Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by Deshun Shi
Total Records ( 4 ) for Deshun Shi
  Zhengsan Wu , Qingyou Liu , Peng Zhu , Shuai Liu , Guihua Bian , Sufang Yang , Fenghua Lu and Deshun Shi
  Effects of Scriptaid (SCR), low toxicity Histone Deacetylase inhibitors (HDACi) on the histone acetylation and development of porcine Somatic Cell Nuclear Transfer (SCNT) embryos were examined in this study. Treatment of activated reconstructed SCNT embryos with 500 nM SCR for 12 h resulted in a significantly increase in the blastocyst development compared to untreated group (21.0% vs. 9.7%, p<0.05). Further, SCR treatment resulted in SCNT embryos at the 2-cell, 4-cell and blastocyst stage with higher acetylation level of H3K18 in comparison with untreated group and similar to fertilized counterparts. Measurement of four selected developmentally important genes (HDAC2, HAT1, OCT4 and Phosphoglycerate kinase 1 (PGK1)) in blastocysts showed that treatment of SCNT embryos with SCR resulted in an increase expression in HAT1 and decrease expression in HDAC2 and PGK1. A similar expression profile of these genes was found in blastocysts developed from SCR treated SCNT embryos and In Vitro Fertilization (IVF) embryos. These results suggest that SCR treatment can improve the development competence of porcine SCNT embryos and result in a similar expression pattern of genes related to histone acetylation and acetylation level of histone compared to IVF embryos.
  Kangle Yi , Chunjin Li , Qili Qin , Lu Chen , Yanling Sun , Huahai Chen , Haifeng Yan , Deshun Shi and Xu Zhou
  The factor in the germline alpha (FIGLA) is a basic helix-loop-helix transcription factor that is essential for folliculogenesis and regulates expression of zona pellucida genes in mouse and human. But bovine homologue has not as yet been confirmed experimentally. Here, researchers present the first cDNA cloning and transcript expression analysis of the bovine FIGLA gene. Using RT-PCR and quantitative real-time PCR, researchers revealed that expression within adult cattle tissues is limited to the ovary. The researchers found that Germinal Vesicle (GV) oocytes, Metaphase II (MII) oocytes, 4 and 8 cell embryos, morula and blastocysts were all shown to express mRNA for FIGLA and expression were different in bovine oocytes and IVF embryos at different stages (p<0.05) with the highest expression in Germinal Vesicle (GV) oocytes and lower expression in 8 cells and blastocysts embryos. There was no difference in mRNA levels for FIGLA gene among bovine IVF, PA (Parthenogenetic Activated) and NT (Nuclear Transfer) blastocysts (p>0.05). In situ hybridization the FIGLA RNA was only localized in ovarian follicle. The persistence of FIGLA in adult cowes suggests that it may regulate additional pathways that are essential for bovine ovarian and embryonic development.
  Nan Li , Fenghua Lu , Peng Zhu , Qingyou Liu , Jiangrong Jiang and Deshun Shi
  O-linked β-N-Acetylglucosamine Glycosylation (O-GlcNAc) is one of the main types of glycosylation in mammalian cells while Glucosamine (GlcN) is an O-GlcNAc substrate. Thus, effects of GlcN on the embryonic development, level of O-GlcNAc and related gene expression of buffalo embryos were examined in this study. Buffalo zygotes derived from In Vitro Fertilization (IVF) were randomly allocated into culture in the medium supplemented with different concentration of GlcN (0, 1, 2 and 4 mM) during the different culture period (0-72, 72-172 and 0-172 h). When GlcN was added to the medium in the culture period of 0-72 h after IVF, addition of 2 mM GlcN resulted in more zygotes developing to blastocysts (26.1%) in comparison with control (14.3%), 1 mM (13.6%) and 4 mM (11.3%) groups (p<0.05). However, the blastocyst yield decreased gradually when GlcN was added to the medium during 72-172 h of culture and decreased significantly when the concentration of GlcN was arrived at 4 mM (3.1 vs. 14.2%, p<0.05). When GlcN was added to the medium in the whole culture period (0-172 h) there were no significant difference in either cleavage rate or blastocyst yield among the four groups (p>0.05). Immunofluorescence analysis revealed that addition of 2 mM GlcN to medium from 0-72 h after IVF resulted in a significant increase (p<0.05) in the O-GlcNAc level of embryos at 2, 4, 8 cells and morula stage with the exception of blastocysts. QRT-PCR revealed that culture of zygotes with 2 mM GlcN in the culture period of 0-72 h after IVF resulted in a significant increase (p<0.05) in the expression of O-GlcNAc transferase gene in the embryos at the 2, 4, 8 cells and morula stage and did not affect the expression of O-GlcNAc-selective N-acetyl β-D-glucosaminidase gene. These results indicate that appropriate concentration of GlcN can improve the development of buffalo embryos and this action is stage dependent and mediated by O-GlcNAc transferase gene.
  Zhengsan Wu , Qingyou Liu , Peng Zhu , Shuai Liu , Guihua Bian , Sufang Yang , Fenghua Lu and Deshun Shi
  Effects of Scriptaid (SCR), low toxicity Histone Deacetylase inhibitors (HDACi) on the histone acetylation and development of porcine Somatic Cell Nuclear Transfer (SCNT) embryos were examined in this study. Treatment of activated reconstructed SCNT embryos with 500 nM SCR for 12 h resulted in a significantly increase in the blastocyst development compared to untreated group (21.0 vs 9.7%, p<0.05). Further, SCR treatment resulted in SCNT embryos at the 2, 4-cells and blastocyst stage with higher acetylation level of H3K18 in comparison with untreated group and similar to fertilized counterparts. Measurement of four selected developmentally important genes (HDAC2, HAT1, OCT4 and Phosphoglycerate Kinase 1 (PGK1)) in blastocysts showed that treatment of SCNT embryos with SCR resulted in an increase expression in HAT1 and decrease expression in HDAC2 and PGK1. A similar expression profile of these genes was found in blastocysts developed from SCR treated SCNT embryos and In Vitro Fertilization (IVF) embryos. These results suggest that SCR treatment can improve the development competence of porcine SCNT embryos and result in a similar expression pattern of genes related to histone acetylation and acetylation level of histone compared to IVF embryos.
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility