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Articles by Edokpolor Osazee Ohanmu
Total Records ( 2 ) for Edokpolor Osazee Ohanmu
  Edokpolor Osazee Ohanmu and Beckley Ikhajiagbe
  Background and Objective: Environmental stress such as heavy metal is a major factor affecting reduction in agricultural productivity. Heavy metals at toxic levels hamper normal plant functioning and act as an impediment of metabolic processes by the production of Reactive Oxygen Species (ROS). Plant has evolved various enzymatic and non-enzymatic mechanism to cope against the ROS produced. This study investigated the biochemical response of S. stenocarpa to cadmium stress. Materials and Methods: S. stenocarpa (Fabaceae) seeds were sown in the control (0 ESV) and the different concentrations (2.5, 5 ESV) of cadmium chloride (CdCl2). The soil samples were collected from 10 different points at a depth of 0-30 cm using a soil auger, pooled together, air-dried and grind to pass through a 2 mm sieve before subjecting to physico-chemical analysis. The five accessions consisted of 3 blocks, 15 treatments per block, two plant per polythene bag arranged in a RBD. Enzymes such as superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), proline as well as non-enzymes such as tocopherol, ascorbate were determined. Results: Increased cadmium concentration resulted in increased translocation of the metal in the plant leaves and decreased chlorophyll a/b content of TSs-accessions. Increase in cadmium concentration results to variability in enzymatic and non-enzymatic response of the studied accessions. Take for instance overall MDA, SOD and proline activities were significantly increased in the Cd-5ESV as compared to the control. However, the accessions varied in their response to cadmium. For instance, the SOD of TSs-95 was reduced with increased cadmium concentration. Similarly, the ascorbate and tocopherol content decreased in TSs-92 and TSs-95, respectively with increased Cd concentration. Individual plants showed different antioxidant responses either between the plants or among the accessions. The MDA activity significantly increased in leaves with metal increase while SOD activity was heighten in TSs-91 whereas in TSs-95 there were no significant difference in SOD activity between control and Cd-exposed plant. Conclusion: The increase in antioxidant activities of the metal exposed plants compared to their control counterpart suggested possibility of free radicals instigated by metal presence. This further gave trendies to plants’ resistance capacities with SOD been the first line of defense.
  Edokpolor Osazee Ohanmu and Beckley Ikhajiagbe
  Background and Objective: With the increased soil pollution caused by heavy metals, identifying accessions that are high nitrogen assimilators has a potential in improving soil fertility and increase crop yield. Therefore, the current study aimed at determining the variation in nitrogen assimilation and biomass accumulation of cowpea accessions to cadmium pollution. Materials and Methods: A field experiment was conducted to evaluate the plant nitrogen assimilation and dry weight matter of cowpea accessions in response to cadmium pollution in Benin city located in southern Nigeria. Five cowpea accessions (TVu-91, TVu-92, TVu-93, TVu-95 and TVu-96) were sown in the control (0ESV) and two treatments of cadmium chloride (Cd-2.5ESV and Cd-5ESV), laid out in a randomized block design (RBD) and replicated thrice. The leaves and roots were assessed at 6 and 18 weeks after sowing and (WAS) for total nitrogen, nitrogen assimilated as nitrate-N or ammonia-N form and their percentage. The plants’ biomass accumulation was also determined 20 WAS. Results: From the result, 6 WAS cadmium pollution significantly reduced foliar TN% irrespective of cowpea accession except in TVu-95. Cowpea accessions distributed nitrogen mainly as nitrate-N with the highest values observed in TVu-91 while TVu-96 recorded the highest N% assimilated as nitrate irrespectively of treatment. In addition to this, cadmium pollution reduced the overall foliar yield and plant dry weight of TVu-accessions with increased metal concentration. TVu-92 and TVu-96 in the Cd-2.5ESV and Cd-5ESV were more closely related in their mode of response to cadmium incidence compared to the other accessions. Conclusion: Nitrogen was significantly assimilated more as nitrate-N than ammonium-N and readily distributed to the leaves.
 
 
 
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