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Articles by Esmaeil Ebrahimie
Total Records ( 3 ) for Esmaeil Ebrahimie
  Arghavan Alisoltani , Behrouz Shiran , Narjes Rahpeyma Sarvestani , Hossein Fallahi , Naser Aliye Feto and Esmaeil Ebrahimie
  Background and Objective: Microsatellites or simple sequence repeat (SSR) markers play an important role in plant breeding projects. While a large number of SSRs in plants has been recently identified, only a few SSRs have been randomly validated in the lab. Therefore, to cope with the large numbers of SSRs, it is suggested that a targeted selection scheme may be more efficient in identifying functional biomarkers than the random selection of SSRs. The aim of this study was to develop a new method for identifying functional SSR markers in plant breeding. Methodology: For this study, in silico analyses of available wheat RNA-seq data under heat SSR stress were conducted and unique SSR patterns were obtained. In addition, alterations of SSRs under other stress conditions were confirmed through RNA-seq data of rice subjected to salt, drought and cold. The Audic and Claverie, R of Stekel and Falciani Fisher and General Chi-squared tests were all applied for comparisons of data. Results: The results of the study revealed that GC/GC and GCC/GGC repeats were significantly more common under stress conditions compared with controls for both wheat and rice samples. Interestingly, genes containing these motifs have been found to participate in abiotic stress responses and to include various heat-shock proteins (HSPs) and DREB/CBF (DRE-binding protein/C-repeat binding factor) proteins. Conclusion: The overall findings of this study suggest the possibility of using genes with altered SSRs as functional markers. The applied workflow and the results presented here are expected to help establish a new paradigm for future studies involving genetic diversity, breeding, molecular biology and association studies of plants grown under adverse environmental conditions.
  Esmaeil Ebrahimie , Abdolhadi Hosseinzadeh , Mohammad Reza Nagavi , Mohammad Reza Ghannadha and Manijeh Mohammadie-Dehcheshmeh
  Rapid and genotype-independent protocols for two direct in vitro morphogenesis pathways including direct shoot organogenesis from embryo and direct shoot proliferation from node have been developed in cumin (Cuminum cyminum L.). Direct regenerations occurring without passing callus phase are important since fewer somaclonal variation and genotype-dependency are likely to arise from these methods in comparison with regenerations trough callus. After embryo culture, shoots with single-cellular origin were regenerated from the meristematic zone of embryo without any intermediate callus phase. In contrast, proliferated shoots with multi-cellular origin were directly regenerated from the axillary buds (meristems) of node explants. Effects of different concentrations of 6-Benzylaminopurine (BAP), α-Naphthaleneacetic Acid (NAA) and Indole-3-kcetic Acid (IAA) on B5 medium of embryo and node cultures as well as subculture were studied in detail. In direct organogenesis pathway from embryo explant, 0.1 mg L-1 NAA + 1 mg L-1 IAA resulted the highest shoot regeneration response (89.5 shoots per regenerated explant), whereas 0.1 mg L-1 BAP + 1 mg L-1 NAA was the most effective combination in direct shoot proliferation from node explant (42 shoots per regenerated explant). BAP (cytokinin) revealed the inhibitory effect on induction of direct shoot organogenesis pathway from embryo explant, while low concentration of BAP (0.1 mg L-1) had positive effect on direct shoot proliferation pathway from node explant. Subculturing was not necessary for shoot multiplication and elongation in embryo culture, whereas multiplication and elongation of shoots in node culture were associated to subculture on growth regulator-free medium. In other part of study, the behavior of different cumin genotypes in direct regeneration pathways was studied. Both direct organogenesis and direct proliferation pathways were applicable to different cumin genotypes and regenerated plants were phenotypically normal. This study supports the feasibility of combined direct regenerations protocols from embryo and node of cumin in germplasm conservation by in vitro cloning and genetic improvement programs.
  Manijeh Mohammadi-Dehcheshmeh , Ahmad Khalighi , Roohangiz Naderi , Esmaeil Ebrahimie and Manoochehr Sardari
  Fritillaria imperialis is an endangered bulbous plant and therefore in vitro micropropagation of this plant will have a great importance for germplasm conservation and commercial production. Petal explants, for the first time, were cultured on media containing various concentrations of plant growth regulators. In addition, the effects of cold pretreatment and light on induction and regeneration of somatic embryogenesis trough callus were studied in detail. Cold pretreatment had inhibitory effects on somatic embryogenesis pathway. Among the different combinations of 6-Bnzylaminopurine (BAP), α-naphthaleneacetic acid (NAA) and indole-3-aceticacid (IAA) tested, B5 medium supplemented with 0.1 mg L-1 BAP + 0.6 mg L-1 NAA + 0.4 mg L-1 IAA was the best treatment for bulblet production (6 bulblets per somatic embryogenesis callus). This research presents petal as a reliable material for micropropagation and germplasm conservation of Fritillaria imperialis.
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