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Articles by E. Nwachukwu
Total Records ( 3 ) for E. Nwachukwu
  P.O. Nwiyi , E. Nwachukwu and I.G. Erumaka
  The appropriate use of disinfectants in poultry farm is an important and major component of a successful bio-security program. The objective of the study was to determine the most effective of all the disinfectant in reducing microbial load, the effect of time on disinfectant and the effect of organic waste on disinfectant. Salmonella organism place in a broth and the disinfectant reconstituted with distill water in 1:10 dilution was introduced. The opacity tube was used as a standard for clearing. Salmonella gallinarum was used to represent pathogenic gram negative bacteria commonly found in poultry farms. The first study is to determine the most effective of all the disinfectant in reducing the microbial load. The disinfectant used are phenol, gluteraldehyde and quaternary ammonium compound. The second study deals with the effect of time (0, 2, 4, 8 and 12 weeks) on the efficacy of disinfectants diluted to working concentrations. The third study determined the effect of organic waste on the concentration of freshly prepared disinfectants and 12 weeks old disinfectantsin the presence of organic waste. The result of the first study showed that the most effective of all the disinfectants under consideration is quaternary ammonium compound. While the result of the second study show that time affected the efficacy of disinfectant as freshly reconstituted disinfectant gave better efficacy than 12 weeks old disinfectant, against Salmonella organism. The result of the third study show that organic waste reduces the efficacy of disinfectant (p≤0.05) remarkably.
  E. Nwachukwu , O.M. Onovo and C.F. Ezeama
  The bacterial quality of zobo drinks locally produced and the effect of lime juice on the bacteria associated with the drinks were investigated. Zobo drinks were obtained from local market and analysed bacteriologically according to standard methods. The total viable counts was 2.79 log10 cfu mL-1 and total coliforms was 2.62 log10 cfu mL-1. Bacteria isolated from zobo drink samples included Staphylococcus aureus, Bacillus subtilis, Lactobacillus sp. Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes, Klebsiella sp. The isolates in decreasing order of occurrence were Staphylococcus aureus (45%), Escherichia coli (40%), Lactobacillus sp. (37%), Enterobacter aerogenes (32%), Pseudomonas aeruginosa (30%), Klebsiella sp. (26%) and Bacillus subtilis (23%). The total coliforms and total viable counts generally decreased in values following treatment of zobo drink samples with lime juice. The study revealed that lime juice can be used to prolong the shelf-life of zobo drinks.
  E. Nwachukwu and C.M. Emeruem
  Sachet water samples produced locally were examined for quality and antibiotic resistant bacteria. The total viable counts and types of bacteria in the water samples were determined using standard bacteriological techniques. The number of organisms obtained from the water samples ranged from 1.2x101 to 3.0x102 cfu/100 mL. Bacterial isolates resistant to ampicillin were 80.4% while 77.2% were resistant to tetracycline and 23.7% were resistant to ofloxacillin. The presence of these antibiotic resistant organisms in drinking water is of public health importance and may be responsible for prolonged treatment of water borne diseases. Therefore proper diagnosis and appropriate administration of drugs will help in controlling the increase of antibiotic resistant bacteria. However, proper treatment of water before consumption is recommended.
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