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Articles by E. Dirandeh
Total Records ( 2 ) for E. Dirandeh
  H. Kohram , G. Mohammadi and E. Dirandeh
  The objective of the present study was to evaluate the ovarian response of buffaloes to GnRH injections given at different moments of the estrous cycle. The estrous cycles of 15 buffaloes were synchronized with 2 iM injections of prostaglandin F2 given 11 day apart. The buffalos were randomly assigned to 1 of 3 groups. Buffaloes in the control group received no treatment, whereas GnRH6 buffaloes received a GnRH injection between day 5 and 7 and GnRH16 buffaloes received a GnRH injection between day 15 and 17 of the estrous cycle (estrus = day 0). Daily, from estrous cycle day 0 to the next estrous cycle day 23, cows had their ovaries scanned by ultrasound. All follicles were classified in to 4-6 mm and 7 mm follicles. Data were analyzed using the GLM procedure of SAS. In the 3 treatments, the number of 4-6 mm follicles had a decline trend from day 1 and reached their least amount on day 4 and 5 (p<0.05). Thereafter, the number of this class of follicles in the control, GnRH6 and GnRH16 treatments increased until day 8, 10 and 6, respectively (p<0.05). In response to an injection of GnRH, the number of small follicles in GnRH6 and GnRH16 treatments increased on day 9 and 18 respectively. Again, the number of small follicles increased in the control and GnRH6 groups from day 15 and 19, respectively, to day 21 of the estrous cycle. The number of 7 mm follicles had an increase (p<0.05) until day 3 in the control and 5 in the GnRH6 and GnRH16 groups. In the GnRH6 treatment of buffaloes, GnRH injection on day 6 increased (p<0.05) the number of large follicles on day 10. The number of 7 mm follicles in the two groups (control and GnRH6) increased between day 17 and 19 of the estrous cycle. An increase the number of small and medium sized follicles 2 day after GnRH injection showed that an injection of GnRH at the beginning or later days of the estrous cycle could promote the emergence of a new follicular wave in buffaloes.
  A. Zare Shahneh , Z. Mohammadi , H. Fazeli , M. Moradi Shahre Babak and E. Dirandeh
  Ovarian functional disorders are reported as an important cause of infertility in dairy cattle. This experiment was carried out to determine the effects of GnRH injection at the time of insemination on ovulation rate, conception rate and plasma progesterone concentrations on day 5 post AI. Egithy cows were randomly assigned into 2 experimental groups: Treatment group (n = 40) which received 15 ug of GnRH and control group (n = 40) without any hormonal treatment. Cows were palpated per rectum to ascertain the occurrence of ovulation 24 h post AI. Blood samples were drawn on the day of insemination and on the day 5 post AI. Plasma concentrations of P4 were measured by RIA and analyzed by means of the GLM procedure of the SAS. Differences between the two experimental groups were analyzed by one way ANOVA. Ovulation rate and conception rate were analyzed by means of Binary Models of the SPSS. Results of this study indicated that the injection of GnRH increased the ovulation rate insignificantly (65% vs 47.5%, p>0.05) and increased conception rate significantly (55% vs 25%, p<0.03). GnRH administration caused an insignificant increase in the concentration of plasma progesterone on day 5 post AI (6.03 vs 5.27 ng mL-1, p<0.02). In general results of this study indicate that GnRH injection at the time of artificial insemination improves ovulation and pregnancy rates.
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