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Articles by Dongjun Liu
Total Records ( 3 ) for Dongjun Liu
  Rui Wang , Shan Cong , Ming Cang , Yuzhen Ma , Jianxun Wen and Dongjun Liu
  Bovine ES cells have been isolated but validated bovine ES cell lines have not yet been successfully established. Here, researchers investigated the factors that affect the derivation, proliferation and maintenance of bovine Embryonic Cell (bESC)-like cells including type of feeders (Mouse Embryonic Fibroblasts (MEFs) vs. Bovine Embryonic Fibroblasts (BEFs)), feeder density and inner cell mass isolation procedure. We found that the MEFs work better than BEFs on the process and best at a cell density of 1.25x105 cells/cm2, naturally hatched blastocysts had a short adherence time and high rate of adherence. They were more conducive to bESC-like cell isolation and culture. Culture medium supplemented with IGF promoted proliferation of bESC-like cells but was unfavorable for the maintenance of pluripotency. The findings provide information on the establishment of culture systems for bESC-like cells.
  Jianglong Yuan , Yong Jin , Bing Zhu , Wei Wang , Yu Ren , Haiqing Wu , Fei Hao , Mingtu Nuo , Muzi Jin , Zhigang Wang , Ming Chang , Dongjun Liu and Xudong Guo
  The efficiency of donor cell types in cashmere goat Somatic Cell Nuclear Transfer (SCNT) is undefined. In this study, SCNT was performed using Caprine fetal Fibroblast Cells (CFCs), Caprine Ear Fibroblast Cells (CEFCs) and Transgenic Caprine Fetal Fibroblast Cells (TCFCs) as donor cells to compare the influences of cell type, transgene and sex of nuclear donor fibroblast cells on the SCNT efficiency including fusion and cleavage rates of reconstructed embryos and the birth, postnatal survival and mortality rates of cloned kids. A total of 4,943 reconstructed embryos were obtained. Among them, 3,949 embryos were fused and 3,737 embryos were cultured in vitro leading to a total of 3,094 cleavage embryos. Furthermore, embryo transplantation analysis was conducted on 1,873 cloned embryos with relatively normal morphology. A total of 368 recipient goats were transplanted and 48 goats were born of which 35 goats survived.
  Muzi Jin , Hui Wu , Wenliang Yang , Fei Hao , Dapeng Tai , Jianlong Yuan , Asga , Ming Cang , Xudong Guo and Dongjun Liu
  Research on the embryonic stem cells of large livestock has recently attracted the attention of scholars. However, it is difficult to keep goat embryonic stem cells in an undifferentiated state during cell passaging in culture. In this study, fertilized embryos of Arbas cashmere goats were obtained by superovulation in vivo. The key issues in the culture of Arbas cashmere Goat Embryonic Stem Cells (AgESCs) were explored: the addition of differentiation-inhibiting factors, the selection of medium as well as the passaging, cryopreservation and thawing methods used. This study found that high-quality in vivo fertilized embryos could be cultured in either serum-containing medium or serum-free medium and that AgESCs could be passaged in either medium for 30 generations. The mechanical method was superior to the trypsin digestion method for passaging AgESCs. There was no change before and after cryopreservation and thawing with regard to AgESC morphology, alkaline phosphatase staining, the formation of embryoid bodies, immunofluorescence staining or the PCR detection of pluripotency factors. Finally this study provides the experimental basis for the establishment of goat embryonic stem cell lines.
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