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Articles by Dina Zein El-Abdin Abdel-Kader
Total Records ( 2 ) for Dina Zein El-Abdin Abdel-Kader
  Dina Zein El-Abdin Abdel-Kader
  The present research was aimed to investigate whether Nitric Oxide (NO) is involved in zinc homeostasis in plants. It also aimed to study the involvement of GSH, SH groups and NO in mediating changes in zinc homeostasis. Wheat seeds (Triticum aestivum L., Cultivar giza-167) and bean seeds (Phaseolus vulgaris, cultivar Bronco) were germinated and grown in vermiculite watered with nutrient solution. Zinc was supplied in different concentrations (0.5, 2.7, 5.4 (control), 10.8 and 21.6 μM as ZnSO4). Sodium Nitroprusside (SNP) was used as NO donors in a 100 μM concentration and Methylene Blue (MB), in a 100 μM concentration, was used as NO scavengers. The solutions were supplied to plants by irrigation once a week included in the nutrient solution. 21-d-old plants were harvested. The results revealed that SNP application (NO donor) alleviated the adverse effect of deficient and toxic levels of zinc concentrations by increasing shoot and root biomass allocation, RGI% and protein content. Moreover, SNP application maintains a suitable zinc concentration in both deficient and toxic levels of zinc supplies in both wheat and bean seedlings. Nitric oxide induced significant changes in total and free/total SH, GSH content and SOD activity. The effect of nitric oxide was supported by using MB which scavenges the endogenous nitric oxide. The effect of NO is suggested to be a result of the adjustment of total or free SH levels, glutathione content and SOD activity.
  Dina Zein El-Abdin Abdel-Kader
  The present study was aimed to investigate the role of NO in iron homeostasis and to evaluate the ability of NO to cope with iron deficiency or toxicity symptoms in two wheat (Triticum vulgaris L. Sids-1 and Gimmieza-7) cultivars. Seeds were germinated and grown in vermiculite watered with nutrient solution. Iron was supplied in different concentrations (zero, 20, 40, 80 and 160 μM) as Fe-EDTA. Sodium Nitro-Prusside (SNP) was used as NO donors in a 100 μM concentration and 100 μM Methylene Blue (MB) was used as NO scavengers. The solutions were supplied to plants by irrigation once a week included in the nutrient solution. The results revealed that NO plays a significant role in enhancing the availability of iron in iron-deficient conditions. It also ameliorates the adverse effect of excess iron in the two cultivars under investigation by increasing the activities of catalase and ascorbic acid peroxidase as well as NO concentrations and decreasing lipid peroxidation and protein degradation. Moreover, NO declined iron stress-induced chlorosis symptoms by increasing Chl. a+b concentration. NO was found to affect Chl. a+b content by enhancement of the conversion of Mg-protoporphyrin to pchlide and subsequently to chlorophyll. The role of NO was approved by using NO scavenger (MB) which reverses all NO effects when applied.
 
 
 
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