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Articles by Estefan Miranda-Miranda
Total Records ( 2 ) for Estefan Miranda-Miranda
  Raquel Cossio-Bayugar , Estefan Miranda-Miranda , Alejandrina Ortiz-Najera and Salvador Neri-Orantes
  This study was made to assess SDS-PAGE monooxygenases zymograms in the presence of synthetic monooxygenase enzyme substrates, in order to establish a reliable methodology to measure monooxygenase activity levels on acaricide resistant ticks and finding a probable association between monooxygenase enzymatic levels and B. microplus acaricide resistance. Monooxygenase activity was found in the zymograms of the analyzed ticks at three polypeptidic bands of 147, 125 and 98 kDa, densitometric measurements of monooxygenases zymograms at these specific enzymatic bands showed a statistically significant monooxygenase activity increase (p<0.001 unpaired t-test) on Pyrethroid resistant ticks when compared to the same bands in the acaricide susceptible reference ticks. Present results showed that monooxygenase zymograms combined with densitographic analysis, are useful as an acaricide resistance detection test and suggest a linkage between increased monooxygenase activities and Pyrethroid acaricide resistance in B. microplus.
  Raquel Cossio-Bayugar , Estefan Miranda-Miranda , Alejandrina Ortiz-Najera , Salvador Neri-Orantes and Francisco Olvera-Valencia
  This study was done with the objective of establishing a relationship between cytochrome P-450 gene expression and acaricide resistance levels in Mexican isolates of R. microplus, for that purpose the cytochrome P-450 expression levels were measured by real time PCR quantification on acaricide sensitive and resistant strains of ticks, as well as on acaricide resistant an sensitive ticks isolated from cattle at the southeastern Mexican state of Chiapas and the northern Mexican State of Tamaulipas. Acaricide susceptible ticks were used as comparison standard P-450 expression level and adjusted as a baseline of 1 Relative Expression Units (REU). A statistically significant (p<0.0001 unpaired t-test) 3.47±0.302 REU overall increase on the P-450 gene expression level was detected on pyrethroid resistant ticks, we also found a statistically significant (p<0.0001 unpaired t-test) P-450 expression decrement of 0.335±0.054 REU in multiple acaricide resistant ticks, no significant difference on cytochrome P-450 expression level was detected on the organophosphate acaricide resistant strain. Our results suggest that cytochrome P-450 monooxygenases play a role on at least some types of acaricide resistance phenomenon and the assessment of the data reveals a multifactorial causative for organophosphate and pyrethroid resistant R. microplus.
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