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Articles by F.M. El-Moghazy
Total Records ( 3 ) for F.M. El-Moghazy
  W.M. Ahmed , A.R. Abd El-Hameed and F.M. El-Moghazy
  This study was designed to associate Blood Lead Concentration(BLC), reproductive disorders and oxidant/antioxidant status in female buffaloes reared besides high ways. Animals were clinically examined and blood samples were collected from 30 non pregnant female buffaloes for assaying of lead and some oxidant/antioxidant values. According to BLC, animals were divided into two groups. The high BLC group showed high incidence of reproductive disorders in form of inactive ovaries, delayed puberty, endometritis, repeat breeding, mastitis, persistent corpora lutea and abortion. Malondialdehyde (MDA) and Nitric Oxide (NO) values increased, while, Total Antioxidant Activity (TAA), Superoxide Dismutase (SOD), Glutathione Reduced (GR) and Selenium (Se) values decreased in buffaloes of high BLC. It was concluded that there is a tight relationship between blood lead concentration, reproductive disorders and oxidant/ antioxidant imbalance in buffaloes.
  H.A. Shalaby and F.M. El-Moghazy
  Since the integrity of Toxocara vitulorum cuticle is essential for the nutritive and protective functions, light and scanning electron microscopic studies were undertaken to assess, for the first time, whether the Nigella sativa oil (NSO) had any effect on the cuticle of adult T. vitulorum following incubation in vitro. Differences in response to NSO action were observed, depending on the used concentration. After 24 h incubation with 0.5 mg mL-1 NSO, the cuticle of the anterior end of worms appeared to be slightly more swollen than normal. This swelling became pronounced and so severe, with lips showed wrinkled cuticular surface and deformed sensory papillae on increasing the concentration to 1.0 mg mL-1. With the higher concentration of 1.5 mg mL-1, extensive and severe disorganization of the cuticle and body musculature was observed. Structural alterations in the cuticle as observed in the present investigation were thus, clear indication of nematocidal activity of NSO that could offer a suitable and cheaper alternative for the more expensive anthelmintics.
  H.A. Shalaby , F.M. El-Moghazy and F.A.M. Khalil
  In this study, specificity of snail feet and hepatopancreases antigens in antibody detection of their trematode parasite was evaluated via Western blot technique. Snails, of two different families, antigens; Lymnaea cailliaudi as intermediate host of Fasciola gigantica and Biomphalaria alexandrina as intermediate host of Paramphistomum microbothrium, were evaluated in detection of IgG antibodies against their trematode parasites after preparation of the required Hyper-Immune Sera (HIS) in rabbits. The results revealed higher specificity of snail feet in antibody detection than hepatopancreases antigens. Where, three of sex polypeptides of L. cailliaudi feet antigen; identified by F. gigantica HIS, showed specific positive reactivity. These polypeptides were at molecular weights of 59, 57 and 52 kDa. While, one of sex polypeptides of L. cailliaudi hepatopancreases antigen; identified by F. gigantica HIS, at molecular weight of 57 kDa was specific. Similarly, two polypeptides of B. alexandrina feet antigen; at molecular weights of 54 and 45 kDa, showed specific reactivity toward anti-paramphistomum antibodies. At a time, no specific reactivity had been shown by the antigenically active polypeptides of B. alexandrina hepatopancreases antigen. Owing to its cross-reactivity with incompatible trematode parasite, snail antigen can be used as a general starting antigen for immunodiagnosis of trematode infections.
 
 
 
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