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Articles by Dong Wang
Total Records ( 19 ) for Dong Wang
  Natascha Pogori , Ahmad Cheikhyoussef , Yan Xu and Dong Wang
  The aim of the study was to investigate the extracellular lipase production by the fungus, Rhizopus chinensis CCTCC M201021. The biochemical characteristics of this lipase were also identified. The inducing effect of vegetable oils (soybean oil, sunflower oil, sesame oil, rice bran oil and olive oil), fatty acids (palmitic acid, oleic acid, linoleic acid and stearic acid) and surfactants (Tween 80, sodium dodecyl sulphate, gum arabic and Triton X-100) on lipase production by Rhizopus chinensis CCTCC M201021 was investigated. Soybean oil enhanced lipase production by 102% and was the highest among other oils studied. There was no significant effect on lipase production by surfactants at p<0.05. The unsaturated fatty acids, oleic and linoleic acid significantly enhanced the lipase production by 147%. The optimum temperature for lipase activity was 30°C and the optimum pH was 6.0. The enzyme had stability in the pH range of 4.0 to 10.0 for 1 h. The enzyme retained stability of 60% of the maximum at 70 °C after pre-incubation for 1 h. Zn2+ and Cu2+ ions depressed the lipase activity but Mg2+ and Ca2+ ions were found to stimulate the lipase activity. The enzyme had broad substrate specificity towards both p-nitrophenyl esters and triglyceride substrates since it efficiently hydrolyzed long-chain and short-chain fatty acids but greater preference was observed for the long-chain fatty acids. The lipase was able to hydrolyze both the 1, 3-position and the internal position of triolein.
  Wei Liu , Wenjie Xu , Dong Wang , Zongtian Liu and Xujie Zhang
  As the unit of human knowledge, event is used to describe all kinds of knowledge in the world. Action which is one of the main factors in event describes the changing processes of world states in a specific period of time. As we know, there are different states in different time and the main reason of states changes is caused by action. Therefore, it is necessary to embrace temporal information into the representation about action to describe these changes. In this study, we first proposed a temporal description logic T-ALC, in which the temporal information as a constraint of instances was added to ABox. It provided a kind of decidable approach to deal with the states changes of objects in action. Then, we defined the syntax and semantics of action based on T-ALC. Especially, the semantics of action can be transformed into the changing processes of interpretations and the calculation of new interpretations and ABox were also given. Finally, several inference services of action in different time were studied.
  Dong Wang and Yongjing Lu
  The traditional high-performance deduplication systems are focused on improving the data compression ratio and throughput of a single node, but most of them have ignored the scalability which makes it difficult to be applied to large-scale distributed environments. This paper presents LevelStore, a large scale key-value store for deduplication storage system which optimized three indicators including compression ratio, throughput and scalability. LevelStore introduced a hierarchical feature which improved the disk utilization by introducing container compaction and exploited a sorted in-memory buffer to favor large sequential writes. More importantly, LevelStore can be dynamically and cost-effectively scaled up on demand. Experiments for different datasets are presented and the results show LevelStore can not only greatly improved the performance of a single node but also provided a lot of efficient building blocks for distributed implementation and thus applicable to large scale and distributed systems.
  Zhengrong Xiao , Dong Wang , Liyun Zhang and Jun Liao
  With the rapid development of mobile communication, the interference between different mobile communication systems is more and more complex. The interference between LTE-FDD and LTE-TDD system in coarea and cosite scenarios is studied. The interference scenarios between LTE-FDD and LTE-TDD system is given, the models, simulation results, related analysis and finally the solution to resolve the interference is given which can be used as reference to deploy the LTE-FDD and LTE-TDD systems.
  Ding Pan , Yingmin Zhang and Dong Wang
  Traditional description logic usually used to represent static knowledge. To solve the problem of metadata interoperability in distributed XBRL, the distributed interact model of XBRL was constructed, the distributed characteristics and formal representation requirement of distributed XBRL interact model was analyzed. A distributed temporal description logic DTDLBR and its syntax and semantics was proposed. Finally, the reasoning mapping algorithm was given.
  Dong Wang , Ding Pan and Yingmin Zhang
  Formalization and reasoning is the basic step of quality control mechanism in XBRL, which is one of the most difficult and challenging issue in XBRL development. In this study, the metadata, hierarchical relationship and ordinary-special relationship of XBRL are formalized in description logic DLRBR. An example of metadata and relationships in XBRL financial statement is given. Rules are constructed and equivalence checking and disjoint checking are implemented in Jena. The result is correctly and high-efficiency.
  Yanxin Zhao , Dong Wang , Souravh Bais and Hongxin Wang
  Background and Objective: Alzheimer disease is one of the common types of dementia, which involves progressive neuronal cell's degeneration. The main objective of this study was to evaluate the in vitro anti-choline esterase activity of four phenyl propanoids derivatives (Eugenol, Gallic acid (GA), Sinapic acid and Scopolin) and evaluation of Eugenol in AlCl3 induced dementia in rats. Materials and Methods: The four phenyl propanoids derivatives (Eugenol, Gallic acid (GA), Sinapic acid and Scopolin) were evaluated for in vitro anti-choline esterase activity. For in vivo screening the rats were treated with aluminum chloride at a dose of 175 mg kg–1, for a period of 25 days to produce dementia. Then rats divided in 4 different groups and further evaluated for 10 days of treatment , i.e., Negative control, Standard group and one group received sub maximal dose of rivastigmine along with Eugenol (1.25 mg kg–1 p.o+ 50 mg kg–1 Eugenol, p.o) and other group received Eugenol (50 mg kg–1, p.o) alone. All rats were observed until the 35th day of experimental protocol. The different behavioral and biochemical parameters like GSH, TBARS and Nitrite level were also determined. Neuro-protective activity of eugenol against neuro inflammation produced by AlCl3 was accessed by estimations of two pro inflammatory cytokines (TNF-α, IL-1β) in brain tissues. Results: Rats treated with aluminum chloride (175 mg kg–1, p.o.) produced a significant decline in behavioral and biochemical parameters in rats. The rats treated with Eugenol showed significant reversal of memory deficit Dementia of AD type. Conclusion: The study concluded that Eugenol had a synergistic effect with rivastigmine when used in combination and showed neuro protection against AlCl3 induced dementia of AD type in rats and modulates the neuro inflammation produced by AlCl3.
  Dong Wang , Bo Lin , Huabin Zhu , Haisheng Hao , Chengjiang Wu , Weihua Du and Xueming Zhao
  To study the amplification potential and establish a two-temperature PCR sex identification method, DNA templates prepared from bovine blood, sperm and hair follicles were amplified through two-temperature PCR in this study. Fragments from 207-1413 bp could be amplified successfully while fragments of 1500 bp in length could not be amplified. In further studies we found that an extension step was necessary to amplify longer fragments and as the extension time decreased, the denaturation and annealing times were increased. After optimization we found the best two-temperature PCR to be a reaction using 1.0 mmol L-1 Mg2+, 1.0 unit polymerase and 30 cycles. The amplification results using different equipment and Taq polymerases indicated that the two-temperature PCR can be used for any fragment equal to or <1413 bp with any common Taq polymerase. The kinetic rate of the polymerase was determined to be the factor limiting the amplification length and rate. Additionally, the PCR timing was shortened from 1.5-2.0 h to 30-38 min with the two-temperature PCR. The sex identification of bovine blood, fibroblasts and blastmeres were carried out by turns using this rapid PCR and a rapid sex identification method of cattle embryo was establishment. Both amplification time and cost were saved greatly with this method and we found that the two-temperature PCR was sensitive and fast enough for widespread use in many research areas such as genetics, sex identification, forensics and clinical medicine.
  Dong Wang , Huabin Zhu , Jiaming Guo , Bo Lin , Linbo Zhang , Haisheng Hao , Weihua Du and Xueming Zhao
  Sort reanalysis using flow cytometry is the most common method for determining the purity of X or Y enriched semen. The high cost of this technique (including the required expensive, proprietary machine) limits efforts to improve the technique and to promote develop applications for the sorted semen. In this study, the sperm sex (the presence of the X or Y chromosome) was identified by both rapid PCR and flow cytometry reanalysis. The rapid PCR results showed that the percentages of X and Y sperm were 48 and 52% in unsorted semen, 92 and 8% in X-enriched semen and 17 and 83% in Y-enriched semen, respectively. Reanalysis of the DNA content of the sorted samples revealed that the X and Y sperm frequencies were 92 and 8% in X-enriched semen and 15 and 85% in Y-enriched semen, respectively. The sex ratio of unsorted semen analyzed by PCR did not significantly deviate from the expected ratio of 1:1 and there was no significant difference between the sex ratios of sorted semen samples determined by PCR and flow cytometry reanalysis. These results indicate that we have established an effective, reliable and rapid PCR method to verify the purity of sorted semen. This method should contribute greatly to the improvement of sperm sorting techniques and the development of applications for sorted semen.
  Huabin Zhu , Bo Lin , Jun Chen , Haisheng Hao , Xueming Zhao , Shujing Li , Weihua Du , Tong Qin , Yan Liu and Dong Wang
  A rapid and simple PCR sex identification of embryo is very important for bovine embryo transferring. Many sex identification methods using duplex PCR were established according to Sry gene. But the identification process was affected greatly by more primers interaction. In order to decrease the interference from more primers, researchers explored a simple and rapid PCR Method. The sequences of Amelogenin alleles located at both sex chromosomes were downloaded from GenBank. A pair of sex specific primers was designed to span the 63 bp longer insertion sequence in X chromosome. Bovine samples of blood, fibroblasts and demi-embryos were sexed with these primers. Two-temperature PCR cycling program was used in which the extension step was deleted while the denaturizing and annealing steps were shortened to 1 sec. The results shown ideal identification were obtained and observable amplification were also obtained using even single fibroblast. About 20 bovine embryos were identified by this PCR cycling program and 15 embryos (9 females and 6 males) were transferred. The sexing results were confirmed by the anatomically proven sex after parturition, respectively. The comparison of amplification results between blood samples of bovine and human shows the excellent, specificity to bovine. Thus, a simple, rapid and effective PCR sex identification method was established.
  Yan Liu , Teng Ma , Weihua Du , Haisheng Hao , Dong Wang , Xueming Zhao , Haijing Li , Qiuling Jiang and Huabin Zhu
  Intramuscular Fat (IMF) is an important factor affecting meat quality. The objective of this study was to investigate the expression changes of genes related to IMF formation in muscles of Small Tail Han sheep. These genes include Acetyl CoA Carboxylase (ACC), Fatty Acid Synthase (FAS), Diacylglycerol Acyltransferase 1 (DGAT1), Heart Fatty Acid Binding Protein (H-FABP), CCAAT-Enhancer-Binding Protein α (C/EBPα), Peroxisome Proliferator-Activated Receptor γ (PPARγ), Malic Enzyme ( ME) and Lipoprotein Lipase (LPL). In the longissimus dorsi, IMF content continuously increased with growth and was significantly different (p<0.05) at all 4 months; however, IMF content reached a maximum at 5 months in the gluteus maximus. The gene expression patterns of the 8 genes involved in IMF synthesis mainly followed one of 2 trends: gene expression tended to be lowest at 4 or 5 months and then subsequently increase in the longissimus dorsi whereas gene expression tended to peak at 4 or 5 months and then subsequently decrease in the gluteus maximus. Intramuscular fat content correlated with the expression levels of all genes in the longissimus dorsi and all genes except DGAT1, H-FABP in the gluteus maximus. Remarkably, all of the above correlations between IMF and gene expression levels were positive. In conclusion, the correlation between gene expression levels and IMF content indicate that these genes play an important role in the deposition of IMF in Small Tail Han sheep.
  Tong Qin , Haoyu Wang , Dengpan Bu , Haisheng Hao , Dong Wang , Weihua Du and Huabin Zhu
  The objective of this study was to evaluate the effect of a corn straw or mixed forage diet on endocrine, metabolism and lactation performance in periparturient Holstein cows. Twelve multiparous, periparturient Holstein cows were randomly assigned to two groups and fed a corn straw or mixed forage diet, respectively. The CS diet included 33.8% corn straw and the F:C ratio [Dry Matter (DM)] was 60:40. The MF diet included 3.7% Chinese wildrye, 28.4% alfalfa hay and 26.5% corn silage, the F:C ratio (DM) was 40:60. All cows were fed from weeks 3-8 and Body Weight (BW), Body Condition Score (BCS) and Dry Matter Intake (DMI) were recorded. Milk protein, fat, lactose and Somatic Cell Count (SCC) were determined twice weekly. Metabolite and hormone analyses of blood were made weekly. Results showed that dietary treatments had no detectable effects on BW, BCS, DMI and blood hormones measured. From the 6 weeks of lactation, cows fed MF diet produced more milk (p<0.05) and tended to produce more milk fat (p = 0.07) and protein (p = 0.10) compared with cows fed CS diet. The proportions of milk fat, protein and lactose (%) did not differ between the two dietary treatments (p>0.05). In addition, the milk SCC in the CS group was significantly higher (p = 0.02) than the MF group. Cows fed MF diet experienced more severe Negative Energy Balance (NEB) and had higher concentrations of β-Hydroxy Butyric Acid (BHBA) and Non-Esterified Fatty Acid (NEFA) than cows fed CS diet. Collectively, these results suggest that cows fed MF diet improve lactation performance of periparturient cows but this dietary treatment may have an adverse effect on NEB of periparturient cows.
  Minzheng Jiang , Yumiao Cai , Yu Lu and Dong Wang
  The rod string wear in directional well deflecting section is the key problem to restrict the production efficiency of directional well, the finite element method is adopted according to vibration theory, contact theory and non-linear theory, then the distribution law of contact force between string rod and oil tube will be acquired under the condition of sucker rod actual well conditions. It analyses the relationship between deviation angle and contact force of rod tube and it also comparative analyses the change rule of contact force before and after installing centralizer in the deflecting section. That provides a theoretical basis to slow the wear of directional well sucker rod string and improve its lifetime.
  Erjun Sun , Xiuli Cheng , Dong Wang , Changfu Zhuang , Aiqing Xia and Tongshun Shi
  In this article, a series of porphyrin-nicacid dyads and their Mn and Zn complexes were synthesized and characterized by elemental analysis, UV-Vis, IR, MS, and 1H NMR spectroscopies. Fluorescence studies show that for increasing length of alkoxy chains the fluorescence intensity and quantum yield of the porphyrin ligands is C2 > C3 > C4 > C5 > C6 > C10. Electrochemical results indicate that the length of side chain has little effect on the redox potential.
  Xiong Li , Dong Wang and Zheng Cui
  One new cytisine-type alkaloid, [(3-hydroxy-6-pyridinyl)-methyl]-cytisine (1), was isolated from the stem bark of Maackia amurensis, together with five known alkaloids: cytisine (2), N-formylcytisine (3), N-(3-oxobutyl)cytisine (4), (-)-epibaptifoline (5) and N-methylcytisine (6). The structure of 1 was elucidated based on spectral methods (IR, CD, 1D and 2D NMR, HR-ESI-MS and EI-MS).
  Dong Wang , Xiuli Cheng , Yuhua Shi , Erjun Sun , Xuexin Tang , Changfu Zhuang and Tongshun Shi
  The porphyrin nicotinic acid binary compounds with different substituents in porphine rings (5-(4-nicotinicoxyldecyloxy)phenyl-10,15,20-triphenylporhyrin 2a, 5-(4-nicotinicoxyldecyloxy)phenyl-10,15,20-tri(4-chlorophenyl)porphyrin 2b and 5-(4-nicotinicoxyldecyloxy)phenyl-10,15,20-tri(4-methoxyphenyl)porphyrin 2c) were synthesized. All of them have been characterized, assigned and analyzed by UV–vis, IR, MS and 1H NMR spectra. Their electrochemical and spectroscopic properties were studied by using cyclic voltammetry, fluorescence spectra and Resonance Raman spectra. Different substituents have a little influence on electrochemical behavior and fluorescence spectra. In the Resonance Raman spectra, the substituent has little influence on the skeleton vibration of porphyrin and has much influence on the vibration of phenyl.
  Wei Zhang , Dong Wang , Elzi Volk , Hugo J. Bellen , Peter Robin Hiesinger and Florante A. Quiocho
  The V<SUB>0</SUB> complex forms the proteolipid pore of a vesicular ATPasethat acidifies vesicles. In addition, an independent functionin membrane fusion has been suggested in vacuolar fusion inyeast and synaptic vesicle exocytosis in fly neurons. Evidencefor a direct role in secretion has also recently been presentedin mouse and worm. The molecular mechanisms of how the V<SUB>0</SUB> componentsmight act or are regulated are largely unknown. Here we reportthe identification and characterization of a calmodulin-bindingsite in the large cytosolic N-terminal region of the <I>Drosophila</I>protein V100, the neuron-specific V<SUB>0</SUB> subunit a1. V100 formsa tight complex with calmodulin in a Ca<SUP>2+</SUP>-dependent manner.Mutations in the calmodulin-binding site in <I>Drosophila</I> leadto a loss of calmodulin recruitment to synapses. Neuronal expressionof a calmodulin-binding deficient V100 uncovers an incompleterescue at low levels and cellular toxicity at high levels. Ourresults suggest a vesicular ATPase V<SUB>0</SUB>-dependent function ofcalmodulin at synapses.
  Juan M. Pascual , Dong Wang , Ru Yang , Lei Shi , Hong Yang and Darryl C. De Vivo
  Exon IV of SLC2A1, a multiple facilitator superfamily (MFS) transporter gene, is particularly susceptible to mutations that cause GLUT1 deficiency syndrome, a human encephalopathy that results from decreased glucose flux through the blood-brain barrier. Genotyping of 100 patients revealed that in a third of them who harbor missense mutations in the GLUT1 transporter, transmembrane domain 4 (TM4), encoded by SLC2A1 exon IV, contains mutant residues that have the periodicity of one face of a kinked α-helix. Arg-126, located at the amino terminus of TM4, is the locus for most of the mutations followed by other arginine and glycine residues located elsewhere in the transporter but conserved among MFS proteins. The Arg-126 mutants were constructed and assayed for protein expression, targeting, and transport capacity in Xenopus oocytes. The role of charge at position 126, as well as its accessibility, was investigated in R126H by determining its activity as a function of extracellular pH. The results indicate that intracellular charges at the MFS TM2–3 and TM8–9 signature loops and flanking TMs 3, 5, and 6 are critical for the structure of GLUT1 as are TM glycines and that TM4, located at the catalytic core of MFS proteins, forms a helix that surfaces into the extracellular solution where another proton facilitates transport.
  Manjula Karpurapu , Dong Wang , Nikhlesh K. Singh , Quanyi Li and Gadiparthi N. Rao
  Platelet-derived growth factor BB (PDGF-BB) induced cyclin A expression and CDK2 activity in vascular smooth muscle cells (VSMC). Inhibition of nuclear factors of activated T cell (NFAT) activation by cyclosporin A (CsA) and VIVIT suppressed PDGF-BB-induced cyclin A expression and CDK2 activity, resulting in blockade of VSMC in the G1 phase. In addition, CsA- and VIVIT-mediated inhibition of NFATs and small interfering RNA-targeted down-regulation of cyclin A levels suppressed PDGF-BB-induced VSMC DNA synthesis. PDGF-BB also induced cyclin A mRNA levels in VSMC in an NFAT-dependent manner. Cloning and bioinformatic analysis of rat cyclin A promoter revealed the presence of NFAT-binding elements, and PDGF-BB induced the binding of NFATs to these regulatory sequences in a CsA- and VIVIT-sensitive manner. Chromatin immunoprecipitation analysis showed that NFATc1 binds to the cyclin A promoter in response to PDGF-BB in a VIVIT-sensitive manner. Furthermore, PDGF-BB induced cyclin A promoter-luciferase reporter gene activity in VSMC, and it was inhibited by both CsA and VIVIT. Balloon injury induced cyclin A expression and CDK2 activity in rat carotid arteries, and these responses were also blocked by VIVIT. In addition, VIVIT attenuated balloon injury-induced SMC proliferation, resulting in reduced restenosis. Down-regulation of NFATc1 by its small interfering RNA inhibited PDGF-BB-induced cyclin A expression and DNA synthesis both in rat and human VSMC. Together, these findings demonstrate that the cyclin A-CDK2 complex may be a potential effector of NFATs, specifically NFATc1, in mediating SMC multiplication leading to neointima formation. Therefore, NFATs may be used as target molecules for the development of therapeutic agents against vascular diseases such as restenosis.
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