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Articles by Dyah Iswantini
Total Records ( 4 ) for Dyah Iswantini
  Dyah Iswantini , Nadinah , Latifah Kosim Darusman and Trivadila
  Apium graveolens, one of the traditional medicinal plants, has a potential as anti-gout. We have reported that flavonoid of A. graveolens could inhibit activity of xanthine oxidase enzyme up to 85.44%. The aim of the research was to investigate the type of inhibition kinetic of A. graveolens ethanol crude extract and its fraction inhibition kinetic, also to determine the active compound. The result of the research showed that the yield of A. graveolens ethanol crude extract was 10.40 % (LC50 1968.19 mg L-1) with the inhibition activity was 6.04% until 74.01% (100-2000 mg L-1). Inhibition kinetic of 1500 mg L-1 crude extract caused increased KM (0.10 mM) and unchanged Vmax. Based on these data, the type of inhibition was competitive. Purification of crude extract resulted 7 fractions and the highest activity was achieved by fraction 6 (inhibition activity was 85.08%). The purification of crude extract caused the increasing of inhibition activity effect. Inhibition kinetic of fraction 6 (150 mg L-1) caused increase KM (0.30 mM) and unchanged of Vmax. Based on that, the type of inhibition was competitive. Purification of fraction 6 resulted 6 fraction and the highest activity was achieved by fraction 5 (inhibition activity was 88.41%). Based on analysis of LCMS and NMR, the active compound of A. graveolens extract (fraction 5) were potential to inhibit the activity of xanthine oxidase, the active compound was 5, 7-dihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one and 2, 3-dihydro-6-hidroxy-5-benzofuran carboxylic acid.
  Saputera , Djumali Mangunwidjaja , Sapta Raharja , Leonardus B.S. Kardono and Dyah Iswantini
  Identification and taxonomy analysis conducted at Herbarium Bogoriense at Research Centre for Biology, Indonesian Institute of Sciences Bogor. The name of the plant was C. tiglium L. The result of analysis on C. tiglium, ethanol extract as laxative material using the intestinal transit method showed treatment group that received dosage 0.06 mL/30 g b.wt. (72.5%) was significantly different compared to negative control (48.4%) or positive control (50.6%) which showed the weak effect as laxative at the dosage of 0.75 mL/30 g b.wt. It showed that ethanol extract of C. tiglium seed at dosage 0.06 mL/30 g is effective as laxative. The test result of the treatment using dosage 0.06, 0.04, 0.026 and 0.07 mL/28 g of body weight showed the mice population response 100, 60, 40 and 40% consecutively. The Thompson and Weil analysis result showed the ED50 was at 0.027 mL or equal to 639,5 g kg-1 b.wt. The LD50 was at 0.0707 equals with 1674,5 mg kg-1 b.wt. Safety limit is the range of dosage that cause the lethal effect and the dosage that gives the intended effect. The safety limit is represented by the comparison of LD50/ED50. Calculation result that the extract safety limit was LD50/ED50 = 0.0707/0.027 = 2.7.
  Dyah Iswantini , Novik Nurhidayat , Trivadila and Okik Widiyatmoko
  Determination of uric acid concentration in human urine and blood is needed to diagnose several diseases, especially the occurrence of kidney disease in gout patients. Therefore, it is needed to develop a simple and inexpensive method for uric acid detection. The purpose of the research was to observe the use of Indonesian microbe that was immobilized on natural zeolite as a source of uricase for uric acid biosensor. Selection of mediators and determination of optimum condition measurement, the stability and kinetic properties of L. plantarum uricase were performed using carbon paste electrode. Cyclic voltammetry was employed to investigate the catalytic behavior of the biosensor. The result indicated that the best mediator for measurement of L. plantarum uricase activity was Qo (2,3-dimethoxy-5-methyl-1,4 benzoquinone). Optimum conditions for immobilization of L. plantarum uricase on zeolite were obtained at pH 7.6, with temperature of 28°C, using uric acid concentration of 0.015 mM and zeolite mass at 135 mg KM and VMax of L. plantarum uricase obtained from Lineweaver-burk equation for the immobilization uricase on zeolite were 8.6728x10-4 mM and 6.3052 mM, respectively. KM value of L. plantarum uricase directly immobilized onto the electrode surface was smaller than KM value of L. plantarum uricase immobilized on zeolite. The smaller KM value shows the higher affinity toward the substrate. The Electrode when kept at 10°C was stable until 6 days, however the immobilized electrode on zeolite was stable until 18 days. Therefore, Indonesian L. plantarum could be used as a uric acid biosensor.
  Yuliana Tandi Rubak , Lilis Nuraida , Dyah Iswantini and Endang Prangdimurti
  Background and Objective: Lactic acid bacteria with its proteolytic system hydrolyzes proteins to produce angiotensin-I-converting enzyme inhibitor during fermentation. A total of 108 indigenous lactic acid bacteria isolated from fermented food were screened based on proteolytic activity for the potential formation of angiotensin-I-converting enzyme inhibitory activity in fermented soy milk. Materials and Methods: Indigenous lactic acid bacteria (from tempe, kefir and breast milk) were screened based on proteolytic activity. Semi-qualitative screening of proteolytic activity of lactic acid bacteria was performed on skim milk agar. Thirty lactic acid bacteria isolates were further selected based on the formation of peptides in 11% reconstituted skim milk. A total of 10 lactic acid bacteria isolates with high proteolytic activity were selected as starter cultures for soy milk fermentation at 37°C until pH 4.6 was reached. Evaluation of fermented soy milk was performed by enumeration of lactic acid bacteria population, analyses of titratable acidity, soluble protein content, peptide content and determination of angiotensin-I-converting enzyme inhibitory activity. Results: Of 108 lactic acid bacteria isolates, 13.8% isolates had strong proteolytic activity and 15 isolates (13.8%) had moderate proteolytic activity based on clear zones formed surround the colony on skim milk agar after 48 h of incubation. The amount of peptide produced by isolates with strong and moderate proteolytic activity and the ability to reduce pH of soy milk varied among isolates. The pH value of 4.6 of fermented soy milk was reached after 24-48 h of incubation. Of 10 selected isolates, Lactobacillus plantarum 1W22408 and Lactobacillus fermentum R6 produced the highest angiotensin-I-converting enzyme inhibitory activity in fermented soy milk. Conclusion: Proteolytic activity and acidification ability of the lactic acid bacteria varied between isolates. Lactic acid bacteria isolates of Lactobacillus plantarum 1W22408 and Lactobacillus fermentum R6 were potential to be used as a starter culture to produce fermented soymilk which has angiotensin-I-converting enzyme inhibitory activity.
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