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Articles by Elsayed Elsayed Hafez
Total Records ( 2 ) for Elsayed Elsayed Hafez
  Tarek H. Taha , Saad A. Alamri , Hesham M. Mahdy and Elsayed Elsayed Hafez
  Bioavailability and environmental stress are problems affecting Poly Aromatic Hydrocarbons (PAH) biodegradation. This study aims to overcome the effect of PAH bioavailability via biosurfactant production and the effect of environmental stresses via using different immobilization matrices. Three different PAH bacterial degraders (A2, P5 and N7) were immobilized in different immobilization matrices. The immobilization matrices used in this investigation were: ca-alginate, agar-agar and agarose. RAPD-PCR, plasmid profile and 16S rRNA sequencing methods were used to identify and group the bacterial isolates. The production of biosurfactant was detected using the methylene blue analysis procedure. The results indicated that P5 and N7 isolates preferred the alginate matrix compared to the agar and agarose matrices, where biosurfactant production was 136 and 165.5 mg L-1 for both isolates, respectively. However, the A2 isolate produced a higher biosurfactant concentration (132.4 mg L-1) when grown on agarose. The preferred matrices for the three isolates were different in the presence of hydrocarbons. The A2 and P5 isolates preferred agar as the best matrix for biosurfactant production. On the other hand, the free cells of the N7 isolate produced the highest concentration of biosurfactant compared to immobilized cells. The overall results of this study showed that the type of preferred immobilization matrix depends on the used carbon source; where, in general, calcium alginate was preferred at the presence of glucose while agar was preferred at the presence of hydrocarbons.
  Gamal Mohamed Hamad , Tarek Hosny Taha , Ali Mohammed Alshehri and Elsayed Elsayed Hafez
  Background and Objective: Production of glutathione through mutated yeast strains and the possible usage as antibacterial agent against some food borne bacteria. Also, the antioxidant activity of the glutathione considered as another value when added as food supplement and food preservative. Moreover development of a commercial medium mostly consists of secondary products for the high yeast propagation and high amount of the produced glutathione. The aim of this study was producing a mutated strains of Saccharomyces cerevisiae (S. cerevisiae) and Candida utilis (C. utilis); have high capability to produce high amount of glutathione. Materials and Methods: In this regard, two yeast strains; Saccharomyces cerevisiae and Candida utilis were examined for glutathione production. For increasing the production of the two yeast strains; chemical mutation using Ethyl methanesulfonate (EMS) was approached and the mutated strains were selected on medium contains the glutathione analogue. Comparison of means was performed with Duncan's multiple range test using Costat software. Results: Only two mutated strains showed high ability to produce glutathione with 49 folds more than wild types (MG40/S.C/4 and MG20/C.U/5). Moreover, the antioxidant capacity for the two mutated strains was 9 folds increased compared to wild types. The fermentation process was performed to analyze different parameters and it was observed that the medium should contains molasses as carbon source, yeast extract as a nitrogen source, KH2PO4 of mineral and cysteine for amino acid. Conclusion: Glutathione could be used as antioxidant and antibacterial against wide range of human pathogens bacteria in addition used as food additive, supplement and food preservation to control human pathogenic bacteria.
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