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Articles by Desy Cahya Widianingrum
Total Records ( 2 ) for Desy Cahya Widianingrum
  Desy Cahya Widianingrum , Sarasati Windria and Siti Isrina Oktavia Salasia
  Antimicrobial resistance patterns and gene encoding for methicillin/oxacillin resistance (mecA) were determined in 73 Staphylococcus aureus. The isolates of S. aureus originated from bovine (39 isolates) from Peranakan Etawa (PE) or crossbred Etawa goats (24 isolates) and from patients of Sarjito hospital (10 isolates) in Indonesia. The identification of S. aureus was based on cultural and biochemical tests and an amplification of a specific section of the 23S rRNA gene and thermonuclease (nuc) genes. Staphylococcus aureus originating from human and bovine were more resistant than those of goat origin. Seventeen S. aureus (23.29%) were resistant to single antibiotic and 15 isolates (20.55%) showed resistance to two antimicrobial agents. Multi resistances were found in 26 (35.62%) of S. aureus isolates. Resistance to ampicillin was the most common finding (80, 76.92 and 41.67%), followed by gentamicin (30, 51.28 and 25%), oxacillin (50, 38.46 and 58.33%), tetracyclin (40, 28.21 and 16.67%) and erythromycin (40, 23.08 and 20.83%) for human, bovine and goat, respectively. By PCR amplification could be observed in 5 (12.82%) methicillin/oxacillin resistant (mecA) genes for bovine isolates, 1 (4.17%) goat isolates and 9 (90%) human isolates. These isolates were identified as methicillin resistant S. aureus (MRSA). Most of MRSA were resistant to oxacillin (60%), ampicillin (66.7%), tetracyclin (40%), erythromycin (33.3%) and gentamicin (20%) The resistancy of S. aureus to methicillin/oxacillin and the other antibiotics in the present study might help to understand the distribution of methicillin resistant S. aureus (MRSA) among human bovine and goat isolates and might help to control S. aureus infections.
  Sarasati Windria , Desy Cahya Widianingrum and Siti Isrina Oktavia Salasia
  This study aimed to identify staphylococcal mastitis milk in Peranakan Etawa (PE) or Etawa crossbred goats in Indonesia. Total of 93 milk samples used in this study, there were 49 (52.7%) subclinical and 3 (3.2%) clinical mastitis samples. From 52 subclinical and clinical mastitis samples could be isolated 20 (38.5%) staphylococci. Phenotypic identification were performed based on the Gram staining, Mannitol Salt Agar (MSA), catalase, Voges-Proskauer (VP) and coagulase tests. Molecular identification were determined using the Polymerase Chain Reaction (PCR) to detect several genes such as 23S rRNA, nuc and coa genes and confirmed with DNA sequencing. Based on phenotypic and genotypic identification, could be identified 10 Coagulase Positive Staphylococci (CPS) indicated Staphylococcus aureus and 10 Coagulase Negative Staphylococci (CNS) isolates, including Staphylococcus pasteuri (3 isolates), Staphylococcus xylosus (5 isolates) and Staphylococcus haemolyticus (2 isolates). From 3 clinical mastitis of PE goats in our study could be isolated three different staphylococci i.e., S. aureus, S. pasteuri and S. xylosus. This finding indicated that both CPS and CNS have important role as causative pathogen of mastitis in PE goats.
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