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Articles by Di Wu
Total Records ( 6 ) for Di Wu
  Qi Zhang , Guangji Wang , Jiye A , Bo Ma , Yu Dua , Lingling Zhu and Di Wu
  This study describes the metabolic profiles of the development of hyperlipidaemia in a rat model, utilizing metabonomics by gas chromatography–mass spectrometry (GC-MS) determination coupled with multivariate statistical analysis. Rat plasma samples were collected before and during a high-lipid diet at days 0, 7, 14, 21 and 28, and were analysed for lipid levels using kit assays or metabonomics using GC-MS. Forty-one endogenous metabolites were separated, identified and quantified using GC-MS. The data matrix was processed by principal component analysis or partial least squares discriminant analysis. Dynamic modification of the rat metabonome can be clearly identified and tracked at different stages of hyperlipidaemia in the rat model. Potential biomarkers, including β-hydroxybutyrate, tyrosine and creatinine, were identified. The current work suggests that metabonomics is able to provide an overview of biochemical profiles of disease progress in animal models. Using a metabonomic approach to identify physiopathological states promises to establish a new methodology for the early diagnosis of human diseases.
  Zhetao Li , Renfa Li , Di Wu and Conte Mohamed
  In multimedia wireless sensor networks, multiple packets are generated for the same destination. All these packets go through the same route in a session when using current geographical routing. However, detour is inevitable in geographical routing without the help of global state. A progressive yet effective strategy is proposed to mitigate inefficient detour in geographical routing. In asymptotically optimal geographical routing, detour mode was substituted by greedy mode with the help of a subset of nodes acting as way points. The average performance of the proposed algorithm is compared to Greedy Perimeter Stateless Routing (GPSR) and the benchmark shortest path algorithm. Simulation results show that in average the proposed algorithm can reduce as much as 50% of hops on the routes obtained by GPSR.
  Di Wu , Guanglei Li , Ruiyi Lin , Changchun Li and Shuhong Zhao
  HOXa-10 (HomeoboxA10) gene plays an important role in embryo implantation and development regulated by Progesterone (P4) which is secreted mainly through corpus luteum cells to regulate the embryo development. The study mainly investigated the effect of progesterone on the expression of HOXa-10 in the endometrium during the embryo implantation. Kunming mice were injected progesterone (2 or 4 mg/each per day), respectively from early pregnant D1 (the 1st day of pregnancy) to D4 (the 4th day of pregnancy) or D6 (the 6th day of pregnancy) by intraperitoneal injection. The endometrium samples of early pregnant D4, D5 (the 5th day of pregnance) and D6, from 3 groups were collected with each group containing 3 mice. Real-time fluorescence Quantitative PCR (QPCR) was used to analyze the expression of the mouse HOXa-10 gene. The result demonstrated that the expression level of HOXa-10 was increased at pregnant D4 and there was a peak at pregnant D5 then it began to decrease at pregnant D6. Besides, the expression level of HOXa-10 was dependent on the dose of progesterone.
  Shicai Shen , Gaofeng Xu , Fushou Chen , David Roy Clements , Xinghui Gu , Sigui Ji , Limeng Zhang , Hailin Yang , Fudou Zhang , Kesuo Yin , Hongmei Zhang , Jiarui Li and Di Wu
  Background and Objectives: The biocontrol agent Aphidius gifuensis has widely been released and applied since 1997 to control Myzus persicae in tobacco fields of Yunnan Province, southwestern China, but its ecological effects on the local insect community and diversity have not been described. Biological control agents may effect a diversity of organisms as well as the target for control but such trophic cascades have seldom been studied. This study aimed to explore the effects of A. gifuensis on the insect community and diversity in tobacco fields after long-term release and expansion of the biocontrol agent in Yunnan Province. Materials and Methods: The effects of A. gifuensis on species composition, importance value, species richness, diversity indices and evenness index were studied for insect community samples obtained via field surveys and malaise trap collection in Yunnan Province. Results: A total of 39 insect species and 1 spider, principally belonging to 40 families and 13 orders in tobacco fields of Yunnan Province were identified. Among these, there were 20 pests, 14 natural enemies and 6 others, constituting 50, 35 and 15% of total species sampled, respectively. Within insect communities, M. persicae, Empoasca flavescens and Drosophila melanogaster were the dominant species and Spodoptera litura, one unidentified species (Ichneumonidae), Sphex haemorrhoidalis, Vespa veutinaauraris, Aedes albopictus, A. gifuensis and Liriomyza huidobrensis were the subdominant species. From June to August, the relative density of A. gifuensis gradually increased and concomitantly the total relative density of other natural enemies exhibited a slight decline in July and marked increase in August. The relative density of M. persicae significantly increased in July and then declined precipitously in August. Moreover, the values of Simpson index, Shannon-Wiener index and Pielou index were greater than 0.50, 1.50 and 0.50, respectively, indicating high insect diversity. Conclusion: Beneficial insects, in addition to the biocontrol agent are relatively abundant in tobacco fields of Yunnan Province, marking improvement in the local insect community diversity after a long-term release of the biocontrol agent A. gifuensis, which, by land area, constitutes one of the largest natural enemy control programs in the world.
  Hui Chen , Xiaojuan He , Zhun Wang , Di Wu , Huiyuan Zhang , Chunping Xu , Hongbin He , Lianxian Cui , Denian Ba and Wei He
  Human T lymphocytes, bearing T cell receptor (TCR) γδ, play an important role in anti-tumor/microbe immune responses. However, few tumor antigens recognized by TCRγδ have been defined so far. To investigate antigenic epitopes/proteins recognized by γδT cells, we have established a new immunobiochemical strategy that uses complementarity-determining region 3 of TCR δ chain (CDR3δ) peptide-mediated epitope/protein-binding assays. CDR3δ peptides synthesized using the CDR3 region in TCR Vδ2 chain were validated for their binding specificity to target cells or tissues. These CDR3δ peptides were then employed as probes to pan putative epitopes in a 12-mer random peptide phage-displayed library and to identify putative protein ligands within tumor protein extracts by affinity chromatography and liquid chromatography/electrospray ionization-tandem mass spectrometry analysis. As a result, we have identified nine peptides and two proteins for TCRγδ, including human mutS homolog 2 (hMSH2) and heat shock protein (HSP) 60. All nine tested epitope peptides not only bind to γδT cells but also functionally activate γδT cells in vitro. Identification of HSP60 confirms the validity of this method as HSP60 is an identified ligand for TCRγδ. We show that hMSH2 is expressed on the surface of SKOV3 tumor cells, and cytotoxicity of Vδ2 γδT cells to SKOV3 cells was blocked by anti-hMSH2 antibody, suggesting that hMSH2 may be a new ligand for TCRγδ. Taken together, our findings provide a novel immunobiochemical strategy to identify epitopes/proteins recognized by γδT cells.
  Michael Asiedu , Di Wu , Fumio Matsumura and Qize Wei
  We reported previously that a guanine nucleotide exchange factor, MyoGEF, localizes to the central spindle, activates RhoA, and is required for cytokinesis. In this study, we have found that Plk1 (polo-like kinase 1) can phosphorylate MyoGEF, thereby recruiting MyoGEF to the central spindle as well as enhancing MyoGEF activity toward RhoA. The in vitro kinase assay shows that Plk1 can phosphorylate MyoGEF on threonine 574. Immunoprecipitation/immunoblot analysis demonstrates that mutation of threonine 574 to alanine dramatically decreases threonine phosphorylation of MyoGEF in transfected HeLa cells, suggesting that threonine 574 is phosphorylated in vivo. Consistent with these observations, immunofluorescence shows that Plk1 and MyoGEF colocalize at the spindle pole and central spindle during mitosis and cytokinesis. Importantly, RNA interference-mediated depletion of Plk1 interferes with the localization of MyoGEF at the spindle pole and central spindle. Moreover, mutation of threonine 574 to alanine in MyoGEF or depletion of Plk1 by RNA interference leads to a decrease in MyoGEF activity toward RhoA in HeLa cells. Therefore, our results suggest that Plk1 can regulate MyoGEF activity and localization, contributing to the regulation of cytokinesis.
 
 
 
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