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Articles by Doha A. Mohamed
Total Records ( 4 ) for Doha A. Mohamed
  Sahar Y. Al-Okbi , Doha A. Mohamed , Thanaa E. Hamed , Ahmed M.M. Gabr , Hoda B. Mabrok , Shaimaa E. Mohammed and Oksana Sytar
  Background and Objective: Non-alcoholic fatty liver is recognized as the hepatic component of metabolic syndrome that accused for induction of cardiovascular and chronic liver diseases. This research evaluated the protective effect of two varieties of buckwheat seeds; Rubra and Karadag and their calli prepared by biotechnology towards non-alcoholic fatty liver in rat. Materials and Methods: Total phenolics, flavonoids, flavonols and DPPH free radical scavenging activity of buckwheat seeds and calli were assessed. Metabolic syndrome together with non-alcoholic fatty liver (MF) was induced by maintaining rats on high fructose diet. Daily oral dose of buckwheat seeds and calli (40 mg/rat) were given to 4 different groups during MF induction and compared to MF control rats and control group fed on balanced diet (NC). The experiment continued for 5 weeks. Results: In vitro free radical scavenging activities, total phenolic and flavonols of the calli were shown to be higher than the seeds. In vivo study showed significant dyslipidemia, significant increase in malondialdehyde, tumor necrosis factor-alpha, transaminase activity and liver fats in MF control group compared to NC. The relative gene expression of transforming growth factor beta-1 (TGF-β1) and TGF-β1 receptors I in liver as fibrogenic markers was significantly up-regulated in MF control group compared to NC. Treatment with different buckwheat forms produced improvement of the various determined plasma parameters and liver fats with variable degrees. Also, mRNA expression of TGF-β1 and TGF-β1 receptors I genes were significantly down-regulated in rats' liver on treatment with buckwheat seeds and calli. Conclusion: Buckwheat seeds and calli showed improvement of fatty liver and fibrogenic biomarkers.
  Doha A. Mohamed , Sherein S. Abdelgayed , Rasha S. Mohamed and Hend A. Essa
  Background and Objective: Hyperthyroidism and hypothyroidism are the most common disorders of thyroid function. The current study aimed to evaluate the prophylactic effect of safflower petals and moringa leaves crude ethanol extracts against thyroid dysfunctions (hyper and hypothyroidism). Materials and Methods: Forty-two rats were divided into 7 groups, control normal, hyperthyroidism control, hyper-safflower, hyper-moringa, hypothyroidism control, hypo-safflower and hypo-moringa. L-Thyroxine (0.3 mg kg1 b.wt.) and carbimazole (10 mg kg1 b.wt.) were orally administrated for 3 weeks as hyperthyroid and hypothyroid inducer, respectively. Blood hemoglobin, plasma thyroid-stimulating hormone (TSH), glucose, catalase activity, lipid profile as well as liver and kidney functions were assessed. Histological examination of thyroid gland was carried out. Results: The results revealed that hyper and hypothyroidism mediated decrease and increase in TSH values, respectively. Oral administrations of either safflower petals extract or moringa leaves extract improve plasma levels of TSH. Oxidative stress and disturbance in plasma glucose, lipid profile as well as liver and kidney functions were occurred in conjunction with thyroid dysfunctions especially hypothyroidism. Administration of safflower petals extract or moringa leaves extract alleviates the reduction in catalase activity, hyperglycemia and disturbance in lipid profile as well as liver and kidney functions accompanied with thyroid dysfunctions especially hypothyroidism. Conclusion: The studied extracts have prophylactic potential against thyroid dysfunctions and the subsequent oxidative stress, hyperglycemia and changes in lipid profile. Crude ethanol extract of safflower petals was promising as prophylactic agents in hyper and hypothyroidism as observed by improving plasma levels of TSH, lipid profile and histopathological changes.
  Doha A. Mohamed , Minar Mahmoud Hassanein , Tamer Mohammed El-Messery , Mohamed Tawfeek Fouad , Marwa Mohamed El-Said , Karem Aly Fouda and Adel Gabr Abdel-Razek
  Background and Objective: Olive pomace (OP) is the by-product of olive oil production. The OP contains large amount of phenolic compounds. The aim of the present study was to investigate the anti-diabetic effect of yoghurt supplemented with probiotics and olive pomace extract (OPE) in diabetic rats. Materials and Methods: Ultrasonic-assisted method was used for extraction of OP. Total phenolic content of OPE was estimated. The antibacterial activity of OPE was determined. Yoghurt supplemented with probiotics and OPE was prepared. Antioxidant activity, titratable acidity, water holding capacity (WHC) and organoleptic properties were evaluated in yoghurt. Anti-diabetic effect of yoghurt against type-2 diabetes was evaluated in rats. Blood samples were collected for determination of plasma glucose, insulin, lipid profile, plasma markers of oxidative stress, tumor necrosis factor-α, liver and kidney functions. Data were analyzed statistically using the one-way analysis of variance (ANOVA) followed by Duncan’s test. Results: The OPE contains 720 mg gallic acid equivalent g–1 dry weight. The HPLC analysis revealed the presence of many phenolic compounds such as vanillin and sinapic acid in OPE. OPE possess antibacterial effect against pathogenic bacteria. Antioxidant activity of yoghurt increased with the increment in OPE concentration. During storage the pH and acidity of yoghurt showed continuous decrease and increase, respectively. The WHC of fresh yoghurt decreased slightly with increasing OPE concentration, while WHC increased during storage. Hardness of yoghurt was affected non-significantly with increasing the concentration of OPE and storage periods until the 14th day. Organoleptic results revealed that yoghurt could be supplemented with OPE up to 1.5%. Diabetic rats showed significant changes in plasma glucose, insulin, lipid profile, TNF-α, oxidative stress markers and kidney function. Oral administrations of yoghurt supplemented with probiotic and OPE to diabetic rats showed significant improvement in all the studied biochemical parameters with different degrees. Conclusion: Yoghurt supplemented with probiotics and 1.5% OPE was the most promising in improving type-2 diabetes in diabetic rats.
  Sahar Y. Al-Okbi , Doha A. Mohamed , Eman E. Abd-Elhady , Ahmed M.S. Hussein and Enas S.K. Al-Siedy
  Background and Objectives: Non-alcoholic fatty liver disease (NAFLD) is accused as inducer of both cardiovascular and chronic liver diseases. The aim of the present study was to evaluate the therapeutic effect of combined freeze dried orange juice with its dried pulp supplemented with methionine, as functional food, in comparison to orange bioactive constituents, as parallel formula, in NAFLD rat model. Materials and Methods: Proximate composition, dietary fibers, minerals, total phenolics, fatty acids and phytosterols were determined in the orange functional food. The NAFLD was induced in rats through feeding high fructose diet. The prepared functional food and its parallel formula were evaluated in NAFLD rats through determination of liver fat and plasma lipid profile, malondialdehyde, tumor necrosis factor-α, leptin, insulin and glucose as well as liver and kidney function with histopathological examination of the liver. Insulin resistance (IR) and total cholesterol/high density lipoprotein cholesterol were calculated. Results: Orange functional food was shown to contain 9.17% dietary fibers, 1.4% potassium, 1.4 phenolic content as mg gallic acid/g, oleic acid as the major fatty acid (29.75% of total fatty acids) and 11.97% phytosterols from unsaponifiable matter. The studied formulas produced reduction of liver and plasma lipids, inflammatory and oxidative stress biomarkers, IR and leptin with improving liver function and histopathology pointing to potential management of NAFLD. Conclusion: Orange functional food and its parallel formula were promising in management of NAFLD; with superiority to orange functional food. Phenolic compounds, dietary fibers, phytosterols and mono and poly-unsaturated fatty acids could be responsible to the bioactivity of orange formula.
 
 
 
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