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Articles by Dyah Maharani
Total Records ( 3 ) for Dyah Maharani
  Dyah Maharani , I Gede Suparta Budisatria and Panjono
  Objective: The melanocortin-1-receptor (MC1R) gene is known as an important candidate gene for the coat color trait. The present study identified the polymorphisms and tested whether a functional polymorphism in exon 1 of MC1R gene was related to different head and neck color in Ettawa grade goat based on their allele distribution. Methodology: Total 30 Ettawa grade goats were divided in three group, CP: Brown head and neck color with white body color, RP: Brown or black head and neck color with various body color and HP: Black head and neck color with white body color were used in this study. The blood samples from all groups were collected for DNA isolation. Some samples were used to identify the polymorphisms by direct sequencing. Three Single Nucleotide Polymorphisms (SNPs) of MC1R gene were identified: SNP 676A>G, SNP 748T>G and SNP 801C>G. The SNP 676A<G was performed for genotyping by using EarI restriction enzyme. Results: The A and G allele frequencies were similar 50% in both CP and RP group. However, the A allele frequency (55%) was slightly higher than G allele (45%) in HP group. The A and G allele distribution in all the group population was nearly the same (X2 = 0.016, df = 1, p = 0.898). All of the goats in CP and RP groups had heterozygote (AG) genotype. In HP group only had one goat with homozygote animal (GG genotype). Interestingly, none AA genotype found in this study. Conclusion: In conclusion, the allele distributions were equal in all groups and almost all the goats with different head and neck color have similar heterozygote genotype.
  Ayu Rahayu , Dattadewi Purwantini , Dyah Maharani and Tety Hartatik
  The objective of this study was to investigate the variations in Melanocortin 1 receptor (MC1R) gene and the association with plumage colours in Magelang duck populations using single nucleotide polymorphism (SNP). Forty three genomic DNA samples of Magelang ducks consisted of 3 plumage colour groups of brown, black and white were investigated. Primer design from Anas platyrhynchos complete genome in GenBank Accession Number HQ699486. Primer F:5’-GCTCTTCATGCTGCTGATGG-’3, R:5’-GATGAAGACGGTGCTGGAGA-’3. A 256-bp fragment was amplified by polymorphism chain reaction then sequenced. Two SNPs identified in this study were c.376A>G and c.409G>A. Chi-square (X2) value showed that 0.75 in Magelang ducks had GG genotype; 0.81 for GA genotype; 0.34 for AA genotype (c.376G>A) and 0 for GG genotype; 1.37 for GA genotype; 5.82 for AA genotype (c.409A>G). These results suggested that the MC1R genotype distribution in Magelang duck population with brown, black and white plumage was balanced or not deviated from Hardy Weinberg Equilibrium. Conclusively, two SNPs were identified; c.376A>G SNP that changed the amino acid from isoleucine to valine and valin/isoleucine and c.409G>A SNP that changed alanine to threonine and threonine/alanine.
  Dyah Maharani , Wihandoyo , Galuh Adi Insani , Laurentia Adinda and Dwi Nur Happy Hariyono
  Objective: The study was carried out to characterize the qualitative morphological traits of Indonesian native chickens. Materials and Methods: Forty adult male chickens made up of 4 chicken groups based on different comb types (single, pea, rose and walnut) were visually appraised for the qualitative traits. Data on qualitative traits were analyzed using descriptive statistics and compared as percentages. Results: In single group, the majority of chickens have dark yellow beak (40%), red-brown crown (50%) and neck feather (50%), black for the feather of chest (50%), abdomen (50%), back (60%), primary (60%) and secondary wings (90%) and tail (40%). In Pea group, the majority of chickens possessed light yellow beak (50%), red-brown crown (40%) and neck feather (40%), white-brown chest feather (40%), black for the feather of abdomen (40%) and back (30%) and black-green tail (40%). In Rose group, most of chickens possessed light-yellow beak (40%), red crown (40%), yellow neck feather (50%), red for the feather of chest (40%) and abdomen (40%), black back feather (60%) and black-white tail (60%). In Walnut group, the majority of chickens have pale yellow beak (50%), red-brown crown (80%) and neck feather (60%), black for the feather of chest (40%), abdomen (40%) and back (30%) and black-white tail (40%). Dark eye, black primary and secondary wings, black thigh feather, light yellow membrane shank and yellow shank were predominant in all chicken groups. Conclusion: Indonesian native chickens possess a considerable phenotypic diversity that could be important for the genetic improvement through systematic breeding strategies.
 
 
 
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