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Articles by E. Purwati
Total Records ( 3 ) for E. Purwati
  Husmaini , M.H. Abbas , E. Purwati , A. Yuniza and A.R. Alimon
  The main objective of this research is to investigate the potential of LABs isolated from processing byproduct of the VCO in in vitro conditions for use as probiotics in poultry. Forty eight LABs were isolated and four of them have been selected for further study i.e. Lh1, Lh2, Lh3 and Lh4. A series of tests carried out by studying the ability of bacteria to survive at 37 and 42oC, tolerance of LAB at pH 2, 0, 5, 7 and 7, 0 and tolerance to gastric juice as well as sensitivity to several antibiotics commonly was given to poultry. The survival of LABs was evaluated after 15, 30, 60, 90 and 120 and 300 min of incubation. The sensitivity test to antibiotics was performed by Muller Hinton′s agar. All the bacteria showed tolerance and ability to grow at pH 5 and 7, but only Lh4 enabled to tolerate at pH 2. All of LAB can grow at gastric juice stimulated. Lh4 was not sensitive to all antibiotics (clear zones: 0.33 mm) but the other LABs were sensitive (clear zones: 5-12 mm). The conclusion of this research is the ability of LABs to grow in in vitro conditions varies. The Lh4 has demonstrated its ability to grow and the best survival with the OD (λ = 580) is 1.99 after 300 min of incubation at pH 2 and has shown the most resistant to all antibiotics tested with a wide clear zone 0.33 mm, hence potentially be used for probiotic in poultry.
  Sabrina , M.H. Abbas , E. Purwati , Y. Heryandi and Robby
  This study was conducted to evaluate the effects of altitude and dietary protein level on performance of local ducks. Two hundred and forty of female Pitalah ducks 14 weeks old were used in this study. Ducks reared on high altitude (H) and low altitude (L). The research was carried out by using Split-plot arrangement in a Randomized Block Design with the H and L as main-plot and the dietary protein level (PL: T-1 = 14, T-2 = 16, T-3 = 18% protein) as sub-plot. Treatments were done when duck was 16 weeks old. Observations were made after the production of duck was 10 %. The environment and rectal temperature were recorded daily. Variables such as: feed intake, protein intake, egg production (Duck day and Egg mass) and Feed Conversion Ratio (FCR) were observed weekly. The altitude were highly significantly (P<0.01) influenced on feed intake, protein intake, egg production (Duck day and Egg mass) and Feed Conversion Ratio (FCR). PL were not significant (P>0.05) affect to feed intake but significantly (P<0.05) increased duck day production and highly significant (P<0.01) increased egg mass and FCR. There was no interaction (P>0.05) between altitude and dietary of protein level. The results of this study showed that the performance of the duck raised on the H was better than L. The increasing of protein level up to 18% have been given the best performance of Pitalah duck.
  Husmaini , Sabrina , F. Arlina , E. Purwati , S.N. Aritonang and H. Abbas
  Objective: A trial was conducted to evaluate the effects of age at administration of Lactococcus plantarum isolates from virgin coconut oil processing waste on the number of Lactic Acid Bacteria (LAB) in the intestine and the growth performance of broilers. Methodology: The research used 160 day old cobb broilers divided into 4 treatment groups: T0 (without LP), T1 (Lactococcus plantarum administered at 1 week of age), T2 (2 weeks of age) and T3 (3 weeks of age). The basal diet consisted of corn, rice brain, fish meal, soy bean meal, bone meal, vegetable fat and premix (21.1% crude protein and 3038 kcal kg–1 energy metabolism). Chickens were given Lactococcus plantarum only one time and were slaughtered every week until 5 weeks old. Variables included the number of LAB, E. coli and Salmonella in the intestine, thickness and length of the intestine, carcass weight, fat and cholesterol content of carcass, body weight, feed intake and feed efficiency. The data were evaluated using a one-way ANOVA. Results: The results showed that Lactococcus plantarum administration affected the balance of microflora in the gut and the length of the intestine. Lactococcus plantarum treatment significantly increased the number of LAB in the intestine (p<0.01) up to 2 weeks after administration, conversely, the number of E. coli and Salmonella decreased. When given at 2 and 3 weeks, the effect of Lactococcus plantarum increased intestinal length and broiler growth performance was highly significant (p<0.01). Probiotic treatment did not affect carcass percentage but affected both the abdominal fat and cholesterol of broiler meat. Conclusion: Optimal body weight, feed conversion ratio (1.78) and cholesterol content were observed when Lactococcus plantarum was given at 2 weeks of age.
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