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Articles by E.J. Morales
Total Records ( 2 ) for E.J. Morales
  G. Tellez , G.M. Nava , J.L. Vicente , M. De Franceschi , E.J. Morales , O. Prado , J.C. Terraes and B.M. Hargis
  A trial was conducted to evaluate the influence of dietary Aspergillus Meal (AM) prebiotic on intestinal development, morphology in turkey poults. Day-old Nicholas poults (n = 100) were randomly assigned to two groups. Poults in each group were divided and placed in floor pens with 50 poults each. One group served as a control and received a ration containing no added AM prebiotic. Poults in the treatment group were provided a ration supplemented with 0.2% AM prebiotic. Each dietary regimen and water were provided ad libitum to 30 days of age. Poults were humanely killed by CO2 inhalation and specimens of duodenum and distal ileum were collected at 10, 20 and 30 days. Histology sections were cut (5 μm) and stained with haematoxylin and eosin and combinations of either of periodic acid-Schiff with alcian blue, or high iron diamine with alcian blue stains to evaluate 20 intact villi on each section. The morphometric variables analyzed included villi height, villi surface area and crypt depth. In addition, goblet cells, classified as neutral, acidic or sulpho mucin cells, respectively, were quantitative for each treatment group. At all times of evaluation, AM prebiotic significantly increased the number of acid mucin cells in the duodenum, neutral mucin cells in the ileum and sulpho mucin cells in the duodenum and ileum. Villi height and villi surface area of both duodenum and ileum were significantly increased at days 10 and 30 compared to control. The present study suggest that AM prebiotic has an impact on the mucosal architecture and goblet cells proliferation in the duodenum and ileum of neonate poults.
  L.M. Vargas-Rodriguez , J.G. Herrera , E.J. Morales , J.L. Arcos-Garcia , R. Lopez-Pozos and G. Ruelas
  The objective of this experiment was to evaluate the effect of citric acid (CA), phytase and calcium in diets of laying hens on the productive performances and digestibility and excretion of phosphorus (P), calcium and nitrogen (N). An experiment was designed using 24 week old laying hens, which were fed a diet with a base of sorghum and soybean meal containing 2,700 kcal ME/kg, 15% crude protein, 3.25% calcium and 0.25% available phosphorus. The following were also added to the diets of hens depending on the treatment groups: 0.0, 0.6 and 1.2% citric acid, 0.0 and 300 units of phytase/kg of diet (FTU) and 3.00 and 3.25% calcium. The phytase was added as an ingredient into to diets, contributed to 0.1% of phosphorus and 0.3% of calcium levels. The experiment involved 12 treatments in a 3 × 2 × 2 factorial design. The treatment 0.6% citric acid, 300 FTU and 3% calcium increased the digestibility of phosphorus, resulting in its decreased excretion (p<0.05). Citric acid decreased the excretion of calcium and N linearly (p<0.01), increased (p<0.05) their digestibility and had effect on the response of the phytase (p<0.05). The treatment of 1.2% citric acid, 300 FTU and 3.25% calcium increased (p<0.05) the digestibility of N quantitativaly. It can be concluded that citric acid reduces the excretion levels and increases the digestibility of P, N and calcium and has effect on the response of the phytase to affect the excretions of P and N.
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