|
|
| |
| Articles
by
E.B.G. Jones |
Total Records (
2 ) for
E.B.G. Jones |
|
 |
| |
|
| |
Umaiyal Munusamy
,
Vikineswary Sabaratnam
,
Sekaran Muniandy
,
Noorlidah Abdullah
,
Ashok Pandey
and
E.B.G. Jones
|
| |
Four strains of Pycnoporus sanguineus isolated
from Thailand (KUM 60953), Shah Alam (KUM 60954), Endau Rompin (KUM 60955),
Cherating (KUM 60956) and Gombak (KUM 60957) were grown on PDA plates.
Laccase of P. sanguineus was produced using oil palm frond parenchyma
tissue (OPFPt) in solid substrate fermentation (SSF). Strain KUM 60954
produced significant (p = 0) levels of laccase at 2.53 U mL-1
(76 U g-1) followed by KUM 60957 at 1.05 U mL-1
(32 U g-1), KUM 60956 at 0.55 U mL-1 (17 U g-1)
and KUM 60955 at 0.46 U mL-1 (14 U g-1). Meanwhile,
KUM 60953 (reference strain) from Thailand produced 2.44 U mL-1
of laccase (73 U g-1 of OPFPt). Biodegradation of a mixture
of 10 ppm each of phenanthrene, anthracene and pyrene by 30 U mL-1
of laccase in sodium citrate buffer pH 5 was studied. The reaction mixture
was incubated at 40°C and was shaken at 80 rpm for 24 h. Laccase of
KUM 60954 degrades 90% of phenanthrene, 37% of anthracene and 96% of pyrene.
Meanwhile, laccase of KUM 60953 degrades 89% of phenanthrene, 43% of anthracene
and 95% pyrene. Pyrene was rapidly biodegraded followed by phenanthrene
and anthracene. However, a similar pattern of degradation was observed
for both KUM 60953 and KUM 60954. Degraded PAH sample was further tested
for toxicity using Artemia. The untreated PAH caused more than
50% of Artemia death while for the treated PAH no death was observed
indicating that the toxicity level was reduced and possibly no any new
toxic compound was produced during the degradation. |
|
|
| |
|
| |
Umaiyal Munusamy
,
Vikineswary Sabaratnam
,
Sekaran Muniandy
,
Noorlidah Abdullah
,
Ashok Pandey
and
E.B.G. Jones
|
| |
Four strains of Pycnoporus sanguineus isolated
from Shah Alam (KUM 60954), Endau Rompin (KUM 60955), Cherating (KUM 60956),
Gombak (KUM 60957) and the standard strain from Thailand (KUM 60953) were
evaluated for their ability to grow on Polycyclic Aromatic Hydrocarbon
(PAH)- and acetonitrile-incorporated Potato Dextrose Agar (PDA). The mycelial
growth was then compared to the growth of P. sanguineus on PAH
and acetonitrile free PDA. All strains tested showed a high tolerance
to PAH up to 25 ppm and 2.5% of acetonitrile. Laccase derived from the
best strains KUM 60954 and KUM 60953 which exhibited higher tolerance
to both PAH and acetonitrile was further characterised. The activity was
maximum for both strains at pH 5.0 and 40 °C. Laccase from both strains
was stable over a wide range of pH (3-5) and at temperatures below 60 °C.
Laccase was stored as a concentrate or as a lyophilised powder to minimise
the degradation of enzyme activity during storage. The laccase activity
in the extract remained a high activity up to four months at optimum pH
5.0 and at both 0 and 4 °C. Further laccase was also stable up to 24
h at 40 °C when mixed with 1% of acetonitrile. |
|
|
|
|
| |
|
