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Articles by Ebrahim Azizi
Total Records ( 8 ) for Ebrahim Azizi
  Sepideh Arbabi Bidgoli , Bagher Minaee , Mohammad H. Ghahremani , Mansoor Djamali Zavarei , Shamileh Fouladdel and Ebrahim Azizi
  The present study was carried out to determine the TP status in colorectal cancer and to correlate molecular alterations with sex and other clinicopathological findings. Out of 129 surgically resected colorectal cancer patients, 51 tumor samples (23 males, 28 females) were randomly analyzed by immunohistochemical techniques using primary antibody for TP and LSAB2 detection kit. Despite the lack of significant correlation between patients’ sex and most other clinicopathological parameters, the mean tumor size in males (5.7174±1.8453 cm) was significantly (p=0.016) more than females (4.4643±1.8453 cm) in this study. Out of 51 postoperative colorectal tumor samples, 24 (47.1%) showed positive TP expression. Unlike other clinicopathological parameters, TP immunostaining was significantly correlated with tumor size (p=0.007), lymphatic invasion (p= 0.036) and sex of patients (p=0.002). The prevalence of TP positive immunostaining was significantly higher in males than females (66.6% versus 33.3%, respectively). Due to the importance of high TP expression in predicting the tumor responses to fluoropyrimidines, the results of the present study possibly show the role of sex hormones in TP expression and angiogenesis. This finding might be important in being considered as a valuable prognostic or predictive marker in clinical settings.
  Shamileh Fouladdel , Zahra Motahari and Ebrahim Azizi
  In the present study, the protein expression of cyclin D1 was determined in human breast cancer T47D cells and its TAM-resistant subline (T47D/TAMR-6 cells). The resistant subline was established in vitro following stepwise increase in TAM concentration in the culture medium of parent cells. Immunocytochemical method was used to determine the expression of cyclin D1 in both parent and resistant cells. The slow growing T47D/TAMR-6 cells showed lower level of expression of cyclin D1 protein compare to parent T47D cells. Expression of cyclin D1 protein was significantly decreased in both cell types following exposure to TAM (1 μM). Therefore, it can be suggested that TAM can regulate the expression of cyclin D1 protein that affects the cell proliferation. In conclusion, determination of protein expression of cyclin D1 in tumor samples of patients would be helpful in tumor prognosis and prediction of outcome of chemo-hormonal therapy of breast cancer.
  Shamileh Fouladdel , Zahra Motahari , Sepideh Arbabi and Ebrahim Azizi
  In the present study, the protein expression levels of Thymidylate Synthase (TS) and Thymidine Phosphorylase (TP) were determined in human breast cancer T47D cells and its derived TAM resistant subline (T47D/TAMR-6 cells). The resistant subline was established in vitro following stepwise increase in TAM concentration in the culture medium of parent cells. Immunocytochemical method was used to determine the expression of TS and TP enzymes in both parent and resistant cells. The slow growing T47D/TAMR-6 cells showed lower level of expression of both proteins compare to parent T47D cells. Expression of TS was also observed to be more than TP in both studied cell types. In conclusion, in patients with tumor immunophenotype similar to these two cell lines, the efficacy of 5-FU chemotherapy and its congeners will be expected to be different. This in turn would affect the disease prognosis and therapeutic predictions. Therefore, the outcome for 5-FU-containing chemotherapy regimens when applied after tumor progression on hormonal therapy is better to be weighed against the status of TS and TP protein levels.
  Sara Rasoul-Amini , Ali Khalaj , Abbas Shafiee , Mohsen Daneshtalab , Armin Madadkar-Sobhani , Shamileh Fouladdel and Ebrahim Azizi
  New cytotoxic quinoline derivatives were designed, synthesized and evaluated in vitro as anti-tumor agents in comparison to available drugs including Adriamycin (ADR), Vincristin (VCR), Etoposide (VP16) and Tamoxifen (TAM). Human breast cancer T47D cells were cultured in RPMI 1640 complete culture medium and exposed for 48 h to different concentrations of newly synthesized quinoline derivatives [SRA-HX-(1-3) and SRA-BQ] and also to ADR, VCR, VP16 and TAM. A dose-dependent decrease in cell proliferation was observed following exposure to almost all synthesized quinolines. The highest cytotoxicity was seen at 1x10-4M concentration of SRA-HX-3 that was near to growth inhibitory effect of ADR (1x10-6M) and significantly (p<0.002) greater than VCR, VP16 and TAM (each at 1x10-6M). The other 3 compounds (1x10-4M) had similar activity to VCR that was less than ADR and significantly (p<0.002) greater than VP16 and TAM. Therefore, new cytotoxic quinolines are potentially good candidates for further investigation as anti-tumor compounds.
  Sepideh Arbabi Bidgoli , Bagher Minaee , Mansoor Djamali Zavarehi , Shamileh Fouladdel and Ebrahim Azizi
  Comparative study of markers of drug resistance in cancer tissues may be extremely helpful in selection of effective chemotherapeutic regimen. P-glycoprotein (P-gp) and Topoisomerase II α (Topo II α) are two fundamental proteins in multi-drug resistance phenomenon (MDR). This study determined the expression and significance of P-gp and Topo II α proteins in advanced gastric carcinomas and correlated molecular alterations with clinicopathological findings. Tissue samples of 35 patients with advanced type gastric adenocarcinoma were analyzed. Immunohistochemical techniques were applied using primary antibodies for P-gp or Topo II α and LSAB2 detection kit (Dako-Denmark). Positive immunostaining for P-gp and Topo II α were observed in 42.9% and 17.2% of tumor samples, respectively. Negative expressions of P-gp and TopoII were associated with sex (p = 0.035 and 0.0001) and histological grade (p = 0.021 and 0.0001), respectively. Unlike P-gp, an inverse relationship between Topo II α expression and size of tumors (p = 0.012) and lymphatic invasion (p = 0.013) were observed. Considering the key role of positive P-gp or negative Topo II α expression in MDR, it could be concluded that groups of patients with P-gp over expression (42.9%) or lack of Topo II α expression (82.8%) would less likely benefit from available chemotherapeutic regimens. Therefore, we highly recommend determination of tumor status for expression of tumor markers such as P-gp and Topo II α, before deciding about effective chemotherapeutic regimen for patients with gastric cancer.
  Elham Haji-Sayyari , Saeed Noukar , Mehran Mohseni , Mohammad Reza Fazeli , Hossein Jamalifar and Ebrahim Azizi
  The potential mutagenicity of two Nickel-Chromium based alloys (Verabond II and Minalux) was determined using the Ames Salmonella/microsome test. According to ISO 6871-1 protocol, the alloys were mounted in an artificial saliva medium and incubated for 7 days at 37°C. Then aliquots were used to assess mutagenic effects of the released materials using Salmonella typhimurium strain TA 100 and the standard plate incorporation assay in the presence or absence of S9 fraction from rat liver. No mutagenic effects were detected for either alloys at 25, 50, 100 μL of test solutions. The S9 bioactivation also showed no mutagenic metabolite activities for these alloys. Therefore, it seems that both alloys have no mutagenic activity with or without bioactivation under the test conditions.
  Shamileh Fouladdel , Bagher Larijani and Ebrahim Azizi
  Due to controversial reports on the relationship between diabetes and cancer, we decided to look at the effects of Tamoxifen (TAM) in the presence and absence of high glucose concentrations on proliferation of human breast cancer T47D cells and its Tamoxifen resistant subline (TAMR-6 cells). Both cell types were separately grown in 24-well culture plates for up to 4 weeks in the RPMI1640 culture medium containing TAM, different concentrations of glucose, combination of TAM and glucose and control (RPMI). Trypan blue dye exclusion method was used to evaluate the in vitro cytotoxicity of test compounds. Data indicated a time and dose dependent antiproliferative effect of glucose on T47D cells at studied concentrations. Results also showed that the cytotoxicity of glucose was significantly greater on parent T47D than TAMR-6 cells (p<0.01). A significant synergistic effect was also observed between TAM and glucose on both cell types (p<0.01). In conclusion, present results indicate that high glucose concentrations similar to diabetic conditions exert antiproliferative effects in breast cancer T47D cells.
  Nasrin Samadi , Mojgan Alvandi , Mohammad Reza Fazeli , Ebrahim Azizi , Hadi Mehrgan and Mansour Naseri
  Polymerase Chain Reaction (PCR) was performed to verify the utility of this technique in detecting low levels of microbial contamination in quality control of pharmaceutical products. Universal and specific primers were applied to identify Staphylococcus aureus at both genus and species levels, as one of the objectionable microorganisms in pharmaceuticals especially topical products. Samples were deliberately inoculated with a defined number of bacterial cells and subsequently exposed to mild lysis. The crude lysate was subjected to PCR amplification. Agarose gel electrophoresis revealed amplified fragments as predicted, with no interference from other bacterial strains included in the study. The sensitivity of the assay was about 102 cfu mL-1 which improved to 1 cfu mL-1 after an overnight preenrichment. Combination of this simple lysis and rapid PCR protocol provided a highly sensitive and practical approach in detecting trace amounts of Staphylococcus aureus contamination in pharmaceutical preparations. In contrast to conventional culturing methods that require a mean of 5-6 days for identification of microorganisms, the entire mentioned PCR assay lasted about 1-2 h.
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