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by
E.F.P. Henie |
Total Records (
2 ) for
E.F.P. Henie |
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Z.A. Zakaria
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C.A. Fatimah
,
A.M. Mat Jais
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H. Zaiton
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E.F.P. Henie
,
M.R. Sulaiman
,
M.N. Somchit
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M. Thenamutha
and
D. Kasthuri
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The present study was carried out to investigate on the possible antibacterial activity of aqueous (AEMC), methanol (MEMC) and chloroform (CEMC) extracts of Muntingia calabura using the in vitro disc diffusion methods. The sterilized blank discs (6 mm diameter) was impregnated with 20 μL of the respective extract (in the concentration of 10,000, 40,000, 70,000 and 100,000 ppm) and tested against Corneybacterium diphtheria, Staphylococcus aureus (ATCC 25923), Bacillus cereus, Proteus vulgaris, Staphylococcus epidermidis, Kosuria rhizophila, Shigella flexneri, Escherichia coli (O 157), Aeromonas hydrophila and Salmonella typhi. At all concentrations tested, the AEMC was effective against S. aureus and K. rhizophila while the MEMC was effective against S. flexneri, B. cereus, S. aureus, P. vulgaris, A. hydrophila, K. rhizophila. This activity was not observed with the CEMC. At the concentration of 40000 ppm and above, the AEMC exhibited significant antibacterial activity against C. dihptheriae, P. vulgaris, S. epidermidis and A. hydrophila; the MEMC was effective against C. diphtheriae and L. monocytogenes; and the CEMC was effective against S. aureus. Finally, we concluded that M. calabura possesses a potential antibacterial property that is comparable to the standard antibiotics used. The results also suggest the presence of more potent polar antibacterial compound. |
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Z.A. Zakaria
,
H. Zaiton
,
E.F.P. Henie
,
A.M. Mat Jais
and
E.N.H. Engku Zainuddin
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The present study was carried out to determine the in vitro antibacterial activity of aqueous and chloroform extracts of the leaves and fruits of Averrhoa bilimbi L. using the standard disc diffusion test. The air-dried leaves and fruits of A. bilimbi were separately soaked in distilled water or chloroform (1:10; wv-1) for 24 h and the resultant supernatants were filtered. The aqueous extract was freeze-dried while the chloroform extract was evaporated to dryness under reduce pressure to obtained the crude dried extracts, which were prepared in the concentration of 25, 50 and 100 mg mL 1 prior to the antibacterial assay. The results obtained demonstrated the potential of A. bilimbi extracts against some of the Gram-positive and-negative bacteria. The 100 mg mL 1 aqueous extract of A. bilimbi’s leaves and fruits showed positive antibacterial activity against the Gram-positive S. aureus, S. epidermis B. cereus, C. diphteriae with the latter also gave positive effect against K. rhizophila. Both extracts also exhibited positive antibacterial activity against the Gram-negative S. typhi, C. fuendii and A. hydrophila. On the other hand, the 100 mg mL 1 chloroform extracts of A. bilimbi’s leaves and fruits demonstrated antibacterial activity against the Gram-positive S. aureus, S. epidermis, B. cereus, K. rhizophila and C. diphteriae and Gram-negative S. typhi, C. fuendii, A. hydrophila and P. vulgaris. In conclusion, the A. bilimbi leaves and fruits possess potential antibacterial activity that requires thorough study. |
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