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Articles by Elfahmi
Total Records ( 3 ) for Elfahmi
  Tina Rostinawati , Catur Riani , Elfahmi , Yeyet Cahyati Sumirtapura and Debbie Sofie Retnoningrum
  In this recent study, Tyr93 of Bacillus sp. A2-5a (BA2-5a) cyclodextrin glucanotransferase (CGTase) was replaced with Phe residue by site-directed mutagenesis to study its role on the product specificity and kinetic properties. Molecular docking approach was also applied to acquire a detailed analysis of enzyme-substrate interaction. The Y93F was overproduced in Escherichia coli BL21 (DE3) and purified by Ni-NTA resin. The purified Y93F CGTase was 76.39 kDa based on 10% SDS-PAGE analysis and showed both β-cyclization and starch hydrolysis activities in a zymography assay. There was no major structural conformational change in the Y93F since, its optimum and stability of temperature and pH were the same as the wild type. The substitution did not alter the cooperativity property of the enzyme. Our work provided the new evidence that Y93F substitution caused no α-cyclodextrin (CD) formation in BA2-5a CGTase. In addition, the Y93F produced more β-CD and decreased its kcat and kcat/Km. In conclusion, Y93F substitution of BA2-5a CGTase alters the enzyme kinetic property and product specificity.
  E.Y. Sukandar , Nurdewi and Elfahmi
  Guazuma ulmifolia Lamk. (mutamba) and Curcuma xanthorrhiza Roxb. (java turmeric) has been used traditionally as slimming agent and for treating various diseases including hypercholesterolemia. In this study, the effect of aqueous extract of mutamba leaves in combination with ethanol extract of java turmeric has been determined on lipid blood level of Wistar rats compared to each extract alone in hyperlipidemic induced rats. Thirty male rats weighing 180-200 g were divided into six groups receiving mutamba extract, java turmeric extract, simvastatin as a reference of antihyperlipidemic drug, combination of mutamba and java turmeric at two different doses and one control group. The extract was given daily for 14 days at the same time as the administration of propylthiouracil and food high in cholesterol content to induce hyperlipidemia. The serum levels of total cholesterol, triglyceride, HDL and LDL were measured after 3, 7 and 14 days of treatment. The result were analyzed statistically using ANOVA. The results indicated that combination of aqueous extract of mutamba leaves at a dose of 25 mg kg-1 b.wt. and ethanol extract of Java turmeric rhizome at a dose of 12.5 mg kg-1 b.wt. decreased total cholesterol and LDL level significantly compared to control group (p = 0.004) after 7 days of treatments. Aqueous extract of mutamba leaves at a dose of 50 mg kg-1 b.wt. did not show any significant effect while ethanol extract of java turmeric rhizome at a dose of 25 mg kg-1 b.wt. decreased only LDL level significantly.
  Sukrasno , Kartika , Irda Fidrianny , Elfahmi and Khairul Anam
  Curcuma xanthorrhiza is one the most important of Indonesian crude drug. Its pharmacological activities are usually associated with curcuminoid and the essential oil content. This study was aimed at studying the influence of the crude drug preparation on the content and the composition of volatile oil Curcuma rhizome. The bulky rhizomes were stored at room temperatures and their volatile oil contents measured. The composition of the oil was analyzed by GC-MS. The rhizomes were sliced, dried, comminuted and their oil content measured. Storage of bulky rhizome at room temperature continuously decreased the yield of volatile oil from bulky fresh rhizome. Storage did not change the number and the identity of the oil component: However, it altered their composition. Germacron, xanthorrhizol and α-curcumene increased while dipi-α-cedren decreased during storage. Upon slicing, drying under the sun, drying in an air oven, grinding and storage of the dried Curcuma rhizome, most of the volatile oil was still retained in the crude drug. Storage of bulky Curcuma rhizome continuously decreased its volatile oil content and the lost of oil reach 57% after 12 weeks. It is suggested that to assure the highest level of volatile oil content in the preparation of Curcuma crude drug, the rhizome should be immediately sliced and dried after harvest.
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