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by
Domati Anand Kumar |
Total Records (
2 ) for
Domati Anand Kumar |
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Ashok Kumar Tiwari
,
Iragavarapu Anusha
,
Maddineni Sumangali
,
Domati Anand Kumar
,
Kuncha Madhusudana
and
Sachin Bharat Agawane
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Aim: Increased consumption of sugar-sweetened beverages have been
identified as one of the major sources responsible for increasing global epidemic
of hyperglycemia, hyperglycemia induced oxidative stress and consequently, development
of number of diabetic complications. This research explored preventive and therapeutic
potentials of fruit juice of Benincasa hispida (BH) and Sechium edule
(SE) in fructose and sucrose-sweetened beverages induced Impaired Glucose Tolerance
(IGT), oxidative stress and IGT induced other metabolic disturbances in rats.
Materials and Methods: Rats were orally administered fructose and sucrose
sweetened-beverage prepared in water and respective fruit’s juice for one
month. A group of rats were maintained for three months on daily single dose
of sucrose. Three months sucrose-fed rats were later treated with fruit’s
juice for fifteen days. Degree of IGT development was determined following Oral
Glucose Tolerance Test (OGTT). Shape of plasma glucose concentration curve following
OGTT, glycemic load, plasma Total Antioxidant Potential (TAOP), oxidative stress
status, platelet aggregation activity and, Advanced Glycation End-products (AGEs)
level in blood of rats were examined. Results: Fructose was more potent
in inducing IGT than sucrose. Sucrose was more potent in decreasing plasma TAOP
and inducing oxidative stress than fructose. BH juice prevented development
of IGT induced by fructose and also significantly (p<0.05) reduced two hour
glycemic load following OGTT. Three months continuous single dose administration
of sucrose in rats induced development of IGT, increased two hour glycemic load
(p<0.05) following OGTT, decreased TAOP (p<0.05), increased oxidative
stress (more than twice) when compared with normal rats. Treatment of theses
rats with juice of BH and SE accelerated normalization of oral glucose tolerance
ability and decreased oxidative stress, platelet aggregation and accumulation
of AGEs in sucrose induced IGT and oxidatively stressed rat. Conclusion:
Our observations reveal that consumption of BH and SE juice may help reduce
development of hyperglycemia and hyperglycemia induced development of complications
by virtue of their antihyperglycemic and antioxidative stress potentials. |
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Ashok Kumar Tiwari
,
Domati Anand Kumar
,
Pisupati Sri Rajeshwari Sweeya
,
Kondepudi Manikanta Abhinay
,
Anusha Chauhan Hanumantha
,
Vadala Lavanya
,
Ambati Sudharshana Raju
,
Kayyuru Sireesha
and
Konda Pavithra
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Aim: Insulin resistance and leptin resistance are hallmarks in common for development of type 2 diabetes mellitus (T2DM) and obesity. Both these conditions are associated with increased activity and expression of Protein-Tyrosine Phosphatase 1β (PTP 1β). Therefore, inhibition of PTP 1β activity or down-regulation of its expression has become an attractive area of therapeutics development in order to ameliorate phenomenon of insulin and leptin resistance. This research analysed and assessed PTP 1β inhibitory activity in thirteen easily available and commonly consumed vegetables’ juice. Materials and Methods: Percentage yield of the juice, total polyphenol and total protein concentrations were estimated in fresh juice of vegetables. PTP 1β inhibitory activity by vegetables’ juice in cytosolic supernatant of goat liver and skeletal muscle was determined. Results: Wide variations in juice yield, total polyphenol and protein concentration in vegetables’ juice were found. Juice of Ridge Gourd (RG) and Yellow Pumpkin (YP) were rich source of both, polyphenol and protein. Green Cucumber (GC), Bottle Gourd (BG), Snake Gourd (SG), RG, Radish (RD), Carrot (CT), Noolkol (NK) and Yellow Cucumber (YC) displayed reversible inhibition of PTP 1β. Juice of vegetables YP, Ivy Gourd (IG), Chayote (CH), Ash Gourd (AG) and Banana Stem (BS) displayed irreversible PTP 1β inhibition. Based on the order of PTP 1β inhibition potential in both liver and skeletal muscle, juice of RD and BG were identified most potent. Conclusion: This research finds vegetables’ juice as potent source of PTP 1β inhibitor and may offer cost effective therapeutic potential in reducing risk of insulin resistance and development of T2DM. |
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