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Articles by Dongfeng Zhao
Total Records ( 2 ) for Dongfeng Zhao
  Heng Yu Hu , Dongfeng Zhao and Qiang Zhang
  Residual oil gasification is a novel approach to extend the life of old oil reservoir using microbes to transform petroleum hydrocarbons into methane which can be exploited or stored in situ. The eutrophic river water could be used to promote the oil degradation and producing methane. When the trace element was added, the methane yield and petroleum hydrocarbons degradation rate was better. The best trace element CoCl2·6H2O concentration was 1.6 mg L‾1, NiCl2·6H2O and Na2MoO4 were 0.34 and 2.4 mg L‾1, respectively. Meanwhile, the methane yield and oil degradation rate were effected by pressure. Microbes grow better in low pressure condition. The eutrophic river water and trace element have the additive effect on petroleum hydrocarbons degradation. So the eutrophic river water turned useful resource.
  Hengyu Hu , Dongfeng Zhao and Qiang Zhang
  Oil degradation bacteria (Bacillus licheniformis) were isolated and identified by using quantitative PCR-DGGE technology. The Bacillus licheniformis quantity was evaluated by its DNA content and its DNA content was measured by amplification of the DNA target gene. So the specific primers and probe of target gene were designed. Then, the difference was analyzed between using single Bacillus licheniformis bacteria and using Bacillus licheniformis compounded with other bacteria to degrade oil. The results showed that after 60 days cultivation, the oil degradation rate was only 5.6% by using single Bacillus licheniformis bacteria and DNA content of the single bacteria is 1.3×106 copies. When the single bacteria were mixed with other bacteria, the oil degradation rate was 30.7% and DNA content of the Bacillus licheniformis bacteria was 3.3×106. But, when the Bacillus licheniformis bacteria were removed, the oil degradation rate of mix bacteria was 21.3% and the DNA content of Bacillus licheniformis bacteria was 0. It showed the primers and the probe had good specificity. The oil degradation rate and DNA content of single Bacillus licheniformis bacteria were both 0 in the sterilization control group. Compared with traditional technology, the quantitative PCR-DGGE technology could accurately analyze the role of single bacteria in compound bacteria community.
 
 
 
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