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Articles by Esra Seker
Total Records ( 3 ) for Esra Seker
  Esra Seker , Yahya Kuyucuoglu and Selahattin Konak
  The aim of this study was to examine the bacterial flora of nasal cavity in apparently healthy and unhealthy Holstein cattle and determine the antimicrobial susceptibilities of Pasteurella multocida and Mannheimia haemolytica strains isolated from samples. Nasal swab samples were collected from 100 Holstein cattle, 70 of these were apparently healthy and 30 were unhealthy with the following respiratory signs: nasal discharge, coughing and dyspnoea. A total of 220 bacterial isolates were detected on Columbia blood agar plates, 102 (46.4%) of these were recovered from healthy and 118 (53.6%) from unhealthy Holstein cattle. The most frequently isolated species from the nasal cavity of the healthy animals sampled were Staphylococcus epidermidis (32.9%) and S. aureus (24.3%), while the most prevalent species in unhealthy cattle were Pseudomonas aeruginosa (40.0%), P. multocida (40.0%) and M. haemolytica (100.0%). According to antibiotic susceptibility test results, enrofloxacin (95.0%) was the most effective antibiotic on P. multocida isolates, while 95.0% of M. haemolytica isolates were susceptible to gentamicin. Among the P. multocida isolates the highest resistance was found against to kanamycin (70.0%), while erythromycin resistance were detected as 90.0% for M. haemolytica isolates.
  Erhan Ozenc , Esra Seker and Nurhan Dogan
  The aim of this study was to determine the correlation between pH values for the uterine horns and uterine bacterial flora during phases of sexual cycle, pregnancy and cases of clinical metritis in the cow. Post-mortem pH values for the uterine horns of 145 cows were measured using a digital pH meter with a flexible probe. In addition, stage of the sexual cycle of the cows was evaluated by external examination and microbiology and ultrasonography. The mean pH values for Escherichia coli and Staphylococcus aureus+E. coli growth within the uterine horns were detected to be higher than those with Micrococcus sp. and Coagulase Negative Staphylococci (CNS)+Candida sp. growth (p<0.05). As the mean uterine pH value of microbial growth at estrous cycle was determined to be significantly higher than those without microbial growth (p<0.05), the mean pH value for uterine horns with microbial growth were determined to be higher than without microbial growth when phases of sexual cycle were collectively evaluated (p<0.05). In addition, mean pH values during pregnancy in the uterus with microbial growth were detected to be significantly higher than those of without microbial growth (p<0.05). However, the mean pH value of uterine secretions was determined as 7.25±0.44 in cases of clinical metritis. In conclusion, selection of intrauterine drugs and phases of drug use in cows were deemed to be designed by considering the uterine bacterial flora and phases of sexual cycle.
  Erhan OZENC , Mehmet Rifat VURAL , Esra SEKER and Mehmet UCAR

Subclinical mastitis in Anatolian buffaloes (n = 71) in Afyon, Turkey was evaluated using 1637 milk samples collected monthly for 9 months. Tests applied to milk samples included the California Mastitis Test (CMT), somatic cell count (SCC), and bacteriological examination.

The threshold limit for SCC was found as 130 x 103 cells/ml. Anatolian buffaloes are infected above this level and they are likely to be uninfected below this level. The highest rates of intramammary infection (IMI) per quarter (36.5%) and animal (69.1%) were encountered during the highest rainfall month of May. Candida spp., coagulase-negative staphylococcus (CNS) and Staphylococcus aureus and mixed infections were isolated at the rate of 41.91%, 20.59%, and 16.91% of quarters, respectively. Quarters infected with S. aureus (9.7%) were associated with CMT scores >0 and elevated SCC. No significant increase in CMT scores >0 and elevated SCC were observed during the period in which the incidence of Candida spp. was high. However, CMT (-) milk samples were determined to display higher SCC in autumn months when compared to other seasons. The microbial isolation rates in CMT (-) and CMT (+) quarters during the first 4 months of lactation were determined to be higher in comparison to other stages of lactation. The most sensitive antibiotic was amoxycillin+clavulonic acid.

As it has low numbers of infectious agents, bubaline milk is important for human welfare. However, higher infection rates were observed during rainy periods, during the first 4 months of lactation, after the 5th lactation, in late spring and early summer calvers, and in machine milked animals. In addition to SCC and CMT results, bacteriological examination could be carried out to identify mastitis.

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