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Articles by Dewi Apri Astuti
Total Records ( 8 ) for Dewi Apri Astuti
  Suharlina , Dewi Apri Astuti , Nahrowi , Anuraga Jayanegara and Luki Abdullah
  This study aimed to evaluate nutritional quality of total mixed rations containing Indigofera zollingeriana for dairy goats by using in vitro rumen fermentation technique (RUSITEC). Four rations with different levels of I. zollingeriana were tested, i.e., 20% of dry matter (R1), 40% (R2), 60% (R3) and 80% (R4). Each ration was repeated three times by following a randomized complete block design in which different batch of rumen fluid served as the block. Variables observed included chemical composition, In Vitro Dry Matter Digestibility (IVDMD) and In Vitro Organic Matter Digestibility (IVOMD), methane emission, Volatile Fatty Acid (VFA) profiles and protozoa population. Results revealed that R4 contained significantly higher crude protein content than that of R1-R3 (p<0.05). Rations containing higher proportions of I. zollingeriana (60 and 80% dry matter) had significantly higher IVDMD and IVOMD as compared to their lower proportions (20 and 40% dry matter, p<0.05). Methane concentration was lowest in ration containing the highest proportion of I. zollingeriana and it was accompanied with the lowest protozoa population. It can be concluded that higher inclusion levels of I. zollingeriana in rations improved their nutritional values while decreasing methane emission as a main greenhouse gas.
  Agus Susanto , Erika B. Laconi , Dewi Apri Astuti and Syamsul Bahri
  The objective of the study was to evaluate and compare the efficiency of glucomannan extracted from Amorphophallus oncophyllus (GRE) with commercial aflatoxin binder glucomannan yeast product (GYP) in broiler chicken fed aflatoxin contaminated feed. A total of 63 one day old chicks were assigned to 9 equal groups according to dietary treatments: A (basal feed), B (basal feed+aflatoxin 50 μg/kg), C (basal feed+aflatoxin 2 mg/kg), D (basal feed+aflatoxin 50 μg/kg+GYP 1 g/kg), E (basal feed+aflatoxin 50 μg/kg+GRE 1 g/kg), F (basal feed+aflatoxin 50 μg/kg+GRE 2 g/kg), G (basal feed+aflatoxin 2 mg/kg+GYP 1 g/kg), H (basal feed+aflatoxin 2 mg/kg+GRE 1 g/kg) and I (basal feed+aflatoxin 2 mg/kg+GRE 2 g/kg). The body weight decreased significantly (p<0.05) with the inclusion of aflotoxins in the diet at 2 mg/kg diet. Both GYP and GRE were found almost equally effective in combating the negative impact by improving the body weights. Feed intake and FCR of birds fed basal diet with aflatoxin at 2 mg/kg decreased significantly (p<0.05) when compared to the control. Supplementation of both GYP and GRE improved the feed intake and FCR of broiler chicken. Aflatoxins at 2 mg/kg (group C) significantly (p<0.05) decreased PCV but not at 50 μg/kg level. GYP and GRE supplementation helped to overcome the negative effect of aflatoxin by increasing PCV compared to group C. There was no significant (p>0.05) effect on Hb, MCHC and glucose levels among various treatment groups. The weight of liver varied significantly (p<0.05) among the treatment groups but no change in heart, spleen and kidney weights were observed. The highest increase in the relative weight of liver was seen in the group fed aflatoxins at 2 mg/kg level. Supplementation GYP and GRE helped in reducing the liver enlargement effect of aflatoxins. In conclusion, GRE proved to be an effective alternative to GYP particularly at the rate of 2 mg/kg in providing protection against aflatoxin contaminated feed in broiler chicken.
  Anuraga Jayanegara , Mohammad M. Sholikin , Della A.N. Sabila , Sri Suharti and Dewi Apri Astuti
  Background and Objective: Cricket contains high crude protein level but it also contains considerable amount of chitin that may impede nutrient digestion and decrease production performance of animal. This experiment aimed to decrease chitin content of cricket (C) through exoskeleton removal (CER) or by chemical extraction (CCE). Materials and Methods: Nutritional evaluation of cricket was performed in two experiments. In experiment 1, three forms of cricket were prepared, i.e., C, CER and CCE. These were subjected to chemical composition determination and in vitro rumen fermentation incubation as individual substrates. In experiment 2, C and CER were included in concentrate rations at different proportions to substitute soybean meal (SBM), i.e., R1 (concentrate containing 30% SBM), R2 (50% SBM was substituted by C), R3 (100% SBM was replaced by C) and R4 (100% SBM was replaced by CER). The concentrates were then evaluated in vitro for their rumen fermentation and digestibility characteristics. Data were analyzed with analysis of variance and Duncan’s test. Results: Cricket was high in crude protein(CP), ether extract (EE) and chitin contents. Removal of exoskeleton decreased CP and chitin contents of cricket. Chemical extraction of cricket increased its CP and completely removed its chitin. Main fatty acids observed in cricket were linoleic acid, palmitic acid, oleic acid and stearic acid and the composition was unaltered due to exoskeleton removal or chemical extraction. Cricket was relatively highly digestible and exoskeleton removal and chemical extraction did not further improve in vitro dry matter digestibility (IVDMD) and in vitro organic matter digestibility (IVOMD) of cricket. The R1 and R2 revealed similar IVDMD and IVOMD, but R3 and R4 resulted in lower values for both parameters than those of R1 and R2 (p<0.05). Conclusion: Exoskeleton removal or chemical extraction effectively reduced chitin content of cricket and the insect may be used to substitute SBM up to 50% in concentrate for ruminant.
  Lilis Khotijah , Komang Gede Wiryawan , M. Agus Setiadi and Dewi Apri Astuti
  This research was conducted to evaluate reproductive performance, cholesterol, glucose and progesterone of Garut ewes fed rations containing different levels of sun flower oil. Using completely randomized design, four ration treatments were given to thirty two ewes (BW 22.12±1.69 kg) with eight animals for each treatment. Four iso-protein rations were used i.e., control (SFO0) without sun flower oil; SFO2, SFO4 and SFO6 were the same as SFO0 but containing 2, 4 and 6% of sun flower oil, respectively. Estrous synchronization was conducted by double injection of Prostaglandin (PGF2α) 11 days apart before mating. Reproductive performance (litter size, ratio of male to female, mortality), cholesterol and glucose plasma were measured two weeks before mating and at the first month of pregnancy. Progesterone serum concentration were measured on day 25th, 28th and 31st of pregnancy, using radioimmunoassay. Number of embryo was detected using Ultrasonograph detector at the day 18-20th after mating. Result showed that plasma cholesterol concentration of ewes fed control ration was lower than ewes fed rations containing sun flower oil, while glucose concentration two weeks before mating was lower than those at the first month of pregnancy. Progesterone concentration was the same in all treatments on day 25th, 28th and 31st of pregnancy. Pregnancy rate and number of embryo in SFO2 and SFO6 was 75% and lower than in SFO4 (100%). The highest litter size was in SFO4 (225%) similar to the number of embryo detected by USG, with the male to female ratio of 3:1. However, the percentage of embryo mortality was still high (>20%). It is concluded that inclusion of 4% sun flower oil in the ration could improve reproductive performance (litter size and male sex ratio) and cholesterol concentration.
  Sri Suharti , Dewi Apri Astuti , Elizabeth Wina and Toto Toharmat
  This research was aimed to investigate the utilization of whole lerak extract to improve rumen fermentation, nitrogen retention and performance of beef cattle received high forage based ration. Experimental diet composed of forage (70%) and concentrate (30%). The in vivo study was conducted using 12 local beef cattle which were divided into three treatments ie three different levels of lerak extract (0, 100 and 200 mg/kg body weight) were added to the diet. Parameters measured were nutrient digestibility, volatile fatty acid (VFA) profile, NH3 concentration, microbial protein synthesis, feed intake and daily gain of beef cattle during 90 days of feeding trial. The addition of lerak extract up to the level of 200 mg/kg BW did not affect nutrient digestibility. Total VFA and propionate proportion increased (p<0.05) and the ratio of acetate: propionate decreased (p<0.05) with the addition of lerak extract. Concentration of NH3 in the rumen tended to decrease. Nitrogen retention, microbial protein synthesis, feed intake and daily gain of local beef cattle fed high forage ration tended to increase with the addition of lerak extract at the level up to 200 mg/kg BW. The addition of lerak extract at the level of 200 mg/kg BW increased average daily gain up to 12.5% compared to the control treatment.
  Sri Rahayu , Mohamad Yamin , Cece Sumantri and Dewi Apri Astuti
  This study evaluated the effect of a diet containing mung bean sprout waste (MBSW) as a fiber source and feeding time on the growth performance and physiological responses of Garut lambs. Feeding regimens generated by a factorial randomized block design with two factors- diet and feeding time-were assigned to twenty Garut male lambs aged 6-7 months (BW 15.42±2.42 kg). The two diets (D) formulated with a dry matter (DM) base were D1 (60% concentrate 1+40% natural grass) and D2 (60% concentrate 2+40% MBSW). Feeding times were in the morning (MF, 6:00-7:00 am) or the evening (EF, 5:00-6:00 pm). The animals were reared in individual cages and fed with 5% DM/kg body weight/day. Nutrient intake (DM, crude protein (CP), total digestible nutrient (TDN) and average daily gain (ADG) of lambs fed D2 was significantly (p<0.01) higher than that of lambs fed D1. The feed conversion ratio (FCR) of lambs fed with D2 at the EF feeding time was significantly improved (6.07, p<0.05) compared to lambs fed D1 at the MF and EF time, respectively (7.88 and 8.27), or lambs fed D2 at the MF time (7.84). Heart rates (HR) were higher in lambs fed D2 than lambs fed D1. In contrast, the respiration rate (RR) and rectal temperature (RT) were similar for all treatments. These results suggest that diets containing 40% MBSW as a fiber source together with an evening feeding time could increase growth performance while preserving normal physiological responses of Garut lambs.
  Deyv Pijoh , Sri Supraptini Mansjoer , Dondin Sajuthi , Dewi Apri Astuti and Irma Herawati Suparto
  Objective: This study was conducted to develop an animal model of obesity by monitoring the phenotypes of long-tailed monkeys (Macaca fascicularis) fed a high energy diet. Methodology: Fifteen adult male Long-Tailed Monkeys (LTM) weighing between 4 and 5 kg were divided into three groups of five that received one of three diets: Diet A: A commercial monkey chow diet, Diet B: Monkey chow supplemented with egg yolk and Diet C: In which wheat was the main ingredient and beef tallow was added. Body measurements, including body weight were collected monthly. Results: The LTM fed Diet B (Egg yolk-supplemented) consumed more food compared to those fed Diet A and C. Animals in the Diet B group had significantly (p<0.01) larger waist circumference, hip circumference, chest circumference and skin fold thickness compared to the other two groups. There was a close relationship between body weight and waist circumference (r = 0.863), chest circumference (r = 0.889) and hip circumference (r = 0.890). Based on the main component analysis, LTM fed Diet B had higher rates of obesity compared to LTM fed Diet A or C. Conclusion: Body characteristics of LTM fed different diets can be used to determine the presence of obesity. The LTM can serve as a useful animal model of obesity and findings from this model can be applied to studies of obesity in humans.
  Neng Nenden Mulyaningsih , Ariadne Lakshmidevi Juwono , Djarwani Soeharso Soejoko and Dewi Apri Astuti
  Background and Objective: Serum calcium (Ca) is maintained at a balanced level under normal circumstances by a homeostatic system. When the serum Ca level is high, the excess will be deposited in the bone; however, if the serum Ca level is low, Ca will be resorbed from the bone and Ca absorption in the intestine will be increased. As a result, serum Ca metabolism can affect bone morphometry characteristics and Ca status in the body. The aim of this study was to investigate the effect of a nano-calcium phosphate diet on osteoporotic rats after ovariectomy. Experiments were conducted to analyse the serum response to Ca in rats that were ovariectomized and treated with a nano-calcium phosphate diet; additionally, the relationship between the diet and both the bone morphometry characteristics and Ca status in the body was examined. Materials and Methods: Female rats (Rattus norvegicus) aged 12 weeks were ovariectomized or acted as a control (non-ovariectomized rats). Euthanasia was carried out on 13-week-old control rats and ovariectomized (OVX) rats at the ages of 15, 17, 19 and 21 weeks. The 21-week-old OVX rats were divided into 3 groups and were given a nano-calcium phosphate diet containing a nano-Ca content of 0.10% (diet A), 0.40% (diet B) or 0.70% (diet C). At the ages of 27, 29, 31, 33 and 40 weeks, euthanasia was carried out for the collection of the serum, femur and tibia. The serum mineral levels [calcium (Ca), phosphorus (P) and magnesium (Mg)], morphometric characteristics (mass, mass density, length and diameter) of the long bones and Ca status in the body were analysed. Results: The results showed that in the ovariectomized rats, serum Ca and P levels decreased at week 7, while the Mg levels fluctuated. Treatment with the 0.40% nano-Ca diet could increase serum Ca levels from the 6th week of diet administration (age 27 weeks). The overall femoral morphometry and tibia characteristics, in addition to the mass density data, showed values that increased with age. The highest Ca absorption was shown by the OVX rats that consumed the 0.40% nano-Ca diet. The difference between the consumed and absorbed amounts of Ca was shown by the Ca content in the faeces, which averaged 68.07% for calcium intake. Conclusion: The female Rattus norvegicus white rats exhibited osteoporosis based on serum mineral status seven weeks post-ovariectomy. The effects of the nano-calcium phosphate diet were first observed in the sixth week of diet administration.
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