Septicaemia (HS) is a killer disease of cattle and buffalo of economic importance in Asia and Africa. There is insufficient information on the responses of Balb c mice as animal model in respect of immunogens and Acute Phase Proteins (APP) profiles. Therefore, the present study aims to evaluate the acute phase protein profiles in mice associated with the infection of Pasteurella multocida type B and the bacterial lipopolysaccharide and outer membrane protein immunogens. Two hundred healthy Balb/c mice of 8-10 weeks old were used in this study. They were divided into four equal groups of 50 mice each. Mice of group 1 were inoculated intra-peritoneal with 1.0 mL sterile Phosphate Buffered Saline (PBS) pH 7, group 2 were inoculated with 1.0 mL of 109 colony forming unit (cfu) of P. multocida B: 2. Mice of groups 3 and 4 were inoculated intra-peritoneal with 1.0 mL of LPS and 1.0 mL of OMP, respectively. Acute phase proteins analysis were done using two sites Enzyme Linked Immunoassay (ELISA) highly sensitive test kits. The data was analyzed using SPSS. Haptoglobin concentration increased significantly in group 3 and 4 (p<0.05) following inoculation with immunogens compared to control group. Mice in group 3 and 4 showed significantly (p<0.000) 3 times higher concentrations of SAA and significantly (p<0.037) 1.3 times increased concentrations of SAA, respectively compared to the control group. There was no significant changes in the concentrations of fibrinogen in group 2 (p = 0.177), group 3 (p = 0.088) and group 4 (p = 0.359). C-reactive protein in groups 2 and 3 showed significantly (p<0.05) higher levels than the control group. Albumin showed significant increase (p<0.05) in group 2 compared to the control group. There were significant changes in the concentrations of acute phase proteins and clinical responses post inoculation with immunogens indicating adverse pro-inflammatory reactions in mice in the present study.
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