Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Articles by Dong Zhang
Total Records ( 2 ) for Dong Zhang
  Lu Li , Dong Zhang , Junhai Lian and Huanmin Zhou
  This study aimed to measure the concentration of intracytoplasmic free-Ca2+ in the in vitro matured bovine oocytes at MII stage and in the in vitro fertilized embryos at early different developmental stages 0 h (fertilization), 2 and 15 h (pronucleus stage) and 48 h (2-cell stage) after fertilization using Fura-2/AM as a Ca2+ fluorescent probe and to investigate Ca2+ spatial distribution in the intracytoplasm of bovine eggs at MII stage and 0, 2, 15 and 48 h after fertilization using Ca2+ fluorescent probe Fluo-3/AM. Meanwhile, the fertilized eggs were cultured in Ca2+-free culture medium to examine the effects of extracellular Ca2+ on the early embryo development. The results indicated that the intracellular Ca2+ concentrations of the bovine oocytes at MII stage and 0, 2, 15 and 48 h after fertilization were 84.99 nmol L-1 and 120.05, 229.09, 108.73 and 111.17 nmol L-1, respectively. No pronuleus or cleft eggs were found when cultured in Ca2+-free culture medium. These results suggested that the fertilization induced intracytoplasmic free-Ca2+ increase that pronucleus formation and egg cleavage were Ca2+ dependent that extracellular Ca2+ performed a specific function for the initiation of zygote development and that comparatively, a high level of Ca2+ concentrations was still maintained after pronucleus formation. Moreover, the spatial profile of fluorescence expression illustrated that a low density free-Ca2+ were homogenously distributed in the cytoplasm of oocytes at MII stage, the Ca2+ were concentrated at the cortex zone under the egg plasma membrane at fertilization, the Ca2+ intensity enhanced and spread towards the center of egg from edge at 2 h after fertilization and the Ca2+ were distributed in whole cytoplasm at pronucleus and first cleavage stage. These results demonstrated that extracellular Ca2+ is essential for normal embryonic development and also, confirm that Ca2+ increase is required for bovine oocyte activation at fertilization.
  Yao Wu , Bin Tong , Kaifeng Wu , Seiki Sasaki , Youji Muramatsu , Takeshi Ohta , Hiroyuki Kose , Takahisa Yamada , Dong Zhang and Guoxin Ni
  Growth performance as well as marbling is the main breeding objectives in Japanese Black cattle, the major beef breed in Japan. The Insulin Receptor Substrate 1 (IRS1) gene is reported to be associated with insulin resistance and birth weight in human and has been previously shown to be located within genomic region of a quantitative trait locus for growth-related trait. The previous study detected a Single Nucleotide Polymorphism (SNP), referred to as g.120947716T>C in the promoter region of the IRS1 gene which exhibited significantly different allelic distribution between Japanese Black sires with extremely high predicted breeding value and the sires with extremely low one for Carcass Weight (CWT) and Rib Thickness (RT) in a preliminary study. In this study, researchers analyzed the effect of the IRS1 g.120947716T>CSNP genotypes on the growth-related carcass trait in Japanese Black beef cattle. The SNP was associated with CWT and RT (p = 0.030 and 0.005, respectively) in experiment using 100 Japanese Black sires. This finding suggests possible effect of the g.120947716T>C on the growth-related trait in Japanese Black beef cattle. The IRS1 SNP may be useful for effective marker-assisted selection to increase the beef productivity in Japanese Black beef cattle.
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility