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MBCD and Cholesterol-Loaded-Cyclodextrin (CLC) were
examined for their abilities to increase the cryosurvival of C57BL/6 mouse
sperm, the main strain of genetically engineered mice. The intactness
of acrosome and motility of frozen/thawed spermatozoa were used to monitor
cryosurvival. In this experimental study, male mice were randomly divided
in 6 groups: control 1, experimental 1, experimental 2, control 2, experimental
3 and experimental 4. In experimental groups 1 and 2 spermatozoa were
exposed to 0.75 and 1 mM MBCD and in experimental groups 3 and 4 were
exposed to two different concentrations of CLC (1 and 2 mg mL-1)
over a period of 1 h and were subsequently cryopreserved. Spermatozoa
in control 1 group were frozen without any exposure to CLC or MBCD and
in control 2 (vehicle), sperms were incubated with 4 mM MBCD. The post-thaw
sperms were evaluated for their motility and acrosomal status. The values
of the intact acrosome and motility increased significantly with concentration
of CLC compared to controls and MBCD experimental groups (p<0.05).
These results indicate that cryosurvival of C57BL/6 mouse spermatozoa
is enhanced by exposure to MBCD which loaded with cholesterol (CLC) before
freezing and MBCD alone can not protect sperm from freeze-thaw damage
efficiently compare to CLC.
