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Lipopolysaccharide is the most important component of Gram
negative bacteria that activates immune system through TLR-4 pathway. In the
present study, the adjuvant effects of Pasturella multocida 52 strain
(Pm52) on Hen Egg-white Lysozyme (HEL) and the possible attenuation of septic
signs by pravastatin were investigated. In vitro results showed that
Pm52 LPS stimulates nitric oxide (NO) production from splenocytes in time and
dose dependent manner where as pravastatin (10 μM) inhibited the LPS induced
NO production to basal levels (p<0.05). Furthermore, presence of pravastatin
(10 μM) does not inhibit the in vitro proliferative responses of
splenocytes by graded doses of Pm52 extracted LPS. The co-administration of
Pm52 LPS with HEL by subcutaneous route in mice significantly increased TNF-α
and NO levels at 2 and 6 h, respectively, as well as the HEL specific IgG development
at 14th day Post Inoculation (PI). Administration of pravastatin by intraperitoneal
route significantly reduced serum TNF-α and NO levels, the pro-inflammatory
mediators responsible for induction of septic shock as well as stabilized TNF-α:
IL-10 ratio without affecting HEL specific adaptive immune responses. However,
further studies are required in this direction to determine the long term immune
modulating effects of LPS and pravastatin on the host animals so as to be used
in a beneficial way.
