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Articles by E.A. Azab
Total Records ( 2 ) for E.A. Azab
  H.A. Murad , Sahar H. Mohamed , Asmaa G. Abu-El- Khair , E.A. Azab and Maha A. Khalil
  For improving the quality of the popular low fat Kariesh cheese it was manufactured from skimmed buffalo's milk supplemented with different concentrations ranged from 0.01-0.05% of xanthan gum as a fat replacer. The resultant cheese was microbiologically analyzed for Lactobacillus dlebreuckii ssp., L. bulgaricus, Streptococcus thermophilus on MRS agar and M17 agar, respectively at 37°C for 24 h. The total bacterial count was also determined at 37°C for 24 h. Chemical analysis was achieved for determination of fat, total nitrogen, ash and total solids as recommended by AOAC methods. The texture of the product was evaluated using the double compression test with addition of xanthan gum no alteration of microbial population was detected. The increase of cheese yield upon addition of 0.4 and 0.5% was not significant compared with the control. Textural characteristics including, hardness, cohesiveness, springiness, gumminess and chewiness were increased for both the product and the control cheese up to 15 days storage period after that the hardness and cohesiveness were significantly decreased with increasing xanthan gum concentration. Kariesh cheese made with using 0.04 and 0.05% xanthan gum exhibited high acceptability compared with the control after 7 days of storage period. The addition of xanthan gum as a fat replacer upon manufacturing of Kariesh cheese enriched its flavor and improved its quality.
  Kh.M. Sharshar and E.A. Azab
  The present study was aimed at isolation and characterization of the pathogenic bacterium from diseased freshwater prawn. The effect of the bacterial pathogen on hepatopancreas, gills and exoskeleton was also investigated. Diseased freshwater prawn, Macrobrachium rosenbergii were collected from commercial hatchery in Behera Governorate, Egypt. The diseased prawn showed dark brown focal lesions and necrosis of appendage tips. The causative bacterial pathogen was isolated from haemolymph and hepatopancreas of the diseased prawn. Based on the morphological, biochemical and physiological characteristics, in addition EPI 20E test, the isolated pathogen was characterized as Vibrio vulnificus. Histopathology, hepatopancreas showed haemocytic infiltration in the interstitial sinuses, thickening and ruptures of the basal lamina and necrosis of its tubules. Similarly, the accumulation of haemocytes in the haemocoelic space, swelling, fusion of lamellae and abnormal gill tips. Also, the cuticular layers of the exoskeleton of diseased prawn had a rough or wrinkled surface and were disrupted and separated from the epidermis. The pathogen, V. vulnificus showed different degrees of sensitivity to different antimicrobial agents. It was highly sensitive to each of the antibiotics rifadin, virbamycin, oflaxcin, garamycin, flummox and trimethoprim/sulfamethoxzole) and resistant to nalidixic acid, unasyn, velosef, claforan, negram and amoxicillin. The minimal inhibitory concentration of trimethoprin/sulfamethoxzole for the studied pathogen, V. vulnificus was 0.31/5.93 μg.
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