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Articles by Donglin Zhang
Total Records ( 5 ) for Donglin Zhang
  Yongchang Hao , Xiaoru Zhang , Donglin Zhang , Ying Cheng , Li Du , Wenhua Kuang , Ming Lei , Hanwei Jiao , Chao Qi and Fengyang Wang
  Brucella sp. are pathogenic bacteria that are internalized by host cells upon activation of cell division cycle 42 (Cdc42) to cause Brucellosis. Buffalo are among the many species that are affected by Brucellosis. The objective of this study was to analyze the expression and distribution of Cdc42 small GTPases in buffalo which may help elucidating the molecular events leading to Brucella internalization. Full-length buffalo Cdc42 mRNA were obtained and real-time quantitative polymerase chain reaction was performed to assess the transcription level of Cdc42 in heart, liver, spleen, lung, kidney and intestine. Western blot was used to determine the level of Cdc42 in heart, liver, spleen, lung, kidney and intestine tissue; immunohistochemistry techniques were applied for evaluating the distribution of Cdc42 in tissue. Cdc42 were found to be expressed in heart, liver, spleen, lung, kidney and intestine tissue and the subcellular localization of Cdc42 was predominantly in the cytoplasma. These data provide important anatomical information on the role of Cdc42 in Brucella internalization in the different buffalo tissue.
  Donglin Zhang , Yongchang Hao , Ying Cheng , Li Du , Ming Lei , Hanwei Jiao , Jianing Zhang , Hui Rong , Xiaoxiao Jia and Fengyang Wang
  Brucellosis is an extremely important disease affecting the health of human and a number of animal species (including buffalo) around the world and Brucellae sp. is the causative agent of brucellosis. As an important innate immunity molecule, CD14, involved in protecting a host against invading microorganism including Brucella. To analyze the expression of CD14 and provide new alternatives for elucidating the molecular mechanism of Brucella infection in bufflo, real-time quantitative Reverse Transcription PCR (RT-PCR) and western blot were performed to assess the mRNA level and protein levels of CD14 in heart, liver, spleen, lung, kidney and intestine. The results indicated that CD14 mRNA and protein are ubiquitously present in all buffalo tissues examined with relatively high levels in lung and relatively low levels in intestine. These data provide important anatomical information for studying the role of CD14 played in Brucella infection in the different tissue of buffalo.
  Yongchang Hao , Hanwei Jiao , Li Du , Donglin Zhang , Ying Cheng , Ming Lei , Hui Rong , Jianing Zhang , Xiaoxiao Jia and Fengyang Wang
  Brucellosis is an extremely important disease affecting the health of human and a number of animal species (including buffalo) around the world and Brucellae sp. is the causative agent of brucellosis. The innate immune system is the first line of defense mechanisms that protect hosts from invading Brucella. As an important innate immunity molecule, MyD88 is critical for TLR-mediated activation of the transcription factor NF-κB and the induction of proinflammatory cytokines. To analyze the expression of MyD88 protein and provide new alternatives for elucidating the molecular mechanism of Brucella infection in buffalo, Western blot were performed to assess the expression of MyD88 protein in heart, liver, spleen, lung, kidney and small intestine, immunohistochemistry techniques were applied for evaluating the distribution of MyD88 protein. The results indicated that MyD88 protein are ubiquitously present in all buffalo tissues examined with relatively high levels in lung and relatively low levels in heart and small intestine and the subcellular localization of MyD88 is predominantly cytoplasm and the tissue specificity were observed. These data provide important anatomical information for studying the role of MyD88 played in Brucella infection in the different tissue of buffalo.
  Yanying Zhang , Qiumei Shi , Guisheng Gao , Hai Fang , Cuizhen Chen , Gaili Ren , Huoju Chang and Donglin Zhang
  The detections of the Locus of Enterocyte Effacement Pathogenicity Island (LEE) and High Pathogenicity island (HPI) in 26 E. coli samples from chickens were conducted by PCR. Ler and eaeA genes located in the core of LEE Virulence Island while irp2, fyuA and asn_RNA_intB genes in HPI Pathogenicity Island were all detected. At the same time, O serotype identification of 26 E. coli samples were conducted. The results showed that the positive rate of Ler gene in LEE Virulence Island was 3.85% (1/26) while eaeA was 3.85% (1/26). The rate of fyuA gene in HPI pathogenicity island was 42.3% (11/26) while irp2 was 73% (19/26), irp2+fyuA+ was 42.3% there was no asn_RNA_intB gene. The results of serotype identifications showed that 15 strains related to serotype O4, O91, O78, O107, O38, O111, O88, O53, O24, O9 and O11 except 11 strains. Among 15 strains, O91, O78 and O38 were dominant serotypes whose proportion were 20 (3/15), 20 (3/15) and 26.7% (4/15), respectively. The nucleotide sequence similarities were >95% for 26 strains isolated from chickens.
  Qiumei Shi , Yanying Zhang , QiuYue Wang , Guisheng Gao , Guangping Gao , Hai Fang , Donglin Zhang , Cuizhen Chen and Xiaomei Lv
  To explore the pathogenic mechanism of Salmonella, serotype was identified and enterotoxin gene stn was detected for 47 strains suspected Salmonella in the Eastern part of Hebei Province. According to the bacterial culture characteristic, physicochemical properties and analysis results of K-3401 semi automatic bacteria identification instrument and identification for another time by Chengdu Institute of Biological Products, enterotoxin gene stn was detected by PCR. The 17 strains of Salmonella gallinarum, 14 strains of Salmonella typhimurium, 4 strains of Salmonella pullorum 2 strains of Salmonella paratyphi A, 2 strains of Salmonella group BO, 5 strains of Salmonella bovismorbificans, 3 strains of Salmonella enteritidis were detected from 47 strains of chicken source of Salmonella. The 45 strains of stn gene were amplified successfully in all 47 strains. The carrying rate was 95.7%. Homology of stn gene of test strains of Salmonella was between 94 and 100%. Evolutionary tree display that different serotypes of Salmonella enterotoxin stn gene divided into 5 groups. There was no homology with other bacterium. Chicken source of Salmonella contained 7 kinds of serotypes in the eastern part of Hebei Province. Among them, Salmonella gallinarum was 36.2% (17/47), Salmonella typhimurium which was advantages serotypes was 29.8% (14/47). The carrying rate of enterotoxin gene stn which had relation with bacterial pathogenicity was 95.7% (45/47).
 
 
 
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