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Articles by Dengpan Bu
Total Records ( 2 ) for Dengpan Bu
  Dan Li , Jiaqi Wang , Fadi Li and Dengpan Bu
  Methanogens and protozoa in the rumen negatively affect rumen function by wasting ingested energy. It is desirable to modulate rumen fermentation by cost-effective dietary intervention. The objective of this study was to evaluate effects of unsaturated C18 fatty acids (oleic, linoleic and linolenic acids), either alone or in combination with malic acid on in vitro ruminal fermentation, protozoa and Methanobacterium formicicum. Rumen fluid collected from ruminally fisulated lactating Chinese Holstein cows served as the inoculum and the diet consisted of alfalfa hay and corn (50:50). The results showed addition of unsaturated fatty acids tended to increase fermentation pH (p>0.05) and degree of unsaturation of fatty acids tended to affect such effect on the pH (p>0.05). Acetate, butyrate, total VFA, total gas production and methane production decreased (p<0.01) with increasing degree of unsaturation of C18 fatty acids but addition of malic acid did not have any additive or synergistic effect except for propionate which was decreased by the addition of malic acid (p<0.01). Both malate and the unsaturated fatty acids, either alone or in combination, decreased methane production (p<0.01) with combination of these two types of acid further decreasing methane production. Except for oleici acid that decreased population of M. formicicum decreased (p<0.01), either protozoa or M. formicicum was affected by these fatty acid and malic acid. It was concluded that when used together, malic acid and fatty acids could reduce methane emission without negative impairing fermentation in vitro.
  Tong Qin , Haoyu Wang , Dengpan Bu , Haisheng Hao , Dong Wang , Weihua Du and Huabin Zhu
  The objective of this study was to evaluate the effect of a corn straw or mixed forage diet on endocrine, metabolism and lactation performance in periparturient Holstein cows. Twelve multiparous, periparturient Holstein cows were randomly assigned to two groups and fed a corn straw or mixed forage diet, respectively. The CS diet included 33.8% corn straw and the F:C ratio [Dry Matter (DM)] was 60:40. The MF diet included 3.7% Chinese wildrye, 28.4% alfalfa hay and 26.5% corn silage, the F:C ratio (DM) was 40:60. All cows were fed from weeks 3-8 and Body Weight (BW), Body Condition Score (BCS) and Dry Matter Intake (DMI) were recorded. Milk protein, fat, lactose and Somatic Cell Count (SCC) were determined twice weekly. Metabolite and hormone analyses of blood were made weekly. Results showed that dietary treatments had no detectable effects on BW, BCS, DMI and blood hormones measured. From the 6 weeks of lactation, cows fed MF diet produced more milk (p<0.05) and tended to produce more milk fat (p = 0.07) and protein (p = 0.10) compared with cows fed CS diet. The proportions of milk fat, protein and lactose (%) did not differ between the two dietary treatments (p>0.05). In addition, the milk SCC in the CS group was significantly higher (p = 0.02) than the MF group. Cows fed MF diet experienced more severe Negative Energy Balance (NEB) and had higher concentrations of β-Hydroxy Butyric Acid (BHBA) and Non-Esterified Fatty Acid (NEFA) than cows fed CS diet. Collectively, these results suggest that cows fed MF diet improve lactation performance of periparturient cows but this dietary treatment may have an adverse effect on NEB of periparturient cows.
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