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Articles by Endang Sutriswati Rahayu
Total Records ( 3 ) for Endang Sutriswati Rahayu
  Siti Helmyati , Mohammad Juffrie , Endang Sutriswati Rahayu and B.J. Istiti Kandarina
  The role of gut microbiota in human health is highly understandable. Recent research highlights the influence of geographical terms in gut microbiota composition. Unfortunately, baseline data of microbiota composition in children are limited. We compared the number of fecal microbiota of children ages 9-12 years, living in Kulon Progo (KP), Yogyakarta, with same age children in West Lombok (WL) and West Nusa Tenggara. The study was an observational study with comparative design. The subjects were elementary school students, 29 subjects in KP and 27 subjects in WL. Fecal microbiota was analyzed by pour plate culture technique on MRS agar for lactic acid bacteria (LAB) and TBX agar for E. coli. The difference of total bacteria was tested using independent sample t-test. Mean of total fecal LAB of children in KP was 7.58±0.61 CFU/g, whereas in WL was 7.19±0.83 CFU/g. There was no significant difference between the number of fecal LAB of children in KP and in WL (p = 0.255). Mean of total fecal E. coli of children in KP was 7.06±0.75 CFU/g. whereas in WL was 7.23±1.12 CFU/g. There was no significant difference between the number of fecal E. coli of children in KP and in WL (p = 0.078). Further research is needed to determine the factors that can affect the composition of the gut microbiota.
  Sri Supadmi , Agnes Murdiati and Endang Sutriswati Rahayu
  Background and Objectives: Mocaf reportedly contains a high content of amylose, which functions as an iodine carrier. Iodine is attached in a complex bond to its linear chain or to the branched chain of amylopectin. Evaluation of the absorption of iodine attached to amylose and amylopectin was performed to measure the amount of absorbed iodine in the small intestine. The absorption rate must be studied since iodine deficiency leads to health problems in all ages, especially affecting children’s growth and development. This study was designed to evaluate in vitro iodine absorption of potassium iodate (KIO3)-fortified mocaf in the small intestine. Materials and Methods: An in vitro evaluation of iodine absorption was conducted using the everted gut sac method. Data were statistically analyzed using one-way analysis of variance (ANOVA) followed by Duncan’s Multiple Range Test (DMRT). Results: The highest absorption occurred at 75 and 90 min, while the highest concentration was 40 ppm. The absorption rates at various concentrations were 56.23% (0 ppm), 65.53% (10 ppm), 69.29% (20 ppm), 71.91% (30 ppm) and 84.65% (40 ppm). The KIO3 concentration significantly affected iodine absorption (p≤0.05). Conclusion: The highest absorption rate was 89.10%. A higher KIO3 concentration increased iodine absorption and a longer absorption period tended to enhance the absorption rate.
  Isti Handayani , Tyas Utami , Chusnul Hidayat and Endang Sutriswati Rahayu
  Background: Lactobacillus plantarum Dad-13 is a probiotic lactic acid bacteria strain that produces uricase for reducing uric acid. Objective: The aim of study was to evaluate the stability of intracellular uricase produced by L. plantarum Dad-13 in the gastrointestinal system and to enhance the production of this enzyme. Materials and Methods: Lactobacillus plantarum Dad-13 was grown on Peptone Glucose Yeast extract (PGY) medium supplemented with uric acid as inducer. The stability of intracellular uricase was evaluated in the stomach and small intestine models. For enhancement of uricase production, L. plantarum was grown on variation of incubation time, uric acid concentration and temperature, while glucose residue, intracellular uric acid were used as the criteria of evaluation. Data were statistically analyzed by one way-ANOVA followed by DMRT. Results: The intracellular uricase of L. plantarum Dad-13 remained active in the gastrointestinal system. Uricase is an inducible enzyme produced when glucose residue limited in the medium. This bacteria uptake uric acid from the medium during growth and uricase activity was obtained at the optimum concentration of uric acid in the cell. A maximum uricase activity was reached at 0.15% uric acid concentration and 37°C for 22 h of incubation. Conclusion: Production of intracellular uricase produced by L. plantarum Dad-13 which have activity in gastrointestinal tract could be enhanced by optimum fermentation and make it applicable for hyperuricemia treatment.
 
 
 
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