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Articles by Ekowati Handharyani
Total Records ( 1 ) for Ekowati Handharyani
  Hidayah Dwiyanti , Hadi Riyadi , Rimbawan Rimbawan , Evy Damayanthi , Ahmad Sulaeman and Ekowati Handharyani
  A sixteen weeks experiment was conducted to evaluate the effect of feeding CPO and RPO enriched palm sugar on liver retinol and immunoglobulin G (IgG) concentration in vitamin A depletion rats. Thirty-four-weeks old-male Sprague Dawley rats were received the standard diet (AIN-93M) for two weeks as adaptation period, after that 24 rats followed by vitamin A-free diet, for 10 weeks as a depletion period and of six remaining rats were given the standard diet as a control group. The depleted rats were then divided into 3 groups and received daily for 4 weeks as repletion period either a red palm oil (RPO) or crude palm oil (CPO) enriched palm sugar, or retinyl palmitate (RE) which each treatment contain approximately 40 μg beta carotene. Rats were blood-sampled at week 2 and week 4 and liver retinol and immunoglobulin G (IgG) concentration were determined. The body weight was taken every 5 days. Increasing on liver retinol concentration in rat administered retinyl-palmitate was higher than in rat administered CPO or RPO enriched palm sugar (p = 0.0136) which the increased in RE, CPO and RPO groups of 115.4, 86.4 and 61.7%, respectively. However, administering of enriched palm sugar both of CPO and RPO for 2-4 weeks can improve vitamin A status to above cut off point which increase from 0.063 μmol/g liver up to 0.107-0.117 μmol/g liver in CPO group and 0.079-0.102 μmol/g liver in RPO group. On the other hand, serum immunoglobulin G (IgG) level of animal administered palm sugar containing CPO or RPO were higher than in rat administered retinyl palmitate (p = 0.0073), which is indicated by increasing levels of serum IgG in both groups of CPO and RPO, that is equal to 225 and 216.01 percent, respectively compared to RE group of 59.98 percent. From this research we conclude that coconut palm sugar enriched with CPO or RPO were potentially as food-based intervention to overcome vitamin A deficiency.
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