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Articles by Dikayani
Total Records ( 4 ) for Dikayani
  Dikayani , Anas , Anne Nuraini and Warid Ali Qosim
  Background and Objective: Banana plants are agricultural crops, particularly horticulture, mostly consumed by many people. Banana plants are considerably influenced by biotic and abiotic environmental factors. Stresses caused by abiotic factors, is one of which caused by salinity, will result in decreased production of bananas, because these plants are very sensitive to salinity. This study aimed to investigate the response of banana plants to salinity stresses in vitro culture. Materials and Methods: Explants of banana Musa acuminate L. var. Barangan and basic medium murashige and skoog, benzyl amino purine, sugar, jelly, NaOH, HCl were used. Statistic analyzed with analysis of variance and Duncan test. The experimental design used was randomize complete design with two factors, factor I: Sodium chloride (NaCl) treatments of various concentrations, i.e., 0, 50, 100, 150 and 200 mM and factor II: Time after culture, in 1, 2, 3 and 4 weeks after culture. Observations over growth parameters were performed on height, wet weight and dry weight of banana plantlets at 1, 2, 3 and 4 weeks after cultures. Statistical analysis was done by one way analysis of variance. Results: The statistical analysis showed no significant differences in the plant height, salinity inhibits the growth of banana plantlets in term of the parameters of height, wet weight of shoots and roots, dry weight of shoots and roots of the banana plantlets. A treatment of 200 mM NaCl indicated that plantlet growth is inhibited in term of height (2.6500 cm), wet weight of shoots (0.0917 g) and roots (0.1957 g) and dry weight of shoots (0.0096 g) and roots of banana plantlets (0.02009 g). Conclusion: Plant growth in saline stressed concentration of NaCl 50, 100 and 150 mM.
  Dikayani , Sri Nanan B. Widiyanto , Erly Marwani and Rina Ratnasih
  Environment has influenced the growth and development of agricultural crops including banana (Musa acuminata L.). Environmental stresses are caused by biotic factors such as fungi, bacteria and herbivore and abiotic factors such as temperature, water, light and salinity. Salinity stresses cause a decrease in the production of banana, because they affect the growth of the plant in the changing balance of Na+ and Cl‾ ions and changes in the plant’s primary metabolites including fatty acid compounds. The purpose of the research was to identify the fatty acid compounds. The research method was conducted in 2 stages: phase (1) Developing in vitro culture of banana shoots with NaCl treatment of 0, 50, 100, 150 and 200 mM concentrations, (2) Gas Chromatography Mass Spectrometry (GCMS) analysis on the roots of in vitro banana planlets. The results showed that the fatty acid compounds involved in the protection against salt stress. The fatty acid the highest area compound in 0 mM treatment 9 Octadecanoic acid methyl ester were (14:36%), 50 mM treatment 9-octadecanoic acid methyl ester 13.13% and 100 mM treatment Tridecanoic acid 11.8%. In 150 mM treatment 9,12 octadecanoic acid 31.03%, whereas, the highest area in 200 mM treatment 9,12 octadecanoic acid 36.49%.
  Dikayani , S.N. Widiyanto , E. Marwani and R. Ratnasih
  Musa acuminata L. growth has influenced by environment. Environmental stresses are caused by biotic factors such as fungi, bacteria and herbivore and abiotic factors such as temperature, water, light and salinity. Salinity stresses cause a decrease in the production of banana. The purpose of the research was to validity and confirmation 4-Coumarate-CoA ligase Like5 (4CLL5) gene with 18S reference genes as a control genes on planlets of banana with sodium chloride (NaCl) application. 4-Caomarate-CoA-ligase Like5 is an enzyme key in phenyl-propanoid metabolism like lignin and flavonoid that essential for plant defense on abiotic factor like salinity is coded by 4CLL5 on Musa acuminate L. Salinity is abiotic factors which affect the growth of banana plant. Gene expression 4CLL5 on banana by using real-time quantitative polymerase chain reaction. Research using shoot banana in vitro culture with any concentration of sodium chloride (NaCl) were 0.00, 0.05, 0.10, 0.15 and 0.20 M. In addition to, in this research was using 4CLL5 as a primers. The expression of genes analysis was using real- time quantitative polymerase chain reaction. The results showed that 4CLL5 gene was expressed up regulated on NaCl 0.05 and 0.1 M but down regulated in 0.15 and 0.20 M.
  Dikayani , Anas , Anne Nuraini and Warid Ali Qosim
  Background and Objective: Quality banana fruit is significantly related with number and type of metabolite produce. Metabolite production of Musa acuminata L. is effected by abiotic factors such as salinity. So the purpose of this research was to study the effect of plant response under salinity stress and also identified the chemical compound especially phenolic compound. Materials and Methods: Explants used in this research were shoot of Musa acuminata L., Murashige and Skoog medium, Benzyl amino purine, sugar, agar, sodium chloride. The research was conducted in two stages; stage (1): Developing in vitro culture of shoot of Musa acuminata L. with NaCl treatment i.e., 0, 50, 100, 150 and 200 mM concentration, stage (2): Gas Chromatography Mass Spectrometry (GCMS) analysis on the shoot of in vitro banana plantlets. Results: The findings of this study showed that compound content of shoot of banana plantlets were Amine, Ester, Propane, Keton, Alkohol, Phenol, Methyl, Acid and it was observed that Ester (27.515%) was the highest detected compound. Conclusion: It is concluded that the salinity had affected the plant growth. The analysis showed that the highest compound content of Musa acuminate L. Barangan cultivar was Ester and Phenols.
 
 
 
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