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Articles by Euis Reni Yuslianti
Total Records ( 5 ) for Euis Reni Yuslianti
  E.R. Yuslianti , B.M. Bachtiar , D.F. Suniarti , A.B. Sutjiatmo , Euis Reni Yuslianti , Boy M. Bachtiar , Dewi F. Suniarti and Afifah B. Sutjiatmo
  Rambutan honey often used for topical treatment sores in the oral, because it has a good taste and fragrant. The use of rambutan honey empirically efficacious in wound healing has been scientifically proven yet, as a product or stimulant that serves to accelerate oral mucosa wound healing is still very limited. This study aimed to analyze topical Rambutan Honey Pharmaceuticals Grades (RHPG) in influencing wound closure and stimulation of the fibroblasts proliferation in the oral mucosa wound healing in vivo. The research method was experimental laboratory. Rats (n = 16/gp) were divided into negative control, positive control φ4±2 mm wound, 1 mL RHPG wound and 0.1 mL ascorbic acid. Oral mucosal wound (palatal region) were observed on day 0, 3, 7 and 14 administered topically twice. The data analysis are using the ANOVA and post hoc Tukey with p<0.05. The results show the RHPG effect on the wound width significantly at days 0 (p = 0.002), 3rd (p = 0.005) and the 7th (p = 0.009) and to the amount of fibroblasts significantly at days 0, 3rd and 7th with p = 0.001, fibroblasts cell amount per high power fields mean 101.75±8.99 (NC), 69±12.65 (PC), 83.25±25.83 (RHPG), 86.5±5.84 (AA), respectively. In conclusion, that topical administration of pharmaceuticals standard rambutan honeys effect to oral mucosa wound closured and to fibroblasts proliferation in vivo.
  Iis Inayati Rachmat , Euis Reni Yuslianti , Githa F. Permatasari and Teja Koswara
  Background and Objective: Diabetes mellitus is a chronic metabolic disorder characterized by inappropriate hyperglycemia due to lack of or resistance to insulin and free radicals involved. Honey is known to have an antioxidant and antihyperglycemic effect in community. However, the mechanisms of rambutan honey action as antihyperglycemic have poorly understood. The objectives of this study were to investigated the effect of rambutan honey toward to decrease blood glucose level and to histopathology-changing aspects in the pancreas of diabetic Wistar rat. Materials and Methods: The research methods were an experimental laboratory. The samples (n = 30) were divided into six groups, the negative control which normal blood glucose (NC), Positive Control (PC), the group of rambutan honey 0.5, 1 and 2 g kg–1 b.wt. and glibenclamide 0.065 mg/200 g b.wt. Diabetes was induced in rats by an intraperitoneal injection of alloxan. Rambutan honey was orally administered to normal and diabetic rats for a period of 21 days. Blood glucose level was observed on day 0, 7th, 14th and 21st and the last day of the pancreas histopathologic examination stained with haematoxylin-eosin. Data were statistically analyzed using ANOVA and paired t-test. Results: Rambutan honey 0.5 g kg–1 b.wt. decrease blood glucose level significantly on day 21 (p = 0.047) with blood glucose level means of 195.2±19.83 mg dL–1 (PC), 127.6±25.46 mg dL–1 (0.5 g kg–1 b.wt.), 187.6±54.83 mg dL–1 (1 g kg–1 b.wt.) and 243.6±77.62 mg dL–1 (2 g kg–1 b.wt.), respectively and had no significant differences compared with glibenclamide. Conclusion: Rambutan honey exhibits antihyperglycemic activity in alloxan-induced diabetic rats. Thus our finding provides clear evidences that rambutan honey have a potential as an adjuvant along with antidiabetics agent treatment. However, such effects need to be confirmed on human in clinical condition.
  Henny Juliastuti , Ardela Tri Novianti , Bayan Akid Fatawi and Euis Reni Yuslianti
  Background and Objective: Breadfruit leaf (Artocarpus altilis) can be used to cure several diseases such as renal disease, high blood pressure, liver, diabetes, asthma, toothache, skin infection and cancer in Indonesian community. Liver disease including hepatitis and hepatotoxicity require proper management so they may heal faster. This study was aimed to examine the hepatoprotective effect of ethanol extract of Indonesian breadfruit leaf in carbon tetrachloride-induced liver injury Wistar rats by measuring Serum Glutamic Pyruvic Transaminase (SGPT) and malondialdehyde (MDA) levels. Materials and Methods: Wistar rats were divided into negative control group, positive control group and three treatment groups. Ethanol-based breadfruit leaf extract were formulated in doses of 125 mg kg–1 b.wt., (T1), 250 mg kg–1 b.wt., (T2) and 500 mg kg–1 b.wt., (T3) applied per oral for 7 days. Positive control and treatment groups were induced by 1 mL kg–1 b.wt., dose of carbon tetrachloride intraperitoneal on day 8. Data were statistically analyzed using one-way ANOVA and post hoc test. Results: The research revealed that all groups treated with breadfruit leaf have the lower of MDA and SGPT levels (p<0.05) when compared to the positive control. Ethanol-based breadfruit leaf extraction doses of 250 and 500 mg kg–1 b.wt., per oral given for 7 days before induced by 1 mL kg–1 b.wt., dose of carbon tetrachloride showed significance in decreased MDA levels (p = 0.017) however dose of 500 mg kg–1 b.wt., showed the most effective to decreased SGPT levels (p = 0.000). Conclusion: It is concluded that ethanol-based breadfruit leaf extract had effect on liver protection by decreased levels of MDA and SGPT.
  Euis Reni Yuslianti , Boy M. Bachtiar , Dewi F. Suniarti and Afifah B. Sutjiatmo
  Rambutan honey has been reported to be effective therapeutic in wound healing, may be due to it antioxidant activity, but research of rambutan honey antioxidant activity is still very limited. The purpose of this study is to determine rambutan honey free radicals scavenging activity in vitro and lipid peroxidation inhibition of oral mucosa wound tissue in vivo. The research method was experimental laboratory. The sample used for DPPH test was pure isolated rambutan honey with concentration of 0.5, 1, 2, 4 and 8 mg mL–1. Experimental animals used were 64 male Wistar rats. All rats were injured except for the Negative Control group (NC), using the 4 mm-diameter punch biopsy on the palate and then divided into groups of Positive Control (PC), a group with a topical application isolate of Rambutan (RH) 1 mL and group with topical application of Vitamin C (VC) 0.1 mL, then sacrificed at day 0, 3, 7 and 14 and measured lipid peroxidation inhibition of wound tissue with TBARs method. Data were analyzed statistically, using Kruskal Wallis and Mann-Whitney post hoc. The results showed rambutan honey 1 mg mL–1 have DPPH inhibition of 45.3% and the lipid peroxidation inhibition activity in oral mucosa wound tissue significantly on day 3 (p = 0.028) and 14th (p = 0.037). The conclusions were rambutan honey has antioxidant activity in vitro and in vivo that these natural substances have the potential to be used as an antioxidant drugs in human oral mucosal healing.
  Eka Noneng Nawangsih , Sayu Putu Yuni Paryati , Yoga L. Baklaes and Euis Reni Yuslianti
  Background and Objective: Munghurt is a probiotic made from green beans. Mung beans possess enzymes, nutrients, antioxidants and low fats essential for health. Green beans contain higher carbohydrate than cow milk better to growth Lactobacillus acidophilus. The objective of this research was to determine effect of munghurt Lactobacillus acidophilus to blood glucose levels in diabetic rats induced alloxan. Materials and Methods: The samples (n=25) were divided into five groups, negative control (Negative); positive control (Positive); glibenclamide-treated (Glibenclamide); munghurt Lactobacillus acidophilus-treated (Munghurt); glibenclamide and munghurt Lactobacillus acidophilus combination-treated (Glibenclamide+Munghurt). Diabetes rat was induced by an intraperitoneal injection of alloxan. Munghurt Lactobacillus acidophilus was orally administered to normal and diabetic rats for a period of 4 weeks. Blood glucose level was observed on 0 and 28 days. Data were statistically analyzed using Kruskall Wallis and Mann Whitney test and further by one way ANOVA and Tukey post hoc test. Results: Munghurt Lactobacillus acidophilus decreased blood glucose level significantly on 28 days (p = 0.001) with blood glucose level mean of 87.32±5.15 mg dL–1 (negative), 233±17.323 mg dL–1 (positive), 117.36±8.153 mg dL–1 (munghurt), 105.46±3,756 mg dL–1 (glibenclamide) and 105±4.617 mg dL–1 (munghurt+glibenclamide) respectively. Munghurt Lactobacillus acidophilus combined with glibenclamide had best results at lowering blood glucose in diabetic rats (p<0.016). Conclusion: Munghurt Lactobacillus acidophilus had the effect of the decreasing blood glucose levels and there were a complementary mechanisms between munghurt Lactobacillus acidophilus and glibenclamide in lowering blood glucose level in alloxan-induced diabetic rats. However, such effects need to be confirmed on human in clinical condition.
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