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Articles by Endah Sulistyawati
Total Records ( 2 ) for Endah Sulistyawati
  Alfi Rumidatul , Endah Sulistyawati and I Nyoman Pugeg Aryantha
  Background and Objectives: Sengon Falcataria moluccana (F. moluccana) has the ability to synthesize protease inhibitors, such as trypsin inhibitors, that can inhibit the activity of an enzyme produced by Uromycladium tepperianum (U. tepperianum), a fungus that causes gall rust disease. The purpose of this research is to study the activity of the trypsin inhibitor, the total protein content and the protein profile in healthy and gall rust infected sengon. Materials and Methods: The samples used in this research were taken from sengon twigs consisting of healthy and gall rust infected bark and wood. In this research, three age classes of sengon were used: 1-2 years, 3-4 years and 5-6 years. The method used in this research was field sampling, trypsin inhibitor activity analysis, protein determination and protein profile analysis by SDS-PAGE (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Results: The results showed that, in general the activity of trypsin inhibitors in the gall rust infected sengon twigs was higher than in the healthy sengon twigs. The highest protein content was found in gall rust infected bark twigs and the lowest protein content was in healthy wood twigs. The results of the SDS-PAGE analysis of healthy and gall rust infected bark showed that there was a protein with a molecular weight of 21 kDa, which was identified as a trypsin inhibitor. Conclusion: Falcataria moluccana infected with U. tepperianum fungus showed an increase in the activity of trypsin inhibitors and protein content.
  Aos , Endah Sulistyawati and Tati Suryati Syamsudin
  Background: Root distribution of annual crops is critical for agroecosystem management on sloping dry land. The objectives of this study are to examine the root interactions at intercropping system using combination of annual crops of five species (Rice, maize, peanut, red bean and cassava) and to understand their effects on root distribution and soil physical conditions (Bulk density and soil porosity). Materials and Methods: Seven combinations of annual crops ("Rice+maize", "Peanut+maize", "Red bean+maize", "Peanut+cassava", "Red bean+cassava", "Rice+cassava" and "Red bean") were observed by measuring root biomass, root length, bulk density and soil porosity. Results: Combination of "Red bean+maize" resulted in larger biomass of thin root at 0-5 cm soil layer, longer Root Length Density (RLD) of fine root at 10-15 cm soil layer, longer RLD of fine and thin root at 15-20 cm soil layer, decrease in bulk density and increase in soil porosity. The result of PCA indicates that vertical root distribution is restricted if biomass of fine root is concentrated at 0-5 and 5-10 cm soil layers, if Root Area Ratio (RAR) of fine root is concentrated at 0-5 cm soil layer and if RAR of thin root is concentrated at 15-20 cm soil layer. Horizontal root distribution is restricted if biomass of fine root is concentrated at 10-15 cm soil layer and if biomass of thin root is concentrated at 15-20 cm soil layer. The parameters related to bulk density are RLD of thin root in soil layer 10-15 cm, RLD of fine root in soil layer 5-10 cm and RAR of thin root in soil layers 5-10 and 15-20 cm. Conclusion: The combination of "Red bean+maize" has higher ability of root penetration and it improves the physical conditions of the soil.
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