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Articles by Diky Setya Diningrat
Total Records ( 5 ) for Diky Setya Diningrat
  Diky Setya Diningrat and Erly Marwani
  Background and Objective: Buasbuas is the one of the medicinal plants in Indonesia that contains bioactive compounds potential as antimicrobial, antioxidant, antidiabetes, antiinflammation and anticancer. Exploring the pathway and gene related of buasbuas bioactive compounds production has led to the renaissance of understanding buasbuas molecular mechanism database. The aim of this study was to developed data-mining framework of buasbuas to study plant specialized metabolism for phytochemical biosynthesis. Material and Methods: This project was started by collecting shoots and leaves of Buasbuas. Focus of the project was exploring the molecular mechanisms on biosynthesis phytochemical of Buasbuas. Illumina Mi-Seq Next Generation Sequencing was utilized to understand the molecular mechanisms of biosynthesis. Transcriptomes then trimmed and assembled with CLCBio genomic software. Assembled contigs then annotated towards Arabidopsis thaliana using CLC Bio genomic software. Digital Gene Expression was performed to analyze the transcriptional changes in control culture and treatment. Results: There were 5.342 unigenes that expressed only in treatment shoot cultures. Annotation with Gene Ontology showed that 57.9% (3.446) unigenes play role in Biological Process, 56.7% (3.375) unigenes play role in Cellular Components and 63.4% (3.772) unigenes play role in Molecular Functions. Annotation with Kyoto Encyclopedia of Genes and Genomes shows 853 unigenes essentially have role in 24 biological pathways. Highest process with highest unigenes involvement is biosynthesis of plant hormones and biosynthesis of alkaloids. Conclusion: This study showed that phytochemical biosynthesis in buasbuas induces level expression of several genes involved in the jasmonic acid, cytokinin, gibberellin, salicylic acid and ethylene biosynthesis pathway.
  Martina Restuati and Diky Setya Diningrat
  Background and Objective: North Sumatera Indonesia has a rich heritage of knowledge on medicinal plants used for preventive and curative medicine Premna pubescens. Blume (Buasbuas) has been used to increase the body immunity and endurance. Centella asiatica (Pegagan) is used for medicinal purposes. This study is important to find out the antimicrobial capabilities of Premna pubescens (P. pubescens) and Centella asiatica (C. asiatica) methanol extracts. This study is expected to provide the scientific foundation for the development of plants that are traditionally believed to be efficacious drug. The aim of the study was to investigate in vitro antimicrobial activity of North Sumatera medicinal plants P. pubescens and C. asiatica against the main pathogens. Materials and Methods: The organic solvent plant extracts are tested on the various microorganisms including bacteria and fungi by using agar diffusion technique. The data was analyzed with ANOVA statistics by using SPSS software. Results: The length of the inhibition zone was measured in mm from the edge of the well to the inhibition zone. P. pubescens showed significant moderate activity against (14 mm) Pseudomonas marginalis and (21 mm) Streptococcus mutans with 100 mg mL–1 DMSO plant drug concentration. The results of lowest (MICs) values are at 66 and highest ones are at 152 mg mL–1 for P. pubescens meanwhile those of (MICs) values are 0-155 mg mL–1 for C. asiatica. Conclusion: In general, based on the result of this research, it can be said that P. pubescens and C. asiatica plants can be used as antibacterial and antifungal compounds.
  Novita Sari Harahap , Aznan Lelo , Ambrosius Purba and Diky Setya Diningrat
  Background and Objective: Explosive power movement in a 200 m race will potentially yield free radicals. The energy source of a 200 m runner, based on his movement, comes from anaerobic predominant metabolism, which also potentially yield high lactic acid. The increase in lactic acid causes the decrease in pH which tends to suffer from acidosis so that the establishment of ATP is hampered and cause pain in muscles. This condition can influence the performance of the 200 m runners. The objective of the research was to find out the influence of combination of weight training with sport massage, on the change in glutathione peroxides (GPX) and lactic acid elimination in the 200 m runners. Materials and Methods: The research used experimental method with pre and post test design. The research subjects were 20 students of Faculty of Sports Science, Medan State University, who had not been trained as 200 m runners and had fulfilled the inclusive and exclusive criteria, taken by using random sampling technique. Students were divided into two groups: WT+SM (Combination of weight training with sport massage) group and WT-SM (weight training without sport massage). The treatment was performed in 8 weeks with the frequency of exercise 3 times a week. Results: The research showed that there was significant influence of the treatment (p<0.05) in the pre and post treatment in the WT+SM group and in the WT-SM group on the mean value of increase in glutathione peroxides content and the mean value of decrease in lactic acid content in 200 m runners. There was no significant difference (p>0.05) of the increase in glutathione peroxides in 200 m runners in the post treatment done by WT+SM group and by WT-SM group. There was significant difference (p<0.5) in the mean value of decrease in lactic acid content in the 200 m runners in the post treatment done by WT+SM group and by WT-SM group. Conclusion: Combination weight training with sport massage was better in increasing glutathione peroxides content and decreasing lactic acid content in the 200 m runners than that of only weight training.
  Eri Mustari , Diky Setya Diningrat , Rina Ratnasih and Srinanan M. Widiyanto
  Background: Teak (Tectona grandis Linn f.) is one of valuable timber species because of the beauty, strength and durability properties. Main problem in teak improvement program is reproductive biology, a low pollen viability, high embryo abortion, low pollination success and low fruit production. Investigation carried out at the teak improvement that flowering aspects is very important. Teak development program was facing difficulties due to the lack of information about the role of genes regulating flowering. APETALA2 (AP2) and APETALA3 (AP3) genes as member of the floral organ Identity. The role of teak flowering development predicted by up and down regulation of TgAP2 and TgAP3 genes. This study was conducted to determine the gene expression profile of TgAP2 and TgAP3 in the developmental stages of teak flowering. Materials and Methods: In this study, TgAP2 and TgAP3 genes in teak identified from NGS transcriptome data that is annotated with Solanum lycopersicum. The TgAP2 and TgAP3 genes activities on the several developmental stages of teak flowering tissues are done with QRT-PCR analysis using 18S RNA as gene reference. Results: The highest expression level of TgAP2 on the floral development stages of 4th lateral floral buds. The TgAP3 gene expression reached the highest level on the apical floral bud stage development. Vegetative shoot as control stages, TgAP2 gene expression level reached 9 times compared to the control and TgAP3 more than 12 times. The TgAP2 and TgAP3 genes expression profile has non-significant correlation on all floral development stages. Conclusion: The TgAP2 and TgAP3 genes expressed with different level in all stages of the teak flowering development. The TgAP2 and TgAP3 genes have non-significant correlation expression profile in role of teak floral development. These results indicate that the TgAP2 and TgAP3 expression profiles equivalent to the general pattern in the Arabidopsis model plant.
  Martina Restuati , Ulfa Hidayat , Ahmad Shafwan S. Pulungan , Nanda Pratiwi and Diky Setya Diningrat
  Background and Objective: Premna pubescens blume popularly known as buasbuas belonging to the family Lamiaceae, wide-spread in the forests of Sumatra and Malaya peninsula. The preliminary screening of the ethanol extract of P. pubescens revealed the presence of alkaloids, steroids, flavonoids and phenolic. Previous studies revealed that there is no systematic study regarding the antimicrobial activity of leaf extracts of P. pubescens. The aim of this study was to assess the in vitro effect of leaf extracts of P. pubescens against Bacillus cereus and Escherichia coli causative agent of diarrheal diseases. Materials and Methods: Fresh leaves were procured from personal P. pubescens plant collections aged 5-7 years. Crude ethanol extract of leaves from P. pubescens were tested in vitro against B. cereus and E. coli at concentrations 10, 20, 30, 40 and 50 μg μL–1. Results: The B. cereus and E. coli showed concentration-dependent susceptibility towards the ethanol leaf extracts from P. pubescens. The degree of susceptibility varied depends on the concentrations, 50% concentration of P. pubescens leaf extracts showed the highest inhibition zone (12.5 and 13.6 mm diameter). Conclusion: Based on the current findings, it can be concluded that P. pubescens has antimicrobial activity which is as potent as standard antimicrobial drugs against B. cereus and E. coli. The antibacterial properties of P. pubescens leaves were not as effective as the commercial antibiotics chloramphenicol, enrofloxacin and penicillin. Nevertheless, future studies with higher extract concentrations, different method of extraction, properties from flowers, fruits, barks and roots extracts may be useful to evaluate the actual antibacterial performed to other pathogenic bacteria.
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